1992
DOI: 10.1523/jneurosci.12-05-01977.1992
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Quantitative enzyme radioautography with 3H-Ro 41-1049 and 3H-Ro 19- 6327 in vitro: localization and abundance of MAO-A and MAO-B in rat CNS, peripheral organs, and human brain

Abstract: Monoamine oxidases A and B (MAO-A and MAO-B) oxidatively deaminate neurotransmitter and xenobiotic amines. Since the cellular localization of the isoenzymes in the CNS and peripheral organs determines to a large extent which substrate has access to which isoenzyme, knowledge of their tissue distribution and cellular localization is essential. Here we describe how reversible and selective inhibitors of MAO-A and MAO-B [Ro 41–1049 and Ro 19–6327 (lazabemide), respectively] can be used, as tritiated radioligands,… Show more

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Cited by 318 publications
(203 citation statements)
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“…In brain tissue, MAO-A level is highly correlated with MAO-A activity and MAO-A V T is primarily an index of MAO-A level (Saura et al, 1992). Elevated MAO-A activity is related to several important downstream effects, as MAO-A participates in the metabolism of serotonin, dopamine, and norepinephrine, produces the pro-oxidant hydrogen peroxide and influences the predisposition toward apoptosis (Fitzgerald et al, 2007;Ou et al, 2006b;Youdim et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In brain tissue, MAO-A level is highly correlated with MAO-A activity and MAO-A V T is primarily an index of MAO-A level (Saura et al, 1992). Elevated MAO-A activity is related to several important downstream effects, as MAO-A participates in the metabolism of serotonin, dopamine, and norepinephrine, produces the pro-oxidant hydrogen peroxide and influences the predisposition toward apoptosis (Fitzgerald et al, 2007;Ou et al, 2006b;Youdim et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
“…MAO-A level is highly correlated with MAO-A activity in brain (Nelson et al, 1979;Saura et al, 1992) and indices of MAO-A level in the prefrontal (PFC) and anterior cingulate cortex (ACC) were elevated by 25-40% across the three studies that investigated the most common, early onset MDD (Johnson et al, 2011;Meyer et al, 2006;Meyer et al, 2009). Elevated MAO-A level in these regions in MDE is consistent with other specific findings: MDE is a highstress state, and glucocorticoid administration increases transcription rate and catalytic activity of MAO-A in human neuroblastoma and glioblastoma cell lines (Ou et al, 2006a).…”
Section: Introductionmentioning
confidence: 99%
“…Clorgyline and pargyline are commercial MAO inhibitors and their IC 50 were included as references (24, 25 in Table 1). 12,13) As expected, nearly all compounds show good inhibitory activity toward MAO-A/B. As can be seen from Table 1, benzhydrylidene-prop-2-ynyl-amine (2, IC 50 ϭ 32 nM) and (3,4-dimethoxy-benzylidene)-prop-2-ynyl-amine (10, IC 50 ϭ14 nM) are the best inhibitors toward monoamine oxidase A and B respectively, while compound 1 (SIϭ58.96) is the most selective inhibitor toward MAO-A and compound 2 (SIϭ0.34) is the most selective inhibitor toward MAO-B.…”
Section: Resultsmentioning
confidence: 99%
“…For example, reduced mitochondrial respiration, an index of reduced mitochondrial functioning, occurs when MAO-A activity is increased (54), and AD is associated with reduced N-acetylaspartate levels, an index of reduced mitochondrial function (55,56). Also, in contrast to many other brain proteins, MAO-A is present homogenously throughout the cortex, including glial cells (6), and when MAO-A activity is greater, glial cell lines are predisposed toward apoptosis (24). MAO-A activity itself creates oxidative stress (4), and markers of oxidative damage to mitochondrial DNA have been reported in AD (51,57).…”
Section: Discussionmentioning
confidence: 99%
“…MAO-A metabolizes monoamines such as serotonin, norepinephrine, and dopamine, and levels of MAO-A in brain tissue show a strong, positive correlation with MAO-A activity (5,6). Previous investigations of MAO-A activity in postmortem brain of AD were primarily negative, with no change reported in prefrontal cortex, and a decrease reported in the hypothalamus and caudate (7,8).…”
mentioning
confidence: 98%