Quantitative determination of the topological propensities of amyloidogenic peptides

Shi, Yuan; Stouten, Pieter F. W.; Pillalamarri, Nirmala; Barile, Lauren; Rosal, Ramon; Teichberg, Saul; Bu, Zimei; Callaway, David J.E.

doi:10.1016/j.bpc.2005.09.015

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…More sophisticated and accurate schemes could be formulated by including structural effects or energetics derived from other experimental techniques (80). The approaches described above do not take into account interactions between b-sheet layers, which have been elucidated experimentally in Ab 1-40 fibrils (81) and GNNQQNY fibrils (30), but not in the fibrils discussed above.…”

Section: Theoretical Predictionsmentioning

confidence: 99%

Molecular Alignment within β-Sheets in Aβ14-23 Fibrils: Solid-State NMR Experiments and Theoretical Predictions

Shi

Callaway

et al. 2007

Biophysical Journal

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We report investigations of the molecular structure of amyloid fibrils formed by residues 14-23 of the b-amyloid peptide associated with Alzheimer's disease (Ab 14-23), using solid-state nuclear magnetic resonance (NMR) techniques in conjunction with electron microscopy and atomic force microscopy. The NMR measurements, which include two-dimensional proton-mediated 13 C-13 C exchange and two-dimensional relayed proton-mediated 13 C-13 C exchange spectra, show that Ab 14-23 fibrils contain antiparallel b-sheets with a registry of backbone hydrogen bonds that aligns residue 171k of each peptide molecule with residue 22ÿk of neighboring molecules in the same b-sheet. We compare these results, as well as previously reported experimental results for fibrils formed by other b-amyloid fragments, with theoretical predictions of molecular alignment based on databases of residue-specific alignments in antiparallel b-sheets in known protein structures. While the theoretical predictions are not in exact agreement with the experimental results, they facilitate the design of experiments by suggesting a small number of plausible alignments that are readily distinguished by solid-state NMR.

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Section: Theoretical Predictionsmentioning

confidence: 99%

Molecular Alignment within β-Sheets in Aβ14-23 Fibrils: Solid-State NMR Experiments and Theoretical Predictions

Shi

Callaway

et al. 2007

Biophysical Journal

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“…(i) According to NMR studies, Aβ 14−23 fragment assumes parallel β-sheet topology when incorporated in the polymerizing Aβ [8], although inherently anti-parallel β sheet topology is preferred for this fragment. In a study reported by Shi et al [120], the FRET method was used in exploration of the mentioned topology shift that occurs on the Aβ 14−23 fragment when incorporated in longer fragments. Aβ 14−23 fragment is referring to a fragment of Aβ between amino acids 14 and 23.…”

Section: Single Molecule Fluorescence Microscopymentioning

confidence: 99%

“…In the studies of Shi et al FRET was also used to reveal the role of specific amino acids in the aggregation of Aβ. This knowledge could help with the Aβ anti-aggregation drug discoveries [120]. (ii) The FRET technique, in combination with a double immunofluorescence approach, was used by Kinoshita et al in order to investigate the subcellular localization of AβPP and its interactions with human AβPP cleaving enzyme (known as β-secretase, or BACE) [60].…”

Section: Single Molecule Fluorescence Microscopymentioning

confidence: 99%

Nanotechnology Solutions for Alzheimer's Disease: Advances in Research Tools, Diagnostic Methods and Therapeutic Agents

Nazem

Mansoori

2008

JAD

122

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A century of research has passed since the discovery and definition of Alzheimer's disease (AD), the primary common dementing disorder worldwide. However, AD lacks definite diagnostic approaches and effective cure at the present. Moreover, the currently available diagnostic tools are not sufficient for an early screening of AD in order to start preventive approaches. Recently the emerging field of nanotechnology has promised new techniques to solve some of the AD challenges. Nanotechnology refers to the techniques of designing and manufacturing nanosize (1-100 nm) structures through controlled positional and/or self-assembly of atoms and molecules. In this report, we present the promises that nanotechnology brings in research on the AD diagnosis and therapy. They include its potential for the better understanding of the AD root cause molecular mechanisms, AD's early diagnoses, and effective treatment. The advances in AD research offered by the atomic force microscopy, single molecule fluorescence microscopy and NanoSIMS microscopy are examined here. In addition, the recently proposed applications of nanotechnology for the early diagnosis of AD including bio-barcode assay, localized surface plasmon resonance nanosensor, quantum dot and nanomechanical cantilever arrays are analyzed. Applications of nanotechnology in AD therapy including neuroprotections against oxidative stress and anti-amyloid therapeutics, neuroregeneration and drug delivery beyond the blood brain barrier (BBB) are discussed and analyzed. All of these applications could improve the treatment approach of AD and other neurodegenerative diseases. The complete cure of AD may become feasible by a combination of nanotechnology and some other novel approaches, like stem cell technology.

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“…

Nanofibers and nanorods have been encountered in natural biological systems, primarily amyloid peptides and proteins, [1][2][3][4][5][6] but have been also produced through diverse synthetic routes employing metals and semiconductors, [7,8] organic [9,10] and inorganic substances. [11] In general, two fundamental aspects govern nanostructure formation and morphology.

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mentioning

confidence: 99%

Lipid‐Modulated Pharmacophore Nanorods Assembled at the Air/Water Interface

Philosof-Mazor¹,

Volinsky²,

Jopp³

et al. 2009

ChemPhysChem

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Quantitative determination of the topological propensities of amyloidogenic peptides

Cited by 7 publications

References 35 publications

Molecular Alignment within β-Sheets in Aβ14-23 Fibrils: Solid-State NMR Experiments and Theoretical Predictions

Molecular Alignment within β-Sheets in Aβ14-23 Fibrils: Solid-State NMR Experiments and Theoretical Predictions

Nanotechnology Solutions for Alzheimer's Disease: Advances in Research Tools, Diagnostic Methods and Therapeutic Agents

Lipid‐Modulated Pharmacophore Nanorods Assembled at the Air/Water Interface

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