Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

Huang, Yuting; Wang, Liyang; Zhang, Xue; Su, Nan; Li, Heping; Oda, Yoshimitsu; Xing, Xin‐Hui

doi:10.1016/j.cjche.2021.01.009

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…46,47 It has been shown that RONS increases DNA damage and mutation rates in living cells at a much higher rate than conventional mutagenesis. 48 Typically, the concentration and type of reactive oxygen and reactive nitrogen (RONS) species in gas-and liquid-phase plasmas are measured by UV absorption. 47,49,50 F I G U R E 3 Schematic representation of the types of plasma active substances, diagnostic tools, and mutagenic mechanisms.…”

Section: Asmentioning

confidence: 99%

“…For example, it causes secondary excitation to produce other active species, DNA breakage, and so forth. 48,63,64 The recent emergence of molecular beam MS is emerging as an effective diagnostic strategy for plasma-active species due to the use of graded pumping sampling that allows active species to be flown directly into the mass spectrometer for analysis under almost collision-free conditions. 65 It has been used to measure the absolute density of neutral substances such as NO, atomic oxygen, and O 3 .…”

Section: Msmentioning

confidence: 99%

“…The energy distribution is highly relevant for the subsequent production of active species and their biological effects. For example, it causes secondary excitation to produce other active species, DNA breakage, and so forth 48,63,64 …”

Section: Artpmentioning

confidence: 99%

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Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

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At present, atmospheric and room‐temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment‐friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.

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Section: Asmentioning

confidence: 99%

Section: Msmentioning

confidence: 99%

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Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Li,

Shen,

Ding

et al. 2024

Cell Biochemistry & Function

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“…Mutagenesis methods as effective approaches have been applied in exploring the unknown molecular functions of microbes by combining modern omics analysis ( Huang et al, 2021 ). DNA sequencing is a gold standard for determining the exact nature of a mutation and has been widely used for DNA diagnostics and functional studies of genes of interes.…”

Section: Breeding Techniquesmentioning

confidence: 99%

Edible and medicinal fungi breeding techniques, a review: Current status and future prospects

Dong

Miao

Feng

et al. 2022

Current Research in Food Science

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“…Microbial mutation breeding is an important technique, which has been broadly employed for microbial improvement, especially in the fields of biotechnology, biomanufacturing, food fermentation, and environmental protection ( Brock, 1976 ). Mutagenesis tools, including chemical mutagens, physical or biological approaches, are used to increase mutation rate and accelerate the subsequent evolutionary process to obtain mutant strains with desirable phenotypes ( Huang et al, 2021 ). Among them, Atmospheric Room Temperature Plasma (ARTP) mutagenesis technology caused more serious DNA damage and a higher mutation rate among all the mutagenesis methods ( Zhang et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Mutation breeding ofAspergillus nigerby atmospheric room temperature plasma to enhance phosphorus solubilization ability

Peng

Xiao

Zhang

et al. 2022

PeerJ

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Background Phosphorus (P) is abundant in soils, including organic and inorganic forms. Nevertheless, most of P compounds cannot be absorbed and used by plants. Aspergillus niger v. Tiegh is a strain that can efficiently degrade P compounds in soils. Methods In this study, A. niger xj strain was mutated using Atmospheric Room Temperature Plasma (ARTP) technology and the strains were screened by Mo-Sb Colorimetry with strong P-solubilizing abilities. Results Compared with the A. niger xj strain, setting the treatment time of mutagenesis to 120 s, four positive mutant strains marked as xj 90–32, xj120–12, xj120–31, and xj180–22 had higher P-solubilizing rates by 50.3%, 57.5%, 55.9%, and 61.4%, respectively. Among them, the xj120–12 is a highly efficient P solubilizing and growth-promoting strain with good application prospects. The growth characteristics such as plant height, root length, and dry and fresh biomass of peanut (Arachis hypogaea L.) increased by 33.5%, 43.8%, 43.4%, and 33.6%, respectively. Besides available P, the chlorophyll and soluble protein contents also vary degrees of increase in the P-solubilizing mutant strains. Conclusions The results showed that the ARTP mutagenesis technology can improve the P solubilization abilities of the A. niger mutant strains and make the biomass of peanut plants was enhanced of mutant strains.

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Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

Cited by 11 publications

References 33 publications

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

Edible and medicinal fungi breeding techniques, a review: Current status and future prospects

Mutation breeding ofAspergillus nigerby atmospheric room temperature plasma to enhance phosphorus solubilization ability

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