Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and alprazolam in human plasma by liquid chromatography–electrospray ionization mass spectrometry

Quintela, Óscar; López, Patricia; Bermejo, Ana María; López-Rivadulla, Manuel

doi:10.1016/j.jchromb.2006.02.013

Cited by 24 publications

(20 citation statements)

References 23 publications

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“…Furthermore, the flow rate has to be decreased from 0.4 mL min 1 to 0.3 mL min 1 due to high column pressure. Previous experiments labeled anhydrous diethyl ether to be a proper extracting agent and the addition of 50 L 0.1 M aqueous sodium hydroxide, as well as the increase of the extraction time to 2 min, further improved the extraction outcome [10,16].…”

Section: Methods Optimizationmentioning

confidence: 99%

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Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry

et al. 2010

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A new liquid chromatography-mass spectrometry method for the determination of carbamazepine, clonazepam, alprazolam, estazolam and phenytoin in human plasma has been developed by using diazepam as an internal standard. Chromatographic separation was performed on a Zorbax SB-C18 column (30 mm × 2.1 mm, 3.5 m) with a mobile phase consisting of methanol and aqueous 25 mM ammonium acetate using gradient elution. A diethyl ether extraction method was used for the extraction of five anti-epilepsy drugs. The final extract was injected for analysis by LC-MS/MS. The method was validated within the concentration range of 50-5000 ng mL 1 for five anti-epilepsy drugs. The precision of the assay (RSD%) was less than 10% at all concentration levels within the tested range. The method recoveries for all samples were more than 90%. The results indicate that the method is specific, sensitive and accurate, and suitable to study the pharmacokinetics, to adjust the dosage for individual administration, and to monitor the drug-concentration and drug abuse of the five anti-epilepsy drugs.liquid chromatography-mass spectrometry, anti-epilepsy drugs, plasma concentration

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Section: Methods Optimizationmentioning

confidence: 99%

“…However, most of them are limited to the quantification of one single AED and its major metabolites [8][9][10][11]. The reported methods include MECK [3], HPLC [8,12] and gas chromatography [13].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry

et al. 2010

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“…27,29,30 which are used for the determination of low therapeutic doses of benzodiazepines in human whole blood and plasma. According to the LODs from the literature shown in Table 3, our assay using the described Bond Elut Certify method of choice allows the detection and quantitation of flunitrazepam, nitrazepam, clonazepam, and alprazolam at subtherapeutic and toxic levels.…”

Section: Compoundmentioning

confidence: 99%

“…21 Another advantage is that one gets higher sensitivity (LOD) than other published studies using specific and sensitive detectors such as mass spectrometry. 25,[27][28][29][30]32 The combination of gas chromatography detectors presented here offers several possibilities for the routine analysis of benzodiazepines because they are compounds containing nitrogen atoms that can be located with the NPD and m-ECD for the presence the electronegative groups. Tables 1 and 2 show linearity, signal to noise ratio (S/N), relative standard deviation (RSD), limit of detection (LOD), limit of quantitation (LOQ), and solid-phase extraction efficiency in whole blood and human plasma with dual detection, m-ECD, and NPD of each drug.…”

mentioning

confidence: 99%

DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA

Bravo

Lobos

Venegas³

et al. 2010

J. Chil. Chem. Soc.

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Four benzodiazepines flunitrazepam, nitrazepam, clonazepam, and alprazolam, have been analyzed from biological matrices by dual column gas chromatography using a nitrogen-phosphorus detector (NPD) and a micro-electron capture detector (m-ECD). The recoveries of the four benzodiazepines spiked into human whole blood and plasma were 88.8 -97.9 and 90.7 -97.5%, respectively. The regression equations for the four benzodiazepines showed excellent linearity. The detection limits (LODs) were 0.14 -0.95 ng/mL for whole blood and 0.13 -0.93 ng/mL for plasma. The method is simple and sensitive for the determination of benzodiazepines in human whole blood and plasma, and may be useful in forensic science practice.

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“…It is rapidly absorbed and has a bioavailability of around 80-100% [4][2]. This molecule can be determined by a number of different methods such as: UV-VIS [5], TLC [6], GC-MS [7], LC-EI-MS [8][9], LC-MS-MS [1], HPLC-TOF/MS [10] in different materials such as: pharmaceutical formulations [5], human plasma [8][9], oral fluids [10], hair [7], blood [11], urine [2], etc. The stability of pharmaceutical molecules is a matter of great worry as it influences the safety and efficacy of the drug product [12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Short Term Photostability of Alprazolam Drug

Berisha¹,

Baxhaku²,

Sheqerxhiu³

et al. 2017

RadJ

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Abstract. Alprazolam is a widely prescribed sedative and antidepressant benzodiazepine (BDZ) drug which belongs to the class of anxiolytic, sedative and hypnotic anticonvulsants. It is believed that alprazolam (ALP) is more fairly safe and it rapidly reduces the symptoms of anxiety through the control of the central nervous system (CNS) excitability by a selective and potent enhancement of inhibitory gammaamino butyric acid (GABA) mediated neurotransmission. Accelerated photochemical stability of alprazolam was performed under several conditions. The study involved the evaluation of the photostability of Alprazolam in active mater, in PVC/Aluminum blister packet (BPT) or without blister packet tablets (WBPT). The evaluation of the stability was performed using special stability test chambers at 60% controlled humidity, temperature and UV irradiation. The High-Performance Liquid Chromatography (HPLC) was used to analyze the content of the Alprazolam before and after UV irradiation. The obtained results for the short term photostability study do not show any significant degradation of this molecule by the UV irradiation in active mater and this holds true for the BPT or WBPT, independent of the Alprazolam content (0.25 or 0.50 mg).

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Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and alprazolam in human plasma by liquid chromatography–electrospray ionization mass spectrometry

Cited by 24 publications

References 23 publications

Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry

Simultaneous determination of five anti-epilepsy drugs in human plasma using liquid chromatography-mass spectrometry

DEVELOPMENT AND VALIDATION OF A GC-NPD/micro-ECD METHOD USING DUAL COLUMN FOR THE DETERMINATION OF BENZODIAZEPINE IN HUMAN WHOLE BLOOD AND PLASMA

Evaluation of the Short Term Photostability of Alprazolam Drug

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