Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas

Babczyńska, Agnieszka; Wilczek, Grażyna; Szulińska, Elżbieta; Franiel, Izabella

doi:10.1016/j.ecoenv.2011.05.019

Cited by 24 publications

(6 citation statements)

References 30 publications

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“…Variables such as life stage, sex and seasonal diet changes influence HM concentrations within a species collected from a contaminated habitat [29,30]. Among species, several traits including capacity for dispersal, prey choice, ability to store or excrete HMs and seasonal occurrence can influence concentrations of HMs found within natural enemies [31][32][33][34][35][36][37]. For example, seasonality was found to influence Pb contamination of sheet web spiders (Araneae: Linyphiidae) in vacant lots and urban farms; those collected in early June contained significantly greater concentrations of Pb than those present in July or August ( Figure 2D).…”

Section: Natural Enemies In a Contaminated Landscapementioning

confidence: 99%

“…HM exposure can influence the production of metallothioneins and enzymes involved in detoxification, including carboxyesterases (CarE), catalases (CAT), glutathiones (GSH), glutathione-S-transferases (GST), gluthione reductase (GR), superoxide dismutases (SOD), and selenium-dependent (GPOX) and selenium-independent (GSTPX) glutathione peroxidases [36, 50, 57-59, 63-64, 69]. HM exposure frequently leads to greater detoxification enzyme activity; however, variation exists both within and between species, often varying by sex [36,50]. Induction of detoxification enzymes above normal levels may have fitness consequences for arthropods as energetic demands shift from maintenance and reproduction.…”

Section: Detoxification Immunity and Energy Costsmentioning

confidence: 99%

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Influence of heavy metal contamination on urban natural enemies and biological control

Gardiner

Harwood

2017

Current Opinion in Insect Science

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Urban agriculture is increasing worldwide. A history of contamination within urban landscapes may negatively impact the biota necessary for sustainable crop production, including arthropod natural enemies. This investigation revealed that heavy metal contamination can influence the composition of natural enemy communities and exposure can have reproductive, developmental, immunological and behavioral impacts on predators and parasitoids. Natural enemies exposed to heavy metals typically live shorter lives, take longer to develop and exhibit a reduced reproductive potential. Further, they may incur significant energy costs though the production of detoxification enzymes. This is a new and relatively unexplored area for biological control research, with important implications for our understanding of urban agricultural food web interactions.

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Section: Natural Enemies In a Contaminated Landscapementioning

confidence: 99%

Section: Detoxification Immunity and Energy Costsmentioning

confidence: 99%

Influence of heavy metal contamination on urban natural enemies and biological control

Gardiner

Harwood

2017

Current Opinion in Insect Science

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“…C. salei , a wandering spider (Ctenidae), has been the subject of neurological, physiological and behavioural studies over many decades [e.g. [6-8]]. More recently, this species has also been used for evo-devo studies [e.g.…”

Section: Introductionmentioning

confidence: 99%

The embryonic development of the central American wandering spider Cupiennius salei

Wolff

Hilbrant

2011

Front Zool

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BackgroundThe spider Cupiennius salei (Keyserling 1877) has become an important study organism in evolutionary and developmental biology. However, the available staging system for its embryonic development is difficult to apply to modern studies, with strong bias towards the earliest developmental stages. Furthermore, important embryonic events are poorly understood. We address these problems, providing a new description of the embryonic development of C. salei. The paper also discusses various observations that will improve our understanding of spider development.ResultsConspicuous developmental events were used to define numbered stages 1 to 21. Stages 1 to 9 follow the existing staging system for the spider Achaearanea tepidariorum, and stages 10 to 21 provide a high-resolution description of later development. Live-embryo imaging shows cell movements during the earliest formation of embryonic tissue in C. salei. The imaging procedure also elucidates the encircling border between the cell-dense embryo hemisphere and the hemisphere with much lower cell density (a structure termed 'equator' in earlier studies). This border results from subsurface migration of primordial mesendodermal cells from their invagination site at the blastopore. Furthermore, our detailed successive sequence shows: 1) early differentiation of the precheliceral neuroectoderm; 2) the morphogenetic process of inversion and 3) initial invaginations of the opisthosomal epithelium for the respiratory system.ConclusionsOur improved staging system of development in C. salei development should be of considerable value to future comparative studies of animal development. A dense germ disc is not evident during development in C. salei, but we show that the gastrulation process is similar to that in spider species that do have a dense germ disc. In the opisthosoma, the order of appearance of precursor epithelial invaginations provides evidence for the non-homology of the tracheal and book lung respiratory systems.

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“…With the exception of the nematode Caenorhabditis elegans and the snails Helix pomatia and Cornu aspersum (formerly Helix aspersa or Cantareus aspersus), few ground living invertebrates are characterized with respect to the presence and turnover of metalloproteins. Cadmium thioneins are induced in springtails (Hensbergen et al 1999), earthworms (Yamamura et al 1981, Bengtsson et al 1992, Stürzenbaum et al 2001, molluscs (Dallinger et al 1997(Dallinger et al , 2004, and spiders (Babczyńska et al 2011b), and copper, zinc, and lead thioneins have been found in earthworms (Bengtsson et al 1992), spiders (Babczyńska et al 2011b) and molluscs (Dallinger et al 1997, Palacios et al 2011. Analyses of the molecular structures have revealed the presence of two to three homometallic or mixed metalcontaining isoforms, binding cadmium, copper, zinc or combinations of two of them .…”

Section: Introductionmentioning

confidence: 96%

PIXE-electrophoresis shows starving collembolan reallocates protein-bound metals

Bengtsson

Pallon²,

Nilsson³

et al. 2015

Ecotoxicology

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One of multiple functions of metalloproteins is to provide detoxification to excess metal levels in organisms. Here we address the induction and persistence of a range of low to high molecular weight copper- and zinc binding proteins in the collembolan species Tetrodontophora bielanensis exposed to copper- and zinc-enriched food, followed by a period of recovery from metal exposure, in absence and presence of food. After 10 days of feeding copper and zinc contaminated yeast, specimens were either moved to ample of leaf litter material from their woodland stand of origin or starved (no food offered). The molecular weight distribution of metal binding proteins was determined by native polyacryl gel electrophoresis. One gel was stained with Comassie brilliant blue and a duplicate gel dried and scanned for the amount of copper and zinc by particle-induced X-ray emission. Specimens exposed to copper and recovered from it with ample of food had copper bound to two groups of rather low molecular weight proteins (40-50 kDa) and two of intermediate size (70-80 kDa). Most zinc in specimens from the woodland stand was bound to two large proteins of about 104 and 106 kDa. The same proteins were holding some zinc in metal-exposed specimens, but most zinc was found in proteins <40 kDa in size. Specimens recovered from metal exposure in presence of ample of food had the same distribution pattern of zinc binding proteins, whereas starved specimens had zinc as well as copper mainly bound to two proteins of 8 and 10 kDa in size. Thus, the induction and distribution of copper- and zinc-binding proteins depend on exposure conditions, and the presence of low molecular weight binding proteins, characteristic of metallothioneins, was mainly limited to starving conditions.

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Quantitative immunodetection of metallothioneins in relation to metals concentration in spiders from variously polluted areas

Cited by 24 publications

References 30 publications

Influence of heavy metal contamination on urban natural enemies and biological control

Influence of heavy metal contamination on urban natural enemies and biological control

The embryonic development of the central American wandering spider Cupiennius salei

PIXE-electrophoresis shows starving collembolan reallocates protein-bound metals

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