Abstract. Alprazolam is a widely prescribed sedative and antidepressant benzodiazepine (BDZ) drug which belongs to the class of anxiolytic, sedative and hypnotic anticonvulsants. It is believed that alprazolam (ALP) is more fairly safe and it rapidly reduces the symptoms of anxiety through the control of the central nervous system (CNS) excitability by a selective and potent enhancement of inhibitory gammaamino butyric acid (GABA) mediated neurotransmission. Accelerated photochemical stability of alprazolam was performed under several conditions. The study involved the evaluation of the photostability of Alprazolam in active mater, in PVC/Aluminum blister packet (BPT) or without blister packet tablets (WBPT). The evaluation of the stability was performed using special stability test chambers at 60% controlled humidity, temperature and UV irradiation. The High-Performance Liquid Chromatography (HPLC) was used to analyze the content of the Alprazolam before and after UV irradiation. The obtained results for the short term photostability study do not show any significant degradation of this molecule by the UV irradiation in active mater and this holds true for the BPT or WBPT, independent of the Alprazolam content (0.25 or 0.50 mg).