Quantitative determination of tocopherols in single seeds of rapeseed (Brassica napus L.)

Goffman, Fernando D.; Velasco, Leonardo; Thies, W.

doi:10.1002/(sici)1521-4133(199904)101:4<142::aid-lipi142>3.0.co;2-j

Cited by 41 publications

(26 citation statements)

References 10 publications

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“…Tocopherol analyses: Tocopherol analyses were conducted using the analytical procedure reported by Goffman et al. (1999) with slight modifications.…”

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confidence: 99%

Identification and genetic characterization of a safflower mutant with a modified tocopherol profile

2005

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Safflower (Carthamus tinctorius L.) seeds have a tocopherol fraction dominated by a-tocopherol, which accounts for more than 95% of the tocopherols. a-Tocopherol exerts a high vitamin E activity, but a low in vitro antioxidant action. For non-food applications, replacement of high a-tocopherol by c-tocopherol is preferred. Because of the limited variability found in germplasm of cultivated safflower, the objective of the present research was to search for variability for tocopherol profile in germplasm of wild safflower species. Bulk seed samples of 77 accessions from six species were analysed for total tocopherol content and profile. One accession of C. oxyacanthus showed increased c-tocopherol content (36%). Single-seed analysis showed the existence of a bimodal distribution, which included high a-tocopherol (>90%) and high c-tocopherol (HGT) seeds (>85%). Plants from the HGT seeds bred true for the trait and showed introgression of cultivated safflower, which allowed a rapid selection for cultivated safflower traits. Genetic analysis revealed that the HGT trait was controlled by partially recessive alleles at a single locus Tph1, which will facilitate the transference of the novel trait to diverse safflower germplasms.

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“…Tocopherol analyses: Tocopherol analyses were conducted using the analytical procedure reported by Goffman et al. (1999) with slight modifications.…”

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confidence: 99%

Identification and genetic characterization of a safflower mutant with a modified tocopherol profile

2005

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“…Six randomly selected seeds from each plant were husked, weighed, and crushed as fine as possible with a stainless steel rod. Tocopherol extraction, separation by high‐performance liquid chromatography, and quantification was done following the procedures described by Goffman et al (1999) using a fluorescence detector (Waters 474, Waters Corporation) at 295 nm excitation and 330 nm emission and iso‐octane:ert‐butylmethylether (94:6) as eluent at an isocratic flow rate of 0.8 mL min – 1 . Chromatographic separation of the tocopherols was performed on a LiChrospher 100 diol column (250 by 2 mm i.d.)…”

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confidence: 99%

Environmental Stability of Contrasting Seed Tocopherol Profiles in Sunflower

Velasco

Fernández‐Martínez

2012

Crop Science

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“…M 1 plants were self-pollinated, using paper bags to prevent uncontrolled pollination. A total of 1080 M 1 plants were harvested.Six M 2 half-seeds per M 1 plant were analysed for tocopherol profile following the method of Goffman et al (1999). Half U. S.…”

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confidence: 99%

“…Six M 2 half-seeds per M 1 plant were analysed for tocopherol profile following the method of Goffman et al (1999). Half U. S.…”

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confidence: 99%

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Novel variation for the tocopherol profile in a sunflower created by mutagenesis and recombination

2004

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a-Tocopherol is the main tocopherol in sunflower seeds (>90%). Because it exerts a weak antioxidant action in vitro, its partial replacement by other tocopherols is an important breeding objective in this crop. The objective of this research was to develop novel tocopherol profiles in sunflower through mutagenesis and genetic recombination. Seeds of four ÔPeredovikÕ accessions were used for chemical mutagenesis with ethyl methane sulfonate (EMS). Singleseed screening in the M 2 generation resulted in two M 2 seeds, derived from different M 1 plants, with increased c-tocopherol contents of 19.2% and 96.7%, respectively. M 3 progeny from the M 2 seed with the 96.7% content bred true for high c-tocopherol content, containing more than 90% c-tocopherol. M 3 progeny from the M 2 seed with only 19.2% c-tocopherol segregated in a range from 0 to 84.6%. Selection for high c-tocopherol content produced an M 4 : 5 line, designated IAST-1, with a stable high concentration of c-tocopherol. Crosses between IAST-1 and T589, with an increased b-tocopherol content, produced F 2 segregants with transgressive levels of up to 77% b-tocopherol or up to 68% d-tocopherol. Both novel tocopherol profiles were confirmed in the F 3 generation.Tocopherols are the most important antioxidants in sunflower seeds. They exert an antioxidant protective action both in biological systems (in vivo or by vitamin E activity) as well as in oils, fats and food (in vitro antioxidant activity) (Kamal-Eldin and Appelqvist 1996). The tocopherols occur as a family of four derivatives named a-, b-, c-, and d-tocopherol, which differ in their in vitro and in vivo antioxidant efficiency. a-Tocopherol has a maximum vitamin E activity, but its in vitro activity is relatively low. Conversely, b-, c-, and d-tocopherol possess a lower vitamin E value, but they are better in vitro antioxidants than a-tocopherol (Pongracz et al. 1995).Sunflower seeds mainly contain a-tocopherol, which accounts for more than 90% of the total tocopherols. b-and c-tocopherol can be present in sunflower seeds, usually in amounts below 5% of the total tocopherols (Demurin 1993, Velasco et al. 2002. The modification of the tocopherol profile through a partial substitution of a-tocopherol, with its weak in vitro antioxidant action, by other tocopherol derivatives is an important goal for developing sunflower oil with improved oxidative stability (Sˇkoric´1992). Breeding efforts in this direction during the past decade have led to the development and characterization of several sources of modified tocopherol profiles in sunflower. Through a strategy based on evaluation of germplasm of wild and cultivated sunflower, Demurin (1993) and Demurin et al. (1996) isolated two lines with contrasting tocopherol profiles. The line LG-15, with increased concentration of b-tocopherol (50% of the total tocopherols), was developed from the open-pollinated variety ÔVNIIMK 8931Õ, whereas the line LG-17, with increased concentration of c-tocopherol (95% of the total tocopherols), was isolated from a germplasm...

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Quantitative determination of tocopherols in single seeds of rapeseed (Brassica napus L.)

Cited by 41 publications

References 10 publications

Identification and genetic characterization of a safflower mutant with a modified tocopherol profile

Identification and genetic characterization of a safflower mutant with a modified tocopherol profile

Environmental Stability of Contrasting Seed Tocopherol Profiles in Sunflower

Novel variation for the tocopherol profile in a sunflower created by mutagenesis and recombination

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