Quantitative Determination of Triperpene Saponins and Alkenated-Phenolics from Labisia pumila by LC-UV/ELSD Method and Confirmation by LC-ESI-TOF

Avula, B; Wang, YH; Ali, Z; Smillie, TJ; Khan, IA

doi:10.1055/s-0030-1251787

Cited by 12 publications

(14 citation statements)

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“…including the compound ardisiacrispin A which were thought to interact with the estrogen receptors (Avula et al, 2010). LP was also capable of exhibiting antioxidative properties (Mohamad et al, 2009) which may be responsible for its bone protective actions.…”

Section: Discussionmentioning

confidence: 99%

Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Fathilah

Shuid

Mohamed

et al. 2012

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Fathilah

Shuid

Mohamed

et al. 2012

Journal of Ethnopharmacology

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“…Traditionally, L. pumila extract is prepared by boiling the roots, leaves or the whole plant in water and the extract is taken orally and used to accelerate labor, shrink the uterus, improve menstrual cycle and for weight loss [12]. The antioxidant activity of the aqueous L. pumila extract has been reported as providing significant protection to human dermal fibroblasts and from cell damage caused by UV irradiation [13], most likely due to the presence of secondary metabolites, i.e. , flavonoids and phenolics [14].…”

Section: Introductionmentioning

confidence: 99%

Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) Exposed to Potassium Fertilization under Greenhouse Conditions

Ibrahim

Jaafar

Karimi

et al. 2012

IJMS

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A randomized complete block design was used to characterize the relationship between production of total phenolics, flavonoids, ascorbic acid, carbohydrate content, leaf gas exchange, phenylalanine ammonia-lyase (PAL), soluble protein, invertase and antioxidant enzyme activities (ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD) in Labisia pumila Benth var. alata under four levels of potassium fertilization experiments (0, 90, 180 and 270 kg K/ha) conducted for 12 weeks. It was found that the production of total phenolics, flavonoids, ascorbic acid and carbohydrate content was affected by the interaction between potassium fertilization and plant parts. As the potassium fertilization levels increased from 0 to 270 kg K/ha, the production of soluble protein and PAL activity increased steadily. At the highest potassium fertilization (270 kg K/ha) L. pumila exhibited significantly higher net photosynthesis (A), stomatal conductance (gs), intercellular CO2 (Ci), apparent quantum yield (ξ) and lower dark respiration rates (Rd), compared to the other treatments. It was found that the production of total phenolics, flavonoids and ascorbic acid are also higher under 270 kg K/ha compared to 180, 90 and 0 kg K/ha. Furthermore, from the present study, the invertase activity was also found to be higher in 270 kg K/ha treatment. The antioxidant enzyme activities (APX, CAT and SOD) were lower under high potassium fertilization (270 kg K/ha) and have a significant negative correlation with total phenolics and flavonoid production. From this study, it was observed that the up-regulation of leaf gas exchange and downregulation of APX, CAT and SOD activities under high supplementation of potassium fertilizer enhanced the carbohydrate content that simultaneously increased the production of L. pumila secondary metabolites, thus increasing the health promoting effects of this plant.

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“…No current UPLC‐MS/MS method exists for the determination of ardisiacrispin A to characterize pharmacokinetic properties in rats. There have been few studies reported for separation and determination of ardisiacrispins A from Ardisia species by HPLC and UPLC‐MS/MS methods (Avula et al, ; Ma et al, ). Avula et al developed an LC‐UV/ELSD method for quantitative determination of triperpene saponins and alkenated phenolics from Labisia pumila , with the run time of 45 min and an LLOQ of 25 ng/mL.…”

Section: Resultsmentioning

confidence: 99%

“…There are some recent methods developed for the determination of triterpenes (Li et al, , b; Ma et al, ), but there have been few studies reported on the separation and determination of ardisiacrispins A from Ardisia species by HPLC and UPLC‐MS/MS methods (Avula, Wang, Ali, Smillie, & Khan, ; Ma et al, ). However, these methods are limited by long chromatographic run time and insufficient sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC–MS/MS

Fang

Bao

Wang

et al. 2016

Biomedical Chromatography

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In this work, a sensitive and selective UPLC-MS/MS method for determination of ardisiacrispin A in rat plasma was developed. Cyasterone used as an internal standard (IS) and protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C column (2.1 × 100 mm, 1.7 μm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 1083.5 → 407.1 for ardisiacrispin A and m/z 521.3 → 485.2 for IS. Calibration plots were linear throughout the range 5-2000 ng/mL for ardisiacrispin A in rat plasma. Mean recoveries of ardisiacrispin A in rat plasma ranged from 80.4 to 92.6%. The values of RSD of intra- and inter-day precision were both <11%. The accuracy of the method was between 97.3 and 105.6%. The method was successfully applied to pharmacokinetic study of ardisiacrispin A after intravenous administration in rats.

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Quantitative Determination of Triperpene Saponins and Alkenated-Phenolics from Labisia pumila by LC-UV/ELSD Method and Confirmation by LC-ESI-TOF

Cited by 12 publications

References 0 publications

Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) Exposed to Potassium Fertilization under Greenhouse Conditions

Pharmacokinetic study of ardisiacrispin A in rat plasma after intravenous administration by UPLC–MS/MS

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