Quantitative human chorionic gonadotropin analysis

Fifteen immunoassay kits for serum hCG(-β) measurements have been compared for their analytical characteristics and clinical usefulness in tumour monitoring. Three out of 13 radioimmunoassays and two sandwich hCG immunoassays represent the ‘one-component’ techniques for intact hCG detection since they are based on antiserum raised against intact hCG. Within this group, method-comparison slopes ranged between 0·149 and 0·374. The remaining 10 radioimmunoassays all apply antiserum against hCG-β and are designated ‘two (or more)–component’ assays since they detect both intact hCG and the free β-subunit (‘total hCG’). For this reason these assays are of potential value for tumour monitoring. Additional criteria for this are: (1) the expression of assay results in terms of 1st IRP-hCG instead of the unstructured use of the 2nd IS or the 1st IRP-hCG-β; (2) documentation of cross-reactivities especially for the free β-subunits of hCG and hLH which are almost completely lacking; (3) establishment of the minimum detectable dose in the presence of normo-to-hypergonadotrophic hLH levels in serum (the ‘clinical sensitivity’) to allow the follow-up of tumour regression especially in the low-dose region. Method-comparison analysis for these assays revealed regression slopes between 0·001 and 0·873.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Quantitative human chorionic gonadotropin analysis

Cited by 2 publications

References 7 publications

Covert cross reactants in a two-site immunoassay studied with monoclonal antibodies

Covert cross reactants in a two-site immunoassay studied with monoclonal antibodies

Comparison of the Analytical Characteristics and Clinical Usefulness in Tumour Monitoring of Fifteen hCG(-β) Immunoassay Kits

Contact Info

Product

Resources

About