Quantitative determination of the enantiomers of methadone and its metabolite (EDDP) in human saliva by enantioselective liquid chromatography with mass spectrometric detection

Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and alprazolam in human plasma by liquid chromatography–electrospray ionization mass spectrometry

“…The LLOQ founded for MTD and/or EDDP were according with other in the refs. [22][23][24][25]. We found a wide calibration range that is especially useful in forensic cases.…”

Confirmatory analysis for drugs of abuse in plasma and urine by high-performance liquid chromatography?tandem mass spectrometry with respect to criteria for compound identification

“…Even though validated LC/MS methods for determination of drugs of abuse have been published, e.g. for determination of opioids in biological fluids [6][7][8][9], methadone in saliva [10], cocaine in urine [11], cocaine in several matrices [12], opiates and cocaine in meconium [13] and opioids, cocaine and metabolites in urine [14], none of the authors has followed the recommendations concerning the necessity to use at least two MRM transition for compound identification and/or they did not take into consideration the relative intensities of diagnostic ions or transitions. Only one study describes the use of quantifiers and qualifiers for confirmation of cocaine and benzoylecgonine in urine [15] and discusses the quality control requirements established by the Federal Department of Health and Human Services [16].…”

Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, 2-ethyl-5-methyl-3,3-diphenylpyraline and methadol in meconium by liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry

“…Some methods also have achieved high pg/ml sensitivity when quantifying the R and S enantiomers of methadone and EDDP [7][8][9][10][11]. However, meconium, a much more complex matrix due to the presence of bile acids and other excretory products, poses a difficult analytical challenge.…”