Quantitative Estimation and Antimicrobial Potential of Ethanol Extract of Durio Zibethinus Murr. Leaves

Chigurupati, Sridevi; Mohammad, Jahidul Islam; Vijayabalan, Shantini; Vaipuri, Narmatha Devi; Selvarajan, Kesavanarayanan Krishnan; Nemala, Appala Raju

doi:10.22159/ajpcr.2017.v10i9.19767

Cited by 12 publications

(13 citation statements)

References 9 publications

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“…A series of qualitative phytochemical tests on the extract used to identify the presence of constituent such as alkaloids, flavonoids, phenols, mucilage, tannins, gums, glycosides, non-reducing sugars, saponins, proteins, monosaccharides, steroids, amino acids, carbohydrates, and reducing sugars [8].…”

Section: Qualitative Phytochemical Testsmentioning

confidence: 99%

Screening Antimicrobial Potential for Malaysian Originated Tamarindus Indica Ethanolic Leaves Extract

Chigurupati

Yiik

Mohammad

et al. 2018

Asian J Pharm Clin Res

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Objective: The aim of the research is to study the antimicrobial potential of Tamarindus indica leaves extract (Malaysian origin). Methods:T. indica leaves extraction was carried out by maceration (TIME) and Soxhlet extraction methods (TISE). The phytochemical test was conducted for the confirmation of various phytoconstituents present in the leaves extract. Antimicrobial assays were carried out by minimum bactericidal concentration (MBC), minimum inhibitory concentration (MIC), and agar well diffusion method against ATCC bacterial strains.Results: Ethanolic extraction of T. indica leaves by maceration method showed better yield (70.38%) compared to Soxhlet extraction method (60.55%). The qualitative phytochemical test on TISE and TIME were confirmed the presence of alkaloids, flavonoids, phenols, mucilage, tannins, steroids, proteins, and carbohydrates. TIME was selected for antimicrobial studies, and it was found to have MBC value of 500 µg/mL and MIC value of 250 µg/mL among various test bacteria, and it was also found to exhibit good zone of inhibition against Gram-negative bacteria such as Escherichia coli and Neisseria gonorrhoeae.Conclusion: These findings deduced that T. indica leaves have various phytochemical constituents which could be responsible for their natural antibacterial activity especially against Gram-negative bacteria.

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Section: Qualitative Phytochemical Testsmentioning

confidence: 99%

Screening Antimicrobial Potential for Malaysian Originated Tamarindus Indica Ethanolic Leaves Extract

Chigurupati

Yiik

Mohammad

et al. 2018

Asian J Pharm Clin Res

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“…Phytochemical analysis was carried out for CILE as per the standard methods ( Majid et al, 2015 , Roopashree et al, 2008 ). A series of phytochemical tests on the extract used to identify the presence of constituents such as saponin, flavonoid, gum, tannin, glycoside, protein, phenol, starch and carbohydrate ( Chigurupati et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Pharmacological and pharmacognostical valuation of Canna indica leaves extract by quantifying safety profile and neuroprotective potential

Chigurupati

Alharbi

Sharma

et al. 2021

Saudi Journal of Biological Sciences

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The current study primarily focused on the pharmacognostical and phytochemical screening of Canna indica and further analyzing the leaves extract for toxicological profile and neuroprotective potential. The microscopic, dry powder properties of the leaf material and phytochemical, physicochemical analysis was evaluated for pharmacognostical assessment. Dry leaves of C. indica were extracted using methanol and then further studied for both in vitro and in vivo toxicological study. The acute toxicity was measured by estimating the antioxidant defense system and anatomical impairment in the rat's organs. Also, the neuroprotective activity of the plant extract was assessed using anticholinesterase enzymatic inhibitory assay. The extract was found to be hemocompatible and showed absences of induction of behavioural changes. Likewise, no changes were seen on the anatomical structure of the rat’s organs. The methanolic extract portrayed a significant upsurge in the reduced glutathione level and showed a comparable acetylcholinesterase inhibition in a dosedependent manner with an IC50 value of 14.53 μg/mL compared to the standard Donepezil with an IC50 value of 13.31 μg/mL. C. indica has compelling pharmacognostical characteristics, good safety reports, and significant antioxidant as well as the neuroprotective potential that shows great potential for its further in-depth research for pharmacological use.

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“…The obtained extract was utilised for preliminary phytochemical investigation to find the presence of phytochemicals like the amino acids, flavonoids, anthocyanins, glycosides, phenols, proteins, saponins, carbohydrates, mucilage, alkaloids, gums, tannins and steroids by means of biochemical procedures. 14…”

Section: Preliminary Phytochemical Investigationmentioning

confidence: 99%

Bacterial Endophyte Inhabiting Durio zibethinus and its Radical Scavenging and Antidiabetic Potential

Chigurupati¹,

Vijayabalan²,

Palanimuthu³

et al. 2021

IJPER

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Background: Endophytes, markedly receiving interest and has been detected to be remarkable sources of bioactive metabolites. Endophytes, which are also called as endosymbionts have pulled into consideration in the discovery for novel bioactive compounds that can be utilized as new medications. In the current study, endophyte has been isolated from Durio zibethinus leaves. 16s rRNA partial genome sequencing was used to identify selected endophyte and the obtained bacterial endophytic crude extract (DZLM) was subjected for the antioxidant and antidiabetic investigations. Results: Cronobacter sakazakii was identifies as the endophye. Qualitative phytochemical test on DZLM confirmed the presence of phenols, carbohydrates, alkaloids, flavonoids, steroids, mucilage, and glycosides. The antioxidant assay of DZLM showed the good antioxidant potency with IC 50 ±SEM: 161±0.08 µg/mL for DPPH assay and IC 50 ±SEM: 126± 0.09 µg/mL for ABTS assay. Antidiabetic assay results demonstrated a dosedependent percentage inhibition of the enzymes. The results revealed good inhibition of α-amylase with IC 50 ±SEM: 239±0.08µg/mL and α-glucosidase inhibition with IC 50 ±SEM: 241±0.08 µg/mL for DZLM compared to the standard drug, acarbose. Conclusion: The study uncovered the fact that DZLM has a wellspring of natural source of antioxidant and antidiabetic agents and further studies are required for fractionation of the active constituents existing in DZLM and also their structural elucidation which supports the application of endo-symbionts as the basis of future natural medicines.

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Quantitative Estimation and Antimicrobial Potential of Ethanol Extract of Durio Zibethinus Murr. Leaves

Cited by 12 publications

References 9 publications

Screening Antimicrobial Potential for Malaysian Originated Tamarindus Indica Ethanolic Leaves Extract

Screening Antimicrobial Potential for Malaysian Originated Tamarindus Indica Ethanolic Leaves Extract

Pharmacological and pharmacognostical valuation of Canna indica leaves extract by quantifying safety profile and neuroprotective potential

Bacterial Endophyte Inhabiting Durio zibethinus and its Radical Scavenging and Antidiabetic Potential

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