Quantitative Fluorescence Image Analysis in Bladder Cancer Screening

Hemstreet, George P.; Hurst, Robert E.; Bass, Rebecca A.; Rao, Jian Yu

doi:10.1097/00043764-199009000-00013

Cited by 19 publications

(7 citation statements)

References 18 publications

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“…PAP is sensitive to high-grade malignancies but is generally insensitive to low-grade tumors [Nelson, 1985]. Various studies suggest an overall sensitivity of 66% for voided urine PAP cytology across the range of grades and stages for transitional cell tumors [Hemstreet et al, 1986], and 3) Quantitative fluorescence image analysis (QFIA) [West, 1965;Bass et al, 1987;Farrow, 1990;Hemstreet et al, 1990] is an evolving technology for quantitating biomarkers in exfoliated cells. This methodology detects early precancerous changes by measuring the amount of DNA or other biomarkers present in exfoliated uroepithelial cells.…”

Section: Dhrs Screening Testsmentioning

confidence: 99%

The Drake Health Registry Study: Findings from fifteen years of continuous bladder cancer screening

Marsh

Cassidy

2003

American J Industrial Med

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Section: Dhrs Screening Testsmentioning

confidence: 99%

The Drake Health Registry Study: Findings from fifteen years of continuous bladder cancer screening

Marsh

Cassidy

2003

American J Industrial Med

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“…Because quantitative rather than qualitative differences in gene expression and protein levels probably are responsible for most of the differences between malignant and normal phenotypes (13), quantitative fluorescence image analysis was used to quantify a panel of phenotypic markers in single cells from the biopsy specimens. Because of its visual morphologic component, quantitative fluorescence image analysis can link conventional morphologic assessment with quantitative biochemical markers at the single-cell level (14)(15)(16). The markers included quantitative fluorescence image analysis cytology (14), a combination of visual morphologic classification and identifying cells with DNA in excess of 5C (2C = amount of signal equivalent to the diploid chromosome complement), which is a marker for genetic instability; the p300 tumor-related antigen detected by the M344 monoclonal antibody (17); the differentiation-related proteins epidermal growth factor receptor (EGFR) (18,19) and G-actin (the globular monomeric precursor protein to actin filaments); and p185, the product of the HER-2/neu oncogene.…”

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confidence: 99%

Alterations in phenotypic biochemical markers in bladder epithelium during tumorigenesis.

Rao

Hemstreet

Hurst

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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Phenotypic biochemical markers of oncogenesis and differentiation were mapped in bladder biopsies to investigate changes that occur in bladder tumorigenesis and to identify markers for increased bladder cancer risk. Touch preparations from biopsy specimens from 30 patients were obtained from tumors, the adjacent bladder epithelium, and random distant bladder epithelium. Markers, including DNA ploidy, epidermal growth factor receptor (EGFR), and oncoproteins, were quantified in individual cells by using quantitative fluorescence image analysis. Cluster analysis revealed the markers fell into three independent groups: (0) G-actin and EGFR; (it) ploidy, cytology, and p185 (HER-2/neu oncoprotein) (ERBB2); and (iil) p300, a low-grade tumor antigen. Each marker displayed a gradient of abnormality from distant field to adjacent field to tumor. Different patterns for each marker suggested a developmental sequence of bladder cancer oncogenesis; G-actin was altered in 58% of distant biopsies (vs. 0/6 normals, P < 0.001), ploidy and cytology were altered in <20% of distant fields and =80% of tumors, and the other markers were intermediate. Patterns of EGFR and p185 suggest lowand high-grade tracks diverge early (P < 0.05 by MannWhitney U test for EGFR and ANOVA for p185). In conclusion, this study shows that a sequence of phenotypic changes accompanies development and progression of bladder cancers. Biochemical alterations in cells of the bladder field are often detectable before abnormal pathology, and markers previously thought to be limited to tumors were found in the field. The hierarchy of expression may be useful in identifying high-risk patients, assessing completeness of response to therapy, and monitoring and predicting recurrence.Decreasing future deaths from bladder cancer will require a strategy of prevention, based in part upon identification of individuals at risk for invasive metastatic disease well before it becomes symptomatic. Recognition that clinical cancer is the end-point of the underlying disease of carcinogenesis (1) suggests that appropriate intervention with individuals determined to be at risk could prevent development of lifethreatening disease.The concept of "field cancerization" or "field disease," introduced in 1953 (2), suggests that the whole field of tissue is exposed to carcinogen and is at increased risk for developing cancers, even though individual lesions are of clonal origin. The field disease theory as applied to bladder cancer has provided a central organizing theory for understanding these multifocal, frequently recurrent tumors (3-5). The normal bladder, or any other solid organ, represents a complex ecosystem ofinteracting epithelial and stromal cells, and years are required for the progressive subversion of growth and differentiation controls (6-8) to result in eventual emergence of cells capable of at least partial autonomousThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" i...

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“…[7][8][9][10][11] In general, morphometry added objectivity to reports otherwise highly dependent on diagnostic experience. 3 Image cytometry on Feulgen-stained exfoliative material from the bladder introduced the biometric parameter ploidy.…”

mentioning

confidence: 99%

Quick‐staining urinary cytology and bladder wash image analysis with an integrated risk classification

Wiener

Remkes

Schatzl

et al. 1999

Cancer

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Quantitative Fluorescence Image Analysis in Bladder Cancer Screening

Cited by 19 publications

References 18 publications

The Drake Health Registry Study: Findings from fifteen years of continuous bladder cancer screening

The Drake Health Registry Study: Findings from fifteen years of continuous bladder cancer screening

Alterations in phenotypic biochemical markers in bladder epithelium during tumorigenesis.

Quick‐staining urinary cytology and bladder wash image analysis with an integrated risk classification

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