Quantitative Histochemistry of the Nephron. Vi. Hydroxyproline in the Human Glomerulus*

Bonting, S.L.; Bruin, Hendrina de; Pollak, Victor E.

doi:10.1172/jci104242

Cited by 20 publications

(6 citation statements)

References 9 publications

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“…These observations indicate that some form of collagen is present in the basement membranes even though most observers have not found collagen fibrils in normal mammalian renal glomeruli (4--10). The high proportion of hydroxyproline present on amino acid analysis of renal glomerular basement membranes of the dog (11,12) and of man (13)(14)(15) also strongly suggests the presence of collagen.…”

mentioning

confidence: 99%

Demonstration by Immunofluorescence of the Fixation of Perfused Antibody to Human Collagen in Human Kidney

Rothbard

Watson

1967

The Journal of Experimental Medicine

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Immunofluorescence has demonstrated that antibody prepared in rabbits against acid-soluble collagen from rat, mouse, guinea pig, or chicken, injected into the circulation of the homologous species, becomes fixed in the basement membranes of the renal glomeruli and tubules (1, 2). In vitro and in vivo experiments have indicated that the antibody is directed specifically toward collagen and that it exhibits species specificity (1-3). These observations indicate that some form of collagen is present in the basement membranes even though most observers have not found collagen fibrils in normal mammalian renal glomeruli (4--10). The high proportion of hydroxyproline present on amino acid analysis of renal glomerular basement membranes of the dog (11, 12) and of man (13-15) also strongly suggests the presence of collagen.It therefore seemed desirable to extend our observations in animals to human renal glomeruli. The injection of collagen antibody into the circulation of the animals, a procedure not feasible in man, gave better defined and more intense fluorescence than the method in which frozen kidney sections were treated with the anticollagen serum (1). We therefore attempted to approximate the injection method by perfusing the collagen antibody through the renal artery of kidneys of human infants obtained at autopsy. This paper presents the results of immunofluorescence by this method and shows that antibody to human collagen becomes fixed to its antigen, collagen, in the human renal glomerular and tubular basement membranes. Materials and MethodsPurified acid-soluble collagen prepared from human skin, rat tail tendons, chicken leg tendons, or carp swim bladder, and rabbit antisera to these collagens were prepared by methods previously reported (2, 3). The technique of complement fixation for determining the titer of

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confidence: 99%

Demonstration by Immunofluorescence of the Fixation of Perfused Antibody to Human Collagen in Human Kidney

Rothbard

Watson

1967

The Journal of Experimental Medicine

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“…It should be mentioned that Schaub [26] reported a rise in hydroxy proline content in rat kidneys beginning from the 5th month of age. On the other hand Bonting et al [27] found no change with age in hydroxyproline concentration in human glomerular tufts in spite of the thickening of the basement membranes. The authors explain this finding on the assumption that all elements in the glomeruli increase in weight during growth.…”

Section: Resultsmentioning

confidence: 92%

Age-Related Changes in Renal Connective Tissue of Rats

Allalouf

Wasserman

et al. 1969

Gerontology

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“…Initial studies suggested the presence of collagen in normal human glomerular ECM by the high amount of glycine, hydroxyproline, and hydroxylysine in glomerular extracts[ 30 , 66 - 68 ]. Relative protein quantification confirms an abundant amount of type I, type IV, and type VI collagen in human glomerular ECM.…”

Section: Normal Composition Of the Human Gbm And Mesangial Matrixmentioning

confidence: 99%

Biochemical composition of the glomerular extracellular matrix in patients with diabetic kidney disease

Adeva‐Andany¹,

Carneiro-Freire²

2022

WJD

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In the glomeruli, mesangial cells produce mesangial matrix while podocytes wrap glomerular capillaries with cellular extensions named foot processes and tether the glomerular basement membrane (GBM). The turnover of the mature GBM and the ability of adult podocytes to repair injured GBM are unclear. The actin cytoskeleton is a major cytoplasmic component of podocyte foot processes and links the cell to the GBM. Predominant components of the normal glomerular extracellular matrix (ECM) include glycosaminoglycans, proteoglycans, laminins, fibronectin-1, and several types of collagen. In patients with diabetes, multiorgan composition of extracellular tissues is anomalous, including the kidney, so that the constitution and arrangement of glomerular ECM is profoundly altered. In patients with diabetic kidney disease (DKD), the global quantity of glomerular ECM is increased. The level of sulfated proteoglycans is reduced while hyaluronic acid is augmented, compared to control subjects. The concentration of mesangial fibronectin-1 varies depending on the stage of DKD. Mesangial type III collagen is abundant in patients with DKD, unlike normal kidneys. The amount of type V and type VI collagens is higher in DKD and increases with the progression of the disease. The GBM contains lower amount of type IV collagen in DKD compared to normal tissue. Further, genetic variants in the α3 chain of type IV collagen may modulate susceptibility to DKD and end-stage kidney disease. Human cellular models of glomerular cells, analyses of human glomerular proteome, and improved microscopy procedures have been developed to investigate the molecular composition and organization of the human glomerular ECM.

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Quantitative Histochemistry of the Nephron. Vi. Hydroxyproline in the Human Glomerulus*

Cited by 20 publications

References 9 publications

Demonstration by Immunofluorescence of the Fixation of Perfused Antibody to Human Collagen in Human Kidney

Demonstration by Immunofluorescence of the Fixation of Perfused Antibody to Human Collagen in Human Kidney

Age-Related Changes in Renal Connective Tissue of Rats

Biochemical composition of the glomerular extracellular matrix in patients with diabetic kidney disease

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