Orthophthaldehyde as a fluorescent probe for the feasible and sensitive assay of heptaminol: application to dosage forms and real human plasma

Abstract

The antihypotensive drug heptaminol was determined using a spectrofluorimetric method and ortho-phthaladehyde as a fluorescence probe. The drug was mixed with the reagent in the presence of 2-mercaptoethanol and the reaction was carried out in slightly alkaline aqueous solution containing 0.1 M sodium hydroxide. The resulting product exhibited high fluorescence activity that was measured at 451 nm after excitation at 334 nm. The linearity range of the method was 5-100 ng ml À1 with a lower detection limit of 1.8 ng ml À1 . The procedure was evaluated according to the International Council of Harmonization guidelines. The proposed method was applied to analyze the drug in pharmaceutical tablets and oral drops. In addition, the present study represents the first spectrofluorimetric method for the determination of the cited drug in real human plasma. The method provided high recovery percentages without any interference from coexisting pharmaceutical excipients or the components of human plasma.

Green innovative fluorescence approach for the feasible and reliable assay of thiol-containing drugs; captopril as a model

“… 54 Therefore, the chain should be of reasonable length. In our laboratory, various compounds that contain aliphatic primary amino group such as gabapentin, 48 3-isobutyl gamma aminobutyric acid, 49 milnacipran, 50 and AMH 51 were analyzed through the isoindole formation reaction. However, the use of AMH was found to fulfil the aforementioned criteria of being has a side chain of appropriate length (C6) with two points of branching, one of them is close to the amino group.…”

“…The interaction of OPA with thiols, specially 2-ME and compounds containing primary amino group, was extensively used to determine primary amine compounds, [47][48][49][50][51] including pharmaceutical compounds. 52,53 Because of the essential presence of the thiol compound in the reaction vessel, the reaction could be possibly employed for the thiol-containing compound.…”