Quantitative fast fractionation of a pool of polyclonal antibodies by immunoaffinity membrane chromatography

Platonova, G. A.; Панкова, Г. А.; Il’ina, Irma Ye.; Vlasov, G. P.; Tennikova, Tatiana

doi:10.1016/s0021-9673(99)00672-x

Cited by 60 publications

(19 citation statements)

References 42 publications

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“…[ 6 ] Due to this reactivity, epoxy based polymers have been used for a number of applications from the separation of biomolecules [ 7 ] to covalent enzyme immobilization. copolymer of ethyleneoxide (EO) and propyleneoxide (PO)) was required to produce a stable w/o HIPE, due to the relative hydrophilicity of GMA and ethyleneglycol dimethacrylate (EGDMA) crosslinker.…”

Section: Extending the Range Of Monomers Applicable To Emulsion Templmentioning

confidence: 99%

Functional Porous Polymers by Emulsion Templating: Recent Advances

2010

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Section: Extending the Range Of Monomers Applicable To Emulsion Templmentioning

confidence: 99%

Functional Porous Polymers by Emulsion Templating: Recent Advances

2010

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“…Due to the limited pressure stability of Cellufine 1 sulfate under operating conditions (<2 bar, recommendation of manufacturer) the flow rate is restricted resulting in suboptimal productivity. Membrane adsorbers, which may overcome such drawbacks, have been already used for affinity separation of different target molecules, for example, recombinant proteins (Cattoli et al, 2006;Mellado et al, 2007), antibodies (Boi et al, 2006b;Castilho et al, 2002;Platonova et al, 1999), lectins (Boi et al, 2006a;Sorci et al, 2006) or influenza virus particles (Opitz et al, 2007a). In addition, membrane-based ion-exchange chromatography was successfully applied for purification of several other virus species, including for example insect baculoviruses (Wu et al, 2007), mosquito-specific parvoviruses (Czermak et al, 2008;Specht et al, 2004), alphaherpesviruses (Karger et al, 1998), adenovirus vectors (Peixoto et al, 2008) or influenza viruses (Kalbfuß et al, 2007b).…”

Section: Introductionmentioning

confidence: 99%

Sulfated membrane adsorbers for economic pseudo‐affinity capture of influenza virus particles

Opitz

Lehmann

Reichl

et al. 2009

Biotech & Bioengineering

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Strategies to control outbreaks of influenza, a contagious respiratory tract disease, are focused mainly on prophylactic vaccinations in conjunction with antiviral medications. Currently, several mammalian cell culture-based influenza vaccine production processes are being established, such as the technologies introduced by Novartis Behring (Optaflu) or Baxter International Inc. (Celvapan). Downstream processing of influenza virus vaccines from cell culture supernatant can be performed by adsorbing virions onto sulfated column chromatography beads, such as Cellufine sulfate. This study focused on the development of a sulfated cellulose membrane (SCM) chromatography unit operation to capture cell culture-derived influenza viruses. The advantages of the novel method were demonstrated for the Madin Darby canine kidney (MDCK) cell-derived influenza virus A/Puerto Rico/8/34 (H1N1). Furthermore, the SCM-adsorbers were compared directly to column-based Cellufine sulfate and commercially available cation-exchange membrane adsorbers. Sulfated cellulose membrane adsorbers showed high viral product recoveries. In addition, the SCM-capture step resulted in a higher reduction of dsDNA compared to the tested cation-exchange membrane adsorbers. The productivity of the SCM-based unit operation could be significantly improved by a 30-fold increase in volumetric flow rate during adsorption compared to the bead-based capture method. The higher flow rate even further reduced the level of contaminating dsDNA by about twofold. The reproducibility and general applicability of the developed unit operation were demonstrated for two further MDCK cell-derived influenza virus strains: A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004. Overall, SCM-adsorbers represent a powerful and economically favorable alternative for influenza virus capture over conventional methods using Cellufine sulfate.

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“…In one case, synthetic peptides were immobilized in monolithic disks and used to isolate their corresponding antibodies from human serum [102]. The same study examined the use of multidimensional chromatography on monoliths for the isolation of a monoclonal antibody from ascites fluid by using tandem disks containing an anion-exchange material or immobilized protein A.…”

Section: Immunoaffinity Chromatography and Related Methodsmentioning

confidence: 99%

“…A related report described the use of GMA/ EDMA disks containing a peptide with the sequence EYKSWEYC for the purification of factor VIII from plasma [108]. Synthetic pentadecapeptides, hexadecapeptides, and bradykinin were immobilized on GMA/EDMA monoliths for the purification of antibodies against these agents [102]. The immobilization of such ligands on GMA/EDMA disks was optimized using a peptide with an affinity for chicken egg lysozyme as a model [109].…”

Section: Dye-ligand and Biomimetic Affinity Chromatographymentioning

confidence: 99%

Affinity monolith chromatography

Mallik

Hage²

2006

J of Separation Science

190

282

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The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.

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Quantitative fast fractionation of a pool of polyclonal antibodies by immunoaffinity membrane chromatography

Cited by 60 publications

References 42 publications

Functional Porous Polymers by Emulsion Templating: Recent Advances

Functional Porous Polymers by Emulsion Templating: Recent Advances

Sulfated membrane adsorbers for economic pseudo‐affinity capture of influenza virus particles

Affinity monolith chromatography

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