Quantitative evaluation of skin disorders in type 1 diabetic mice by in vivo optical imaging

Feng, Wei; Zhang, Chao; Yu, Tingting; Zhu, Dan

doi:10.1364/boe.10.002996

Cited by 11 publications

(8 citation statements)

References 49 publications

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“…A recent study have verified that UVB irradiation caused the skin dermis became thinner after H&E staining ( Blackstone et al, 2020 ), our study found the same result. In our study, the number of collagen fibers in the skin of mice with UVB-induced skin damage decreased, and at the same time, collagen fibers appeared atrophy, breakage and stickiness ( Feng et al, 2019 ). Otherwise, the number of mast cells in the dermis layer increases significantly after UVB irradiation, which indicate the aggravation of skin inflammation ( Kim et al, 2014a ).…”

Section: Discussionmentioning

confidence: 55%

Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway

Zhou

et al. 2021

Front. Pharmacol.

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Long-term exposure to UVB (280–320 nm) can cause oxidative skin damage, inflammatory injury, and skin cancer. Research on nicotinamide mononucleotide (NMN) and lactic acid bacteria (LAB) with regard to antioxidation, anti-inflammation, and prevention of other age-related diseases has received increasing attention. In the present study, the in vitro antioxidant analysis showed that NMN combined with Lactobacillus fermentum TKSN041 (L. fermentum TKSN041) has a high scavenging ability on hydroxyl (OH), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and it also possess a good total antioxidant capacity. The animal experimental results show that NMN combined with LAB maintained normal liver morphology of mice and reduced pathological damage to murine skin. NMN combined with LAB significantly increased the serum levels of total superoxide dismutase (T-SOD), catalase (CAT), and interleukin (IL)-10, but reduced the levels of malondialdehyde, advanced glycation end products, tumor necrosis factor (TNF)-α, and IL-6. NMN combined with LAB increased T-SOD, CAT, IL-10, Na+-K+-ATPase, and NAD+ levels in the skin, but reduced TNF-α level in the skin. NMN combined with LAB increased the mRNA expression levels of SOD1, CAT, glutathione (GSH), inhibitor of NF-κB (IκB-α), IL-10, AMP-activated protein kinase (AMPK), adaptor protein, phosphotyros ineinteraction, PH domain and leucine zipper containing 1 (APPL1), peroxisome proliferator-activated receptor γ co-activator-1α (PGC-1α), and forkhead transcription factor O (FOXO) in the skin and liver, but decreased the mRNA expression levels of nuclear factor (NF)-κBp65, TNF-α, IL-6, and rapamycin target protein (mTOR). NMN combined with LAB increased the protein expression levels of AMPK, IκB-α, SOD1, and CAT in the skin tissues and reduced protein expression of NF-κBp65. NMN combined with L. fermentum TKSN041 improved murine skin damage caused by UVB irradiation, and the protective mechanism may be related to activation of the AMPK signaling pathway. The results of this study are expected to provide a reference for preventing and the treating skin photoaging.

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Section: Discussionmentioning

confidence: 55%

Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway

Zhou

et al. 2021

Front. Pharmacol.

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“…In vivo, OTC has been applied to the skin for visualizing blood flow in the skin (Vargas et al, 2003;Wang et al, 2013aWang et al, , 2013bZhu et al, 2010) and cutaneous vascular permeability in mice suffering from diabetes (Feng et al, 2019) and for improving the imaging of diseased skin in a variety of skin diseases such as hemangioma and epidermoid cyst (Shan et al, 2012) as well as increasing laser penetration into the dermis during tattoo removal (Liu et al, 2015).…”

Section: Applications Of Optical Clearing and Volumetric Imagingmentioning

confidence: 99%

“…With this method, it is possible to generate a 3D image without physically sectioning the tissue. OTC can be used in ex vivo 3D anatomical investigations to study skin pathology, for instance, epidermal hyperplasia in psoriatic skin (Abadie et al, 2018) or neuronal innervation in pruritic skin (Tan et al, 2019) as well as in in vivo skin imaging to measure dermal blood flow (Wang et al, 2013b) and alterations in skin structure in mice with diabetes (Feng et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Research Techniques Made Simple: Optical Clearing and Three-Dimensional Volumetric Imaging of Skin Biopsies

Tan

Chiam

Zhang

et al. 2020

Journal of Investigative Dermatology

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Skin histology is traditionally carried out using two-dimensional tissue sections, which allows for rapid staining, but these sections cannot accurately represent three-dimensional structures in skin such as nerves, vasculature, hair follicles, and sebaceous glands. Although it may be ideal to image skin in a three-dimensional manner, it is technically challenging to image deep into tissue because of light scattering from collagen fibrils in the dermis and refractive index mismatch owing to the presence of differing biological materials such as cytoplasm, and lipids in the skin. Different optical clearing methods have been developed recently, making it possible to render tissues transparent using different approaches. Here, we discuss the steps involved in tissue preparation for three-dimensional volumetric imaging and provide a brief overview of the different optical clearing methods as well as different imaging modalities for three-dimensional imaging.

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“…Tissue optical clearing (TOC) is a convenient technique to increase the penetration depth of thick tissues [17][18][19][20][21][22]. The replacement of free water with optical clearing agents of higher refractive index, such as glycerin, causes refractive index matching between the scatterers and their surrounding media to reduce the scattering coefficient of biological tissues, which is often considered as one of the major mechanisms of TOC [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Probing Dynamic Variation of Layered Microstructure Using Backscattering Polarization Imaging

Shao

Zhu

et al. 2022

Photonics

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Polarization imaging can quantitatively probe the microscopic structure of biological tissues which can be complex and consist of layered structures. In this paper, we established a fast-backscattering Mueller matrix imaging system to characterize the dynamic variation in the microstructure of single-layer and double-layer tissues as glycerin solution penetrated into the samples. The characteristic response of Mueller matrix elements, as well as polarization parameters with clearer physics meanings, show that polarization imaging can capture the dynamic variation in the layered microstructure. The experimental results are confirmed by Monte Carlo simulations. Further examination on the accuracy of Mueller matrix measurements also shows that much faster speed has to be considered when backscattering Mueller matrix imaging is applied to living samples.

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Quantitative evaluation of skin disorders in type 1 diabetic mice by in vivo optical imaging

Cited by 11 publications

References 49 publications

Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway

Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway

Research Techniques Made Simple: Optical Clearing and Three-Dimensional Volumetric Imaging of Skin Biopsies

Probing Dynamic Variation of Layered Microstructure Using Backscattering Polarization Imaging

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