A procedure is described which allows the isolation of ca. 1 g Sinigrin (K‐salt) or glucotropaeolin (tetramethylammonia‐(TMA)‐salt) from seeds of Brassica nigra and Lepidium sativum, respectively within one day as crystalline substances. Both compounds possess excellent long term stabilities. In contrast to sinigrin the TMA salt of glucotropaeolin does not contain water of crystallization.

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A method is described which allows a fast determination of the content of glucosinolates in cruciferous seeds. Basically the reaction between glucosinolates and tetrachloropalladate (II) is used. For the quantitative measurement of the resulting coordination complexes an 8‐channel photometer is recommended. Furthermore an analytical grist‐mill has been constructed which allows a fast preparation of the samples.

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