Quantitative differences in tissue surface tension influence zebrafish germ layer positioning

Schötz, Eva-Maria; Burdine, Rebecca D.; Jülicher, Frank; Steinberg, Malcolm S.; Heisenberg, Carl‐Philipp; Foty, Ramsey A.

doi:10.2976/1.2834817

Cited by 145 publications

(167 citation statements)

References 48 publications

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“…Figure 1e shows the log of the MSD as a function of log of the time for ectoderm and mesendoderm tissues. We find that D ¼ 0. rsif.royalsocietypublishing.org J R Soc Interface 10: 20130726 mesendoderm explants have previously been shown [1] to differ by a factor of 2 in their tissue surface tensions, thus the observed difference in the diffusion constant is not surprising as we would expect cells in more adhesive tissues to move less. The slope of this plot, which is a second dimensionless observable a, is nearly unity at long times for both tissues indicating that the nuclei movement is diffusive.…”

Section: Statistics Of Individual Cell Trajectoriesmentioning

confidence: 89%

“…no. 15517-022, Invitrogen) in E2-medium [1] to minimize motion. Imaging was carried out on a custom-built two-photon laser scanning microscope, constructed on an upright BX51 Olympus microscope (Tokyo, Japan).…”

Section: Two-photon Imaging and Cell Trackingmentioning

confidence: 99%

“…Maternal zygotic one-eyed pinhead (MZoep) and 50-100 pg of cyclops mRNA-injected wild-type zebrafish aggregates, corresponding to ectoderm and mesendoderm, respectively, were generated and fluorescently labelled as previously described [1,24,26].…”

Section: Explant Preparationmentioning

confidence: 99%

“…A quantitative description of the mechanical behaviour of groups of cells in biological tissues is critical for an understanding of many fundamental biological processes, including embryogenesis [1][2][3][4][5][6][7], wound healing [8,9], stem cell dynamics, regeneration [10,11] and tumourigenesis [12][13][14][15]. Previous work demonstrates that the macroscopic response of many tissues is viscoelastic [1], where the tissue behaves as an elastic solid over short timescales and a viscous fluid over long timescales.…”

Section: Introductionmentioning

confidence: 99%

“…Previous work demonstrates that the macroscopic response of many tissues is viscoelastic [1], where the tissue behaves as an elastic solid over short timescales and a viscous fluid over long timescales. The relaxation timescale, a material parameter that is different for different tissue types, characterizes how much time it takes for a tissue to crossover from elastic to viscous behaviour.…”

Section: Introductionmentioning

confidence: 99%

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Glassy dynamics in three-dimensional embryonic tissues

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et al. 2013

J. R. Soc. Interface.

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Many biological tissues are viscoelastic, behaving as elastic solids on short timescales and fluids on long timescales. This collective mechanical behaviour enables and helps to guide pattern formation and tissue layering. Here, we investigate the mechanical properties of three-dimensional tissue explants from zebrafish embryos by analysing individual cell tracks and macroscopic mechanical response. We find that the cell dynamics inside the tissue exhibit features of supercooled fluids, including subdiffusive trajectories and signatures of caging behaviour. We develop a minimal, three-parameter mechanical model for these dynamics, which we calibrate using only information about cell tracks. This model generates predictions about the macroscopic bulk response of the tissue (with no fit parameters) that are verified experimentally, providing a strong validation of the model. The best-fit model parameters indicate that although the tissue is fluid-like, it is close to a glass transition, suggesting that small changes to single-cell parameters could generate a significant change in the viscoelastic properties of the tissue. These results provide a robust framework for quantifying and modelling mechanically driven pattern formation in tissues.

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Section: Statistics Of Individual Cell Trajectoriesmentioning

confidence: 89%

Section: Two-photon Imaging and Cell Trackingmentioning

confidence: 99%

Section: Explant Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Glassy dynamics in three-dimensional embryonic tissues

Schötz

Lanio

Talbot

et al. 2013

J. R. Soc. Interface.

133

137

View full text Add to dashboard Cite

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Fibroblasts Promote Proliferation and Matrix Invasion of Breast Cancer Cells in Co‐Culture Models

Plaster

Singh

Tavana

2019

Advanced Therapeutics

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Fibroblasts are an abundant cell type in tumor microenvironments. Activated fibroblasts, known as carcinoma-associated fibroblasts (CAFs), interact with cancer cells through biochemical signaling and render cancer cells proliferative, invasive, and resistant to therapeutics. Targeting CAFs-cancer cells interactions offers a strategy to block cancer progression. 2D and 3D co-cultures of human mammary fibroblasts and triple negative breast cancer (TNBC) cells are used to investigate the impact of heterotypic cellular interactions on the proliferation of matrix invasion of TNBC cells. The resultsshow that fibroblasts secreting a chemokine, CXCL12, significantly enhance proliferation of TNBC cells expressing the chemokine receptor, CXCR4. Disrupting this interaction with a receptor antagonist normalizes cancer cell proliferation to that of a co-culture model lacking this signaling. When co-culture spheroids are embedded in collagen, fibroblasts producing CXCL12 promote collagen invasion of TNBC cells. Although co-cultures containing normal fibroblasts also lead to TNBC cell spreading into the matrix, a morphological analysis of cells and inhibition of chemokine-receptor signaling shows that this spreading is due to the incompatibility of fibroblasts and cancer cells leading to the segregation of the two cell types from the spheroid.

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Relating cell and tissue mechanics: Implications and applications

Jakab

Damon

Marga

et al. 2008

Developmental Dynamics

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The Differential Adhesion Hypothesis (DAH) posits that differences in adhesion provide the driving force for morphogenetic processes. A manifestation of differential adhesion is tissue liquidity and a measure for it is tissue surface tension. In terms of this property, DAH correctly predicts global developmental tissue patterns. However, it provides little information on how these patterns arise from the movement and shape changes of cells. We provide strong qualitative and quantitative support for tissue liquidity both in true developmental context and in vitro assays. We follow the movement and characteristic shape changes of individual cells in the course of specific tissue rearrangements leading to liquid-like configurations. Finally, we relate the measurable tissue-liquid properties to molecular entities, whose direct determination under realistic three-dimensional culture conditions is not possible. Our findings confirm the usefulness of tissue liquidity and provide the scientific underpinning for a novel tissue engineering technology. Developmental Dynamics 237:2438 -2449, 2008.

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Quantitative differences in tissue surface tension influence zebrafish germ layer positioning

Cited by 145 publications

References 48 publications

Glassy dynamics in three-dimensional embryonic tissues

Glassy dynamics in three-dimensional embryonic tissues

Fibroblasts Promote Proliferation and Matrix Invasion of Breast Cancer Cells in Co‐Culture Models

Relating cell and tissue mechanics: Implications and applications

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