Quantitative determination of phenothiazine derivatives in human plasma using monolithic silica solid-phase extraction tips and gas chromatography–mass spectrometry

A new method for quantitative determination of dimemorfan in human plasma using monolithic silica solid-phase extraction tips

“…This method will be a useful tool for screening and quantitative determination of dimemorfan in therapeutic drug monitoring, clinical toxicology, and forensic toxicology. (7) Trimeprazine (IS) 10.0 89 (6) a Values are mean percentage and relative standard deviation (RSD) in parentheses.…”

“…The extraction profile of dimemorfan was examined by plotting the analyte abundance from the plasma samples against the number of aspirating/dispensing cycles (5,10,15,20,25, and 30 cycles). Extraction of the compound reached equilibrium after 20 aspirating/dispensing cycles, and this number of aspirating/dispensing cycles was used in subsequent experiments giving an extraction time of approximately 1 min.…”

“…The small bed volume and sorbent mass within the MonoTip C 18 tip requires a low solvent volume, which gives a smaller eluted volume, faster evaporation of the eluate, cleaner extract solution, and higher throughput than conventional SPE methods [5]. We previously demonstrated that micropipette tip-based SPE using MonoTip C 18 tips is an effective approach for extraction of antihistamines, amphetamines, tricyclic antidepressants, phenothiazines, and dextromethorphan from human body fluids [6][7][8][9][10][11].…”

Validation of a Method to Detect Central Nervous System Acting Drugs in Blood by Gc/Ms. Application in Cases of Death Caused by Phenothiazines in the North of Chile

“…The sensitivity showed by our method using GC/MS was similar to that previously reported by using chromatographic methods. [15][16] Table 3 shows the results obtained for intra-assay and inter-assay precision calculations and selected ions used for qualification and quantification of all the analytes. Inter-day and intra-day precision were < 9.0% and <3.5% for all analytes.…”

Sensitive and Rapid Voltammetric Determination of Phenothiazine and Azaphenothiazine Derivatives in Pharmaceuticals Using a Boron-doped Diamond Electrode

“…Analytical application of these compounds is based on their reduction properties and the capacity to form colorful charge-transfer type complexes or ionassociation bonds. A literature review shows that the methods of determination of phenothiazine derivatives mainly include chromatographic [3][4][5][6][7][8][9] and spectrophotometric ones. [10][11][12][13][14][15] With the development of modern pharmaceutical chemistry, the need arises to develop new methods of determination using contemporary analytical techniques.…”