Quantitative Examination of the Simple Micelle–Mixed Micelle Coexistence for the Taurocholate–Lecithin System by GP-HPLC

Estimation of the Taurocholate to Lecithin Molar Ratio of Mixed Micelles in Taurocholate‐Lecithin Solutions by an Interaction Model and Laser Light‐Scattering Measurements

“…Interestingly, the K values for L concentrations of 6 mM (K = 4.33), 12 mM (K = 2.06), and 21 mM (K = 1.91) were very close to those obtained in previous dialysis [9][10][11] and GP-HPLC studies. 24 (K = 2.06) and 21 mM (K = 1.91) were close to the value (K = 2) previously assumed for the interaction study. 13 This consistency confirms the reliability of K values determined in dialysis studies.…”

“…Although a technique of gel-permeation, high-performance liquid chromatography (GP-HPLC), which supports dialysis data, has been recently employed in studies of the coexistence of simple TC micelles and mixed TC-L micelles, 24 the resulting K values were~20% lower than those obtained from equilibrium dialysis studies. [9][10][11] Moreover, the total aggregation number (~10) in simple TC micelles estimated by GP-HPLC was out of the range (5 ± 2) reported in the literature.…”

Sustained release micellar carrier systems for iontophoretic transport of dexamethasone across human sclera

“…The passive permeability coefficient of DEX in the control (2.3 × 10 −5 cm/s) was approximately 3 times higher than that of SMM (5.6 × 10 −6 cm/s) and UMM (5.8 × 10 −6 cm/s). The passive permeability coefficient in the control experiment was higher than those of SMM and UMM because DEX loaded in the mixed micelles could not permeate through the MWCO 1000 dialysis membrane [34]. As only free DEX can diffuse across the membrane into the receptor, this allows the determination of the encapsulation efficiencies of the carrier systems using Eq.…”

“…The concentration of TA in the SSM solution was 28 mg/mL in all experiments unless otherwise stated. This concentration was selected because it was the same TA concentration as of the simple micelles co-existing in the 95 mg/mL mixed micelle system according to calculations based on a previous study [34]. …”

Separation and Characterization of the Colloidal Phases Produced on Digestion of Common Formulation Lipids and Assessment of Their Impact on the Apparent Solubility of Selected Poorly Water-Soluble Drugs

“…Previous studies have measured the IMC of similar systems using ultrafiltration and equilibrium dialysis methods and have reported significantly higher IMCs than those described here 24,25. However, the IMC measured by these methods quantifies the total nonmixed micellar and nonvesicular BS concentration (the terminology NMBS (nonmixed micelle bile salt) was used in some of these studies26,27), and this IMC includes BS associated with simple BS micelles. In our experiments, only the concentration of free BS in equilibrium with (and external to) all the colloidal structures present (including simple micelles) has been measured, and is therefore substantially lower than the previously defined NMBS concentration.…”