Quantitative Evaluation of Melanoma Cell Invasion in Three-Dimensional Confrontation Cultures In Vitro Using Automated Image Analysis

Smolle, Josef; Helige, Christine; Soyer, H. P.; Hoedl, Stefan; Popper, Helmut; Stettner, Haro; Kerl, Helmut; Tritthart, H. A.; Kresbach, H

doi:10.1111/1523-1747.ep12873976

Cited by 20 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The value of Invaslog has been specially proven to be a strong quantitative measure for the invasiviness in the cancer/host spheroid model, 8,9 and is therefore the reference value for the fractal analysis.…”

Section: Confocal Microscope Andmentioning

confidence: 99%

See 1 more Smart Citation

Fractal Dimension for a Cancer Invasion Model

2001

View full text Add to dashboard Cite

A long-established investigation method for in vitro cancer invasion using cancer cell spheroid and normal cell spheroid confrontation for the quantitative determination of invasion was expanded to make use of fractal methods. The box-counting method, the sausage method and a local method were used in comparison with image pre-processing steps such as median filtering, closing and opening, or hole filling and scrapping, and artificially added noise images. It was possible to examine the potential role of the fractal capacity dimension for this invasion model. Several aspects such as the absolute value and variance of the different methods were taken into account and compared to well established quantitative parameters. The fractal approach led to very promising results which improved the determination and examination of the invasion process of cancer.

show abstract

Section: Confocal Microscope Andmentioning

confidence: 99%

“…Outgoing from the well-established and objective invasion parameter Invaslog 8,9 and the implementation of a confocal laser scanning method, 10,11 we expanded the above model by the addition of fractal analysis of long-term measurements of the invasion process.…”

Section: Introductionmentioning

confidence: 99%

Fractal Dimension for a Cancer Invasion Model

2001

View full text Add to dashboard Cite

show abstract

“…Instead of aggregates, biopsy specimens , and serum-free confrontations have been shown to be feasible with certain types of cells 25 . Generally, the interaction is described in accordance with a semi quantitative scale 26 however, computer-assisted automated image analysis systems have also been developed 27,28 . The latter aim to provide quantitative information on the extent of tumor cell invasion.…”

Section: Discussionmentioning

confidence: 99%

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Bracke¹,

Roman

Stevens

et al. 2015

JoVE

View full text Add to dashboard Cite

The goal of the chick heart assay is to offer a relevant organ culture method to study tumor invasion in three dimensions. The assay can distinguish between invasive and non-invasive cells, and enables study of the effects of test compounds on tumor invasion. Cancer cells -either as aggregates or single cells -are confronted with fragments of embryonic chick heart. After organ culture in suspension for a few days or weeks the confronting cultures are fixed and embedded in paraffin for histological analysis. The three-dimensional interaction between the cancer cells and the normal tissue is then reconstructed from serial sections stained with hematoxylin-eosin or after immunohistochemical staining for epitopes in the heart tissue or the confronting cancer cells. The assay is consistent with the recent concept that cancer invasion is the result of molecular interactions between the cancer cells and their neighbouring stromal host elements (myofibroblasts, endothelial cells, extracellular matrix components, etc.). Here, this stromal environment is offered to the cancer cells as a living tissue fragment. Supporting aspects to the relevance of the assay are multiple. Invasion in the assay is in accordance with the criteria of cancer invasion: progressive occupation and replacement in time and space of the host tissue, and invasiveness and non-invasiveness in vivo of the confronting cells generally correlates with the outcome of the assay. Furthermore, the invasion pattern of cells in vivo, as defined by pathologists, is reflected in the histological images in the assay. Quantitative structure-activity relation (QSAR) analysis of the results obtained with numerous potentially anti-invasive organic congener compounds allowed the study of structure-activity relations for flavonoids and chalcones, and known anti-metastatic drugs used in the clinic (e.g., microtubule inhibitors) inhibit invasion in the assay as well. However, the assay does not take into account immunological contributions to cancer invasion. Video LinkThe video component of this article can be found at

show abstract

“…From experimental studies, il is evident that active motility is a crucial feature of metastatic tumor cells (1,2,16,17). For the formation of clinically relevant metastases, however, molility must be ac-companied by the ability for proliferation.…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of proliferation and motility in primary malignant melanoma of the skin

1992

Self Cite

View full text Add to dashboard Cite

The metastatic cascade depends on the presence of tumor cells which are capable of proliferation as well as of invasion with active motility. In the present study, it was examined whether the demonstration of both features in the primary lesion of malignant melanoma of the skin carries prognostic significance. Proliferation was assessed by mitotic counts and Ki 67 staining, and motility was estimated by image analysis and comparison of the image analysis results with computer simulations; 27 cases of primary malignant melanoma with a maximum vertical tumor thickness exceeding 1 mm were prospectively sampled. Mitotic counts were carried out on H & E stained sections, image analysis of the tumor pattern on S-100 immunostained paraffin slides, and Ki 67 labeling of actively cycling cells was evaluated on frozen sections. Neither proliferation nor pattern analysis alone provided a significant prognostic result with respect to overall survival and to metastasis free survival. The estimates of motility, derived from a combination of pattern analysis and proliferation values, however, proved to be significant predictors of overall survival and metastasis-free survival (log rank test: p less than or equal to 0.05). The motility features were superior to Clark level and Breslow index in this set of cases. The results demonstrate that the assessment of tumor cell proliferation and motility in histological sections may reflect the metastatic potential of primary malignant melanomas of the skin.

show abstract

Quantitative Evaluation of Melanoma Cell Invasion in Three-Dimensional Confrontation Cultures In Vitro Using Automated Image Analysis

Cited by 20 publications

References 11 publications

Fractal Dimension for a Cancer Invasion Model

Fractal Dimension for a Cancer Invasion Model

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Prognostic significance of proliferation and motility in primary malignant melanoma of the skin

Contact Info

Product

Resources

About