Quantitative estimations of fusiforms in saliva from normal individuals and cases of acute ulcerative gingivitis

Hadi, Ammar; Russell, C.

doi:10.1016/0003-9969(68)90160-x

Cited by 9 publications

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References 8 publications

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“…The mouth consists of several micro-environments with different microbial flora; the gingival crevice and dental plaque are the principal sites of colonisation by gram-negative anaerobic bacilli (Socransky and Manganiello, 1971;Hardie and Bowden, 1974). The surface of the tongue is almost devoid of Bacteroides, and saliva contains a variable number of fusobacteria derived from the gingival crevice (Hadi and Russell, 1968;1969). Gibbons et al (1963Gibbons et al ( , 1964 and Loesche, Hockett and Syed (1972) found that 16.1% of the cultivable flora of the human gingival crevice, 4% of the flora from dental plaque in normal subjects, and 17% of the plaque flora in institutionalised subjects, were gram-negative anaerobic rods; Loesche and Gibbons (1965) devised a scheme for their identification.…”

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The Isolation and Identification of Bacteroides Spp. From the Normal Human Gingival Flora

Duerden

1980

Journal of Medical Microbiology

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(1972) was followed in all essential aspects; incubation was at 37°C in an atmosphere of H2 90% and COZ 10%. A slope of Simmons citrate medium seeded with Pseudomonas aeruginosa was included in each jar as a control.Specimens. Sterile toothpicks were used to collect samples of subgingival plaque from 20 normal healthy adults. The subjects were medical students aged 19-20 years; none had any gross oral or dental pathology and none was receiving antibiotic therapy.Isolation of Bacteroides. The methods and media were derived from those of Baird-Parker (1957), Loesche, Hockett and Syed (1971), Syed and Loesche (1973) and Williams et al. (1975); they were evaluated by Holbrook (1976) and Holbrook et al. (1978). The samples of plaque were seeded directly on to a sector of a plate of pre-reduced BM agar (see Williams et al., 1975) with kanamycin 75 pg/ml and vancomycin 2.5 pg/ml and the inoculum was streaked over the remainder of the plate. The plates were examined after anaerobic incubation for 48 h; all colony types were noted and representative colonies were subcultured from each specimen on to plain BM agar. After incubation for a further 48 h, any additional colony types were noted and further representative colonies were subcultured to a total of 10 from each specimen. The colony types were selected in approximate proportions to their comparative numbers on the primary isolation plate. The isolates were suspended in a freezing medium of Nutrient Broth No. 2 (Oxoid) with inactivated horse serum (Wellcome) 10% and glucose 1% and held at -70°C in a liquid-nitrogen container.IdentiJcation of isolates. The isolates were identified by the methods of Duerden et af. (1976, 1980). The tests were: colony morphology after 48-h incubation on blood agar; cell morphology in gram-stained smears from 48-h cultures on blood agar and in BM broth with cooked-meat particles (see Deacon, Duerden and Holbrook, 1978); pigment production on BM agar; haemolysis on human blood agar; motility in BM broth; antibiotic-disk resistance tests with neomycin 1000 pg, kanamycin 1000 pg, penicillin 2 units and rifampicin 15 pg disks; tolerance tests with taurocholate, deoxycholate, Victoria blue 4R and ethyl violet; biochemical tests for the production of indole, digestion of gelatin and hydrolysis of aesculin; fermentation tests with glucose, lactose, sucrose, rhamnose, trehalose, mannitol and xylose.

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The Isolation and Identification of Bacteroides Spp. From the Normal Human Gingival Flora

Duerden

1980

Journal of Medical Microbiology

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“…The bulk of the salivary organisms are streptococci, veillonellae, neisseriae and haemophili but all the following genera may also be represented: Staphylococcus, Micrococcus, Lactobacillus, Corynebacterium, Actinomyces, Rothia, Clostridium, Propionibacterium, Escherichia, Proteus, Pseudomonas, Klebsiella, Pasteurella, (Rosebury 1962;Richardson & Jones 1958;Hadi & Russell 1968;Bibby 1938). Some of these organisms are not common in sites other than the mouth.…”

Section: The Salivary Floramentioning

confidence: 99%

Bacteria in the Human Mouth

Russell

Melville

1978

Journal of Applied Bacteriology

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“…The bacterium is highly saccharolytic, and has been found to contribute up to 2.3% of the total viable counts in dental plaque (6). In saliva from patients with acute ulcerative gingivitis L. buccalis has been found in increased viable counts (7).…”

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Oligosaccharides obtained by partial hydrolysis of lipopolysaccharides from Leptotrichia buccalis

Birkeland

Hofstad

1985

European J Oral Sciences

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– Lipopolysaccharides (LPS) from Leptotrichia buccalis, strains LI 1, ATCG 14201 and ATCG 19616 were partially degraded with 1 % acetic acid. The acid soluble polysaccharides were subjected to gel filtration. These experiments showed that the carbohydrate moiety of LPS from ATGC 14201 and ATCG 19616 were similar, consisting ofa core oligosaccharide unsubstituted by 0‐side chains containing galactose, glucose and D‐glycero‐D‐manno‐heptose as neutral sugars. The core analogous fraction isolated from LI 1 LPS contained the same three neutral sugars, but in different ratios.

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Quantitative estimations of fusiforms in saliva from normal individuals and cases of acute ulcerative gingivitis

Cited by 9 publications

References 8 publications

The Isolation and Identification of Bacteroides Spp. From the Normal Human Gingival Flora

The Isolation and Identification of Bacteroides Spp. From the Normal Human Gingival Flora

Bacteria in the Human Mouth

Oligosaccharides obtained by partial hydrolysis of lipopolysaccharides from Leptotrichia buccalis

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