Quantitative Determination of Theophylline by an Automated Chemiluminescent Immunoassay in Serum and Plasma

Malliaros, David P.; Wong, Shan S.; Wu, Alan H.B.; Campbell, J.; Leonard, H; Houser, S.; Berg, Michael; Gornet, Terrie G.; Brown, Cindy; Feng, Yue

doi:10.1097/00007691-199704000-00019

Cited by 6 publications

(1 citation statement)

References 14 publications

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“…Among the reported methods for the determination of theophylline in plasma, such as fluorescent polarized immunoassay (Rojanasthien et al, 2001), chemiluminescent immunoassay (Malliaros et al, 1997), HPLC (Schrieber-Deturmeny and Bruguerolle, 1996;Umemura et al, 1998;Kizu et al, 1999), LC-MS (Watson et al, 2001;Kanazawa et al, 2000), HPLC is the most common used one. In this study, an HPLC method was used to determine the pharmacokinetics of TGS SR tablets with the new asymmetric regimen, which was previously developed and validated in our group and used to study the single-and multiple-dose pharmacokinetics of TSG SR tablets (Yao et al, 2000).…”

Section: Methods Development and Validationmentioning

confidence: 99%

Pharmacokinetics of a new sustained‐release formulation of theophylline sodium glycerinate in healthy subjects with a new asymmetric dosage regimen

Wang

Zhong

et al. 2003

Biomedical Chromatography

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Pharmacokinetics of a new sustained-release formulation of theophylline sodium glycerinate in healthy subjects was studied. In this study, a new asymmetric dosage regimen was presented to achieve a better accordance with the chronotherapy of asthma. Each of 10 subjects was administered one tablet (equivalent to 0.1 g anhydrous theophylline) in the morning and four tablets in the evening for a consecutive 6 days and blood samples were collected at the predetermined time and analyzed by a validated HPLC method. This new regimen produced a steady and effective level of theophylline in plasma for the whole day, especially in the evening. A lower dose in the morning could reach the effective level (C(min)4.97 +/- 1.60 microg/mL and C(max)10.68 +/- 1.80 micro g/mL over the a.m. dosing interval) and a higher dose in the evening did not result in toxic levels but led to a reasonable concentration range (C(max)9.72 +/- 1.56 microg/mL over p.m. dosing interval), which could maintain a higher plasma theophylline concentration without the risk of serious adverse events and control asthmatic symptoms probably occurring during the night or early in the morning. The results suggested that the proposed asymmetric regimen was necessary, practicable and safe for twice daily sustained-release tablets of theophylline sodium glycerinate and also provides the basis for the clinical dosage regimen of other theophylline formulated products.

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