Quantitative effects of allogeneic small-bowel transplantation of nutrient digestion and on body protein balance as determined in vivo in rats

Kreuzer, Michael; Kirchgeßner, M.; Ascherl, R.; Heinz, S.; J, Meier; Maderholz, H

doi:10.1007/bf00000039

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Therefore, knowledge of differences in susceptibility to cold preservation injury and subsequent reperfusion injury between the proximal and distal small bowel is important for avoiding primary mal-function. Many factors regarding intestinal function and enzymes have been reported as useful parameters for determining intestinal viability [4][5][6][7][8]. However, there is no single factor for distinguishing transplant tolerability between the jejunum and the ileum.…”

mentioning

confidence: 99%

Segmental Difference in Epithelial and Mucosal Barrier Functions between the Jejunum and the Ileum in Cold Storage Small Bowel Grafts in the Rat

Matsuno

2004

Kurume Med. J.

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The purpose of this study was to determine the differences in epithelial and mucosal barrier function between the jejunum and the ileum at transplantation. Rat small bowel was preserved in cold University of Wisconsin solution for 0, 12 or 24 hrs. Time-related segmental differences in the potential differences, at rest (rPD) and upon glucose stimulation (PDgs), and in the permeation rates of phenolsulfonphtalein (PSP) and polyethylene glycol 4000 (PEG) as non-absorbable markers were studied after a 20-min reoxygenation period following cold preservation. Time-related histologic damage during cold preservation was also evaluated. After 24 hrs of preservation, when the villi were denuded both in jejunum and ileum, the rPD (1.4 +/- 0.3 mV/cm2) and PDgs (1.4 +/- 0.3 mV/cm2) of the jejunum were significantly lower than those of the ileum (1.9 +/- 0.3 mV/cm2 and 2.1 +/- 0.3 mV/cm2, respectively). A difference in PEG permeation rates occurred after 24 hrs of preservation. The permeation rate of PSP in the jejunum was 10.5 +/- 1.0%, which was significantly higher than that in the ileum (8.6 +/- 1.0%) after 12 of preservation, when subepithelial edema occurred both in the jejunum and ileum. Our functional study demonstrated that, in the rat small bowel, the ileumis more resistant than the jejunum to cold preservation.

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confidence: 99%

Segmental Difference in Epithelial and Mucosal Barrier Functions between the Jejunum and the Ileum in Cold Storage Small Bowel Grafts in the Rat

Matsuno

2004

Kurume Med. J.

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“…21 As it was not easy to obtain histological specimens after the stomas were closed, we assessed changes in the lipid absorption of the grafts. 11,12,20 Fecal fat excretion in the allo-R-SBT rats was significantly increased 14 days after the first grafting, when the grafts seemed to be normal in the H-SBT animals. Autopsy of the allo-R-SBT rats also confirmed no lymphocyte infiltration in the grafted intestine.…”

Section: Discussionmentioning

confidence: 97%

“…Changes in the absorptive function of an ileal graft are considered to be a more useful parameter for the detection of chronic rejection than histological examination, as reported by us and others. [11][12][13] Histological changes in allo-intestinal grafts appear earlier after heterotopic than orthotopic transplantation. 14,15 Accordingly, we examined changes in fecal fat excretion after orthotopic segmental SBT in rats, comparing these findings with histological findings in heterotopic grafts.…”

Section: Discussionmentioning

confidence: 99%

Changes in fecal fat excretion from allo-intestine transplanted rats after discontinuance of immunosuppressive agents

Saito

Kobayashi

Ogino

et al. 1999

Journal of Gastroenterology

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We have previously shown in a rat model of segmental small bowel transplantation, that an ileal graft was more favorable for lipid absorption than a jejunal graft. In the present study, we investigated changes in the lipid absorption of allo-ileal grafts after the withdrawal of an immunosuppressant (methyldeoxyspergualin; given for 1 or 2 weeks at 2.5mg/kg per day) and compared the findings with the results of histological examinations. Heterotopic segmental small bowel transplantation (SBT) was performed using cuff anastomoses in the inbred rat strain combination DA (RT1a) and LEW (RT1). Orthotopic SBT was performed as reconstruction SBT. Reconstruction after resection of the original intestinal tract of the recipient was performed on the seventh day after heterotopic segmental ileal grafting. For the histological evaluation of graft rejection, we performed biopsies in the heterotopic model and autopsies in the orthotopic model. Fecal fat excretion from the orthotopically reconstructed recipients was measured by Folch's method. One- and 2-week use of methyldeoxyspergualin allowed the heterotopic allografts to remain intact histologically for 16.8+/-5.6 and 25.3+/-3.3 (mean+/-SD) days, respectively, whereas the grafts were rejected at 7.3+/-1.1 days when the immunosuppressive drug was not used. All the recipients in the orthotopic group had died by 19 days after reconstruction. After discontinuance of the immunosuppressive agent, fat excretion in feces in the surviving orthotopic grafted rats had increased markedly prior to histologically assessed heterotopic graft rejection. Evaluation of fecal lipid excretion may be a useful parameter for detecting graft rejection in rats.

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“…They did not perform any histological investigation of the intestinal grafts though, while in our experiments all of the graft biopsies were normal. Kreuzer et al [16] have reported impaired fat and protein absorption of orthotopically transplanted halflength intestines in CyA-treated allografted rats. The impaired absorption was thought to be attributed to the short intestinal graft.…”

Section: Discussionmentioning

confidence: 99%

Integrated intestinal capacity and nutritional status following small bowel transplantation

1997

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Successful small bowel transplantation requires normal functional capacity of the graft and unaltered metabolism of the host. Weight gain and wet weight of muscle groups and intra-abdominal fat pads were compared between transplanted, sham-operated, short bowel-operated, and normal rats that were fed either standard chow or fat-enriched (15 %) pellets. Weight gain and wet weight of muscle groups and fat pads for the control, transplanted, and sham-operated rats were identical, while short bowel animals showed reduced weight. Transplanted rats receiving fat-enriched food had lower wet weight of fat pads than control animals on the high-fat diet. We conclude that small bowel transplantation makes it possible to overcome the intestinal failure associated with short bowel syndrome, leading to overall normal weight gain and development of the recipient. However, altered fat metabolism, reflected in changed body composition, was observed in transplanted animals on the high-fat diet.

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Quantitative effects of allogeneic small-bowel transplantation of nutrient digestion and on body protein balance as determined in vivo in rats

Cited by 5 publications

References 31 publications

Segmental Difference in Epithelial and Mucosal Barrier Functions between the Jejunum and the Ileum in Cold Storage Small Bowel Grafts in the Rat

Segmental Difference in Epithelial and Mucosal Barrier Functions between the Jejunum and the Ileum in Cold Storage Small Bowel Grafts in the Rat

Changes in fecal fat excretion from allo-intestine transplanted rats after discontinuance of immunosuppressive agents

Integrated intestinal capacity and nutritional status following small bowel transplantation

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