Quantitative determination of pravastatin and its metabolite 3α‐hydroxy pravastatin in plasma and urine of pregnant patients by LC‐MS/MS

Abstract

This report describes the development and validation of a chromatography/tandem mass spectrometry method for the quantitative determination of pravastatin and its metabolite (3α-hydroxy pravastatin) in plasma and urine of pregnant patients under treatment with pravastatin, as part of a clinical trial. The method includes a one-step sample preparation by liquid-liquid extraction. The extraction recovery of the analytes ranged between 93.8% and 99.5% in plasma. The lower limits of quantitation (LLOQ) of the analytes in plasma samples were 0.106 ng/mL for pravastatin and 0.105 ng/mL for 3α-hydroxy pravastatin; while in urine samples they were 19.7 ng/mL for pravastatin and 2.00 ng/mL for 3α-hydroxy pravastatin. The relative deviation of this method was < 10 % for intra-day and inter-day assays in plasma and urine samples, and the accuracy ranged between 97.2% and 106% in plasma, and 98.2% to 105% in urine. The method described in this report was successfully utilized for determining the pharmacokinetics (PK) of pravastatin in pregnant patients enrolled in a pilot clinical trial for prevention of preeclampsia.

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL−33/ST2 Signaling

“…Targeted metabolomics (PA measurement) was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) as described previously ( 33 ). Briefly, cecum samples (100 mg) were dissolved in 900 μL of ice-cold water and extracted via sonication in water for 10 min.…”

Role of Uptake Transporters OAT4, OATP2A1, and OATP1A2 in Human Placental Bio-disposition of Pravastatin

“…The unlabeled pravastatin was quantified using liquid chromatographymass spectrometry method (LC-MS/MS) following the method reported previously with modifications (Supplementary Methods). 36 For the uptake into AM and BM placental vesicles, the uptake data were normalized to the protein amount, and the net glutarate-dependent uptake was calculated as a difference in the radiolabaled substrate quantified in the glutarate-preloaded vesicles and nonpreloaded (control).…”

Method Development and Validation for the Determination of Pravastatin in Human Plasma by Lc-Ms/Ms

“…In addition to pravastatin, 3α-iso-pravastatin and 6-epi-pravastatin could also be quantified in some assays [15,16]. In this project we attempted to combine previous experience and expertise to create reliable, sensitive, high-throughput LC-MS/MS method to determine pravastatin in human plasma by separating the major isobaric metabolites.…”