Quantitative Determination of Jatrophone in "Cachaca" Prepared with Jatropha elliptica

Abstract

The genus Jatropha (Euphorbiaceae) are used in medicinal plant therapy against skin diseases.1) Jatropha elliptica according to popular wisdom can be used in the form of a tincture prepared by maceration of subterranean stems in a sugar-cane derived spirit "cachaça", administered orally, for the treatment of venomous snake bites. Other uses include as an anti-inflammatory, and an anticholestheremic. Studies phytochemical in ethanolic extract of the subterranean stems showed identification of the diterpene jatrophone ( Fig. 1) and also others substances.2) Other studied in dichloromethanic extract was determined d-selinene. 3)Jatrophone is considered cytotoxic and tumor-inhibitory agent. 2,4,5) This substance has been the object of several scientific investigations, and, in Brazil, results have included reports on molluscidal activity, 6) relaxant action in rat portal veins, 7) inhibition of insulin, 8) and inhibition of plateled rich plasma aggregation. 9)Most of the Jatropha elliptica in "cachaça" used in Brazil have no information on their exact composition in relation as substances presents or on the recommended dosages, imposing serious risks to public health.HPLC equipment is widely disseminated in Brazil. We have developed an analytical procedure suitable for sample preparation and HPLC-UV analysis of jatrophone contents in "cachaça".The HPLC technique has been shown to be a very efficient system separating complex mixtures plants. HPLC methods have been reported for the determination of substances in callus cultures, vitro culture, serum, dermis, plants, citrus, essential oils and phytomedicines, but only the most recent published methods report assay validation. [10][11][12][13][14][15][16][17][18][19][20][21] There are no descriptions in the literature about sample preparation and determination quantitative of jatrophone present in "cachaça" prepared with Jatropha elliptica employed in Brazil.The sensitivity, linearity, accuracy, precision were evaluated. The efficiency of the analytical procedure was assessed through calculation of recovery values. ExperimentalPlant Material The samples were collected from the "cerrado" of the Mato Grosso do Sul State, Brazil. A voucher specimen was deposited in the herbarium CCBS/UFMS, in Campo Grande/MS, Brazil.Chemicals Spectroscopy-grade acetonitrile, hexane, acetone, chloroform and methanol were purchased from Tedia Company/U.S.A. Water was purified using a Milli-Q system (Millipore). Jatrophone were isolated and purified by chromatography and recrystallization in Chemical Laboratories of the Mato Grosso do Sul Federal University. The identity of the standard was confirmed by MS, IR, and 1 H-and 13 C-NMR and their purity evaluated with HPLC-UV was 97.7% (Fig. 2). The "cachaça" (a commercial product containing distillates of sugar-cane, sugar and water) had and alcohol content of 38%). Stock mixtures of standard were made from the individual solutions in water-acetonitrile (13 : 7) and used as external standards.TLC Condition The chloroform fractions and the jatrop...

Neuromuscular block induced by Bothrops moojeni snake venom and the effect of Jatropha elliptica starch

“…So far, research on Jatropha elliptica extracts or secondary metabolites ratifies the medicinal potential of this plant and validated its activity on some of the symptoms of ophidian poisoning (BRUM et al, 2006;LIMA et al, 2006;PESSOA et al, 1999;2007;FERREIRA-RODRIGUES et al, 2016). However, an ethnobotanical study also describes the use of powder (starch), obtained from the rhizomes of the plant, administered orally and in small amounts as a treatment for this type of poisoning (SANTOS et al, 2006), which still needs to be investigated.…”