Quantitative Estimation of Phytoplankton Species in Freshwater by Two Step Linear Regression Analysis Using Spectral Absorption Method

Lokuhewage, Asha U. M.; Naiki, Yasuhiro; Toyooka, Satoru

doi:10.1007/s10043-005-0420-4

Cited by 4 publications

(9 citation statements)

References 11 publications

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“…The aim of this work was to apply TSLR method (Lokuhewage et al 2005) for detection of two blue-green species from other algal species in freshwater. These two cyanobacterial species were different in their spectral characteristics (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

“…With the priori knowledge of unialgal species and YS together spectral absorption characteristics on concentration, we can distinguish contained unialgal species and YS only from spectral absorption curves (Lokuhewage et al 2005).…”

Section: Two Step Linear Regression Analysismentioning

confidence: 99%

“…To determine the dependence of spectral characteristics on the concentration of each algal groups and YS, we prepared pure samples at four different concentrations similar to Lokuhewage et al (2005). Four different concentrations (20, 100, 200, and 400 mg/l) of blue green 1 and 2, (50, 100, 200, and 400 mg/l) of green (20, 40, 80, and 150 mg/l) of brown-1 and 2 (150, 300, 600, and 900 mg/l) of YS were used for laboratory experiment.…”

Section: Field and Laboratory Experimentsmentioning

confidence: 99%

“…The resulting absorption spectra were normalized to the mean absorption between 360-740 nm wavelengths. Spectral similarities and differences can be assessed by computing the SI between the normalized spectra comprising TSLR for the five algal species and YS in the ndimensional space (39-D) similar to Lokuhewage et al (2005). Absorption spectra of algal species were compared using SI described previously by Millie et al 1997.…”

Section: Two Step Linear Regression Analysismentioning

confidence: 99%

“…To overcome this problem, passive method is preferable to discriminate problematic species of cyanobacteria. Recently proposed spectral absorption method can facilitate the discrimination of blue-green (Cyanophyceae) and eukaryotic algae (Chlorophyceae, Bacillariophyceae, and Dinophyceae) and of dissolved organic carbon (DOC) in fresh water samples, based on two step linear regression (TSLR) (Lokuhewage et al 2005). The specific spectral characteristics of each algal species may lead to differentiate them by TSLR method.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Two cyanobacterial strains can be distinguished from each other and other eukaryotic algae by spectral absorption method

Lokuhewage

Fujino

2011

Water Science and Technology

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Spectral absorption method based on two step linear regression analyses (TSLR) was applied for detection of two strains of cyanobacterium, Microcystis (blue-green algae) from eukaryotic algae. Both blue-green algae, algae and dissolved organic carbon (DOC) were considered from freshwater bodies in Kanto region, Japan. The results show that blue-green species can be detected from other algal species using absorption spectra of water samples. In this study statistical analysis was done by TSLR method, which determined the gradient vectors of single algal species and DOC. We believe that this method might be useful in environmental monitoring of freshwater algae.

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Section: Discussionmentioning

confidence: 99%

Section: Two Step Linear Regression Analysismentioning

confidence: 99%

Section: Field and Laboratory Experimentsmentioning

confidence: 99%

Section: Two Step Linear Regression Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Two cyanobacterial strains can be distinguished from each other and other eukaryotic algae by spectral absorption method

Lokuhewage

Fujino

2011

Water Science and Technology

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Bio-optical characterization of selected cyanobacteria strains present in marine and freshwater ecosystems

Wojtasiewicz

Stoń-Egiert

2016

J Appl Phycol

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The optical properties, i.e., absorption and scattering spectra of ten strains of cyanobacteria from the Baltic Sea and Pomeranian lakes (Aphanizomenon flos-aquae KAC 15, Microcystis aeruginosa CCNP 1101, Anabaena sp. CCNP 1406, Synechocystis salina CCNP 1104, Phormidium sp. CCNP 1317, Nodularia spumigena CCNP 1401, Synechococcus sp. CCNP 1108, Nostoc sp. CCNP 1411, Cyanobacterium sp. CCNP 1105, Pseudanabaena cf. galeata CCNP 1312) grown under low light conditions were investigated. Moreover, the chlorophylls, carotenoids, and phycobilin composition as well as the size structure of chosen cyanobacteria were measured. Studied species revealed high diversity both in optical properties with the absorption spectra similarity index ranging from 0.67 to 0.94 and the pigment composition. The chlorophyll-specific absorption coefficient at 440 nm aph*(440) varied between 0.017 and 0.065 m2 mg−1. The influence of the package effect was only observed in the case of large filamentous cyanobacteria like N. spumigena or Nostoc sp. Interestingly, the package effect factor Qa*(675) for large-celled Anabaena sp. was 0.92. Besides chlorophyll a, only echinenone, β-carotene, and phycocyanin were present in all analyzed cyanobacteria strains. Zeaxanthin, which is widely used as a marker pigment for cyanobacteria, was absent in the toxic N. spumigena and Anabaena sp., which are the species that occur in the Baltic Sea most frequently causing summer cyanobacterial blooms. The investigation also showed that the sample preservation technique can introduce some major errors within the absorption band affected by the phycocyanin absorption.

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Prediction of soil organic carbon with different parent materials development using visible-near infrared spectroscopy

Liu

Han

Zhang

et al. 2018

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Quantitative Estimation of Phytoplankton Species in Freshwater by Two Step Linear Regression Analysis Using Spectral Absorption Method

Cited by 4 publications

References 11 publications

Two cyanobacterial strains can be distinguished from each other and other eukaryotic algae by spectral absorption method

Two cyanobacterial strains can be distinguished from each other and other eukaryotic algae by spectral absorption method

Bio-optical characterization of selected cyanobacteria strains present in marine and freshwater ecosystems

Prediction of soil organic carbon with different parent materials development using visible-near infrared spectroscopy

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