Quantitative evaluation of tissue invasion by wild type, hyphal and SAP mutants of Candida albicans, and non‐albicans Candida species in reconstituted human oral epithelium

Abstract

RHOE model in combination with computerized image analysis permits for the first time, the assessment of invasive potential of Candida species in a quantitative manner. The differential tissue invasive patterns of various C. albicans isolates, their mutants and other Candida species are also described.

Candida albicans Biofilms Produce More Secreted Aspartyl Protease than the Planktonic Cells

“…Among the virulence attributes, the secreted aspartyl proteases (Saps) seem to have a prominent role, due to their capacity in to damage the integrity of structural proteins and to affect the tissue architectural patterns. 2) Despite their importance, to our knowledge, all published studies thus far have focused on Sap production in yeasts either grown on semi-solid agar or liquid culture media and ours is the first comparative study of this phenomenon in both liquid and solid substrata. Here, we evaluate the differences in secretion of Candida Saps using a simple, low-cost methodology.…”

Pathogenic Attributes of Non-Candida albicans Candida Species Revealed by SEM

“…Furthermore, different intra-species adherence ability has been reported for Candida species (reviewed in Silva et al, 2011). For instance, C. tropicalis exhibited higher ability to colonize reconstituted human epithelium (RHE) than did C. parapsilosis and Candida glabrata (Jayatilake et al, 2006). Adherence of C. albicans to epithelial cells is greater than that of C. parapsilosis (Lima-Neto et al, 2011).…”

“…According to the literature, the invasiveness of non-albicans Candida species is variable among species. For instance, in RHE model C. tropicalis exhibited higher ability to invade this tissue than did C. parapsilosis and C. glabrata (Jayatilake et al, 2006).…”

“…For C. albicans, strains lacking hyphal formation exhibited lower ability to invade tissue compared with wild-type strains (Jayatilake et al, 2006).…”

Differential Interaction of the Two Related Fungal SpeciesCandida albicansandCandida dubliniensiswith Human Neutrophils

“…Nevertheless, it has been shown that even though both Candida species release a chemotactic factor for neutrophils, the chemotaxis toward C. dubliniensis is enhanced compared with C. albicans (18). Studies using epithelial cell infection models demonstrated that C. dubliniensis causes minimal invasion (5,19) and cytokine production (20,21) compared with C. albicans, and that this effect is attributed to the ability of the latter to switch from yeast to hyphal morphology under cell culture conditions (21).…”