Quantitative Gas Chromatography of Amino Acids. Preparation of n-Butyl N-Trifluoroacetyl Esters.

Abstract

A procedure was developed for preparing n-butyl N-trifluoroacetyl esters of amino acids for use in analysis by gas liquid chromatography. This derivative was found to have advantages over others investigated. Single chromatographic peaks were obtained for all of the common protein amino acids except tryptophan and arginine. Tryptophan gave two peaks but could be converted into a single derivative by longer acylation. Reproducibility of response was found to be good for derivatives carried through the entire chemical and chromatographic procedure, and no problems were indicated in the analysis of mixtures. Under anhydrous conditions the derivatives were stable with respect to time, and yield of derivative was found to be above 96% for each of six representative amino acids.

Determination of amino acid production rate of a marine phytoplankton population with 13C and gas chromatography‐mass spectrometry1

“…Amino acids are converted to their N-trifluoroacetyl n-butyl esters (TAB esters) (Lamkin and Gehrke 1965;Kaiser et al 1974) and then analyzed by GC-MS. The accuracy of the estimate of the 13C atom% of each amino acid was investigated by analyzing standards with several different 13C ratios.…”

Studies on the Reaction Conditions of DOPA Production withAlcaligenes faecalis.

“…acids were prepared according to the method of Gehrke et al 9 ,IO) The N-trifluoroacetyl-n-butyl esters of amino acids were dissolved in anhydrous chloroform prior to gas chromatography, and analyzed with a gas chromatograph (Hitachi Ltd., Model K-S3) fitted with a hydrogen flame ionization detector using herium as the carrier gas. Glass column (1 SO x 0.3 cm i.d.)…”

Separation and determination of trinitrotoluene isomers by gas chromatography

“…The 2,3,4-and 2,4,5-TNT isomers were laboratory synthesized and purified by selective solvent extraction (5). Laboratory nitration of rn-nitrotoluene gave a product rich in unsymmetrical T N T and DNT isomers from which the 2,3,6-, 2,3,5-, and 3,4,5-TNT isomers and 2,3-DNT were separated and collected in pure form by gas Identities and purities of all the isomers were established from infrared (5) and N M R spectra. N M R spectra were obtained for each T N T isomer (except for 3,4,5-TNT) and will be the subject of a separate report.…”