Quantitative iTRAQ‐based proteomic analysis of rice grains to assess high night temperature stress

Zhang, Hongyu; Liu, Gang; Zhou, Huiwen; He, Chao; Liao, Jiang-Lin; Huang, Yongji

doi:10.1002/pmic.201600365

Cited by 71 publications

(49 citation statements)

References 52 publications

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“…High temperature induces different degrees of elongation growth of det1 , cop1 , and hy5 mutants (Delker et al 2014), suggesting that high temperature affects hypocotyl elongation partially through DET‐, COP1‐ and HY5‐mediated signaling. A very recent proteomic study reveals that after night‐heat stress, the abundance of CSN3 was increased significantly in heat‐tolerant rice lines but decreased dramatically in the heat‐sensitive rice lines at early milky stage (Zhang et al 2017), implying that CSN may contribute to the heat tolerance of rice (Zhang et al 2017). However, the evidence supporting a role of CSN in regulating high temperature response in Arabidopsis is still lacking.…”

Section: Csn Plays Pleiotropic Roles In Various Developmental Processmentioning

confidence: 99%

COP9 signalosome: Discovery, conservation, activity, and function

Qin

et al. 2020

JIPB

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The COP9 signalosome (CSN) is a conserved protein complex, typically composed of eight subunits (designated as CSN1 to CSN8) in higher eukaryotes such as plants and animals, but of fewer subunits in some lower eukaryotes such as yeasts. The CSN complex is originally identified in plants from a genetic screen for mutants that mimic light-induced photomorphogenic development when grown in the dark. The CSN complex regulates the activity of cullin-RING ligase (CRL) families of E3 ubiquitin ligase complexes, and play critical roles in regulating gene expression, cell proliferation, and cell cycle. This review aims to summarize the discovery, composition, structure, and function of CSN in the regulation of plant development in response to external (light and temperature) and internal cues (phytohormones).

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Section: Csn Plays Pleiotropic Roles In Various Developmental Processmentioning

confidence: 99%

COP9 signalosome: Discovery, conservation, activity, and function

Qin

et al. 2020

JIPB

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“…Global warming-associated increases in temperature, particularly during the night, are detrimental to rice production and yield, as over-warm nighttime temperatures increase the rate of grain growth and decrease grain growth duration [26,27]. The molecular mechanisms associated with the response of rice to HNT have been widely investigated in the past decade, and several of the genes, proteins, metabolites, and probable gene-regulatory networks involved in the rice HNT response have been explored [21][22][23]. However, the primary genetic elements and specific molecular mechanisms conferring rice heat tolerance remain unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we reported that the genes involved in electron transport and oxidation equilibrium in plant cells were disrupted by short-term extreme HNT, leading to changes in hydrogen ion concentrations in the mitochondrial and cellular matrices, which triggered and regulated downstream stress-response genes and proteins involved in photosynthesis, nucleotide catabolism, and the Sadenosylmethionine (SAM) cycle, resulting in abnormal grain-filling in rice [21][22][23]. However, the primary genetic elements regulating the gene and/or protein activity in the network upon exposure of rice to HNT need to be explored.…”

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confidence: 99%

Cytosine methylation in the promoter region of genes involved in cellular oxidation equilibrium pathways affects rice heat tolerance

Zhang

et al. 2020

Preprint

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Background High temperatures are detrimental to rice yield and quality, especially at night. Due to the predicted increases in global warming, it is imperative that crops are able to adapt to high night temperatures (HNTs) in the near future. DNA methylation is a potential mechanism for controlling gene activity and cellular phenotype under adversely environment without changing nucleotide sequence.Results Here, we reported that few CG and CHG contexts but CHH context in rice genome was strongly induced by HNT to occur cytosine methylation variation between two coisogenic rice strains with significant difference in heat tolerance. Methylation variations occurred mainly on successive cytosines in the promoter or downstream regions of transcription factors and transposon elements. In contrast to the heat-sensitive rice strain, two basal transcriptional factor TFIID subunit 11 and mediator of RNA polymerase II transcription subunit 31 were fully demethylated in the downstream 358-359 bp and 2-60 bp under HNT in the heat-tolerant strain, respectively. Various genes involved in ABA-related reactive oxygen species (ROS) equilibrium pathways, including the pentatricopeptide repeat domain gene PPR (LOC_Os07g28900) and the homeobox domain gene homeobox (LOC_Os01g19694), were induced by HNT to occur methylation variation on successive cytosines in the gene promoter regions of the heat-tolerant strain. Varidation among the typical heat-tolerant group and heat-sensitive group of rice germplasms, methylation rate of the cytosines in gene promoter region for gene PPR was higher and gene expression was suppressed in the heat-sensitive group, comparing to the heat-tolerant group.Conclusions The CHH context in rice genome was the main context type to occur cytosine methylation variation under HNT between the heat-sensitive and heat-tolerant rice strains. Methylation in the promoter regions of the genes related to abscisic acid-related oxidation and ROS scavenging contributes to rice heat tolerance. These findings provide bases to explain the molecular mechanisms behind rice heat tolerance.

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“…HT during grain-filling period accelerates the growth rate of endosperm and causes grain chalkiness [6,7]. Intensive investigation revealed that HT exposure impaired the biosynthesis and deposition of starch in rice grains as a result of HT-induced downregulation of various genes involving in starch biosynthesis [8][9][10]. Nevertheless, little information was available on the metabolic mechanism underlying the effect of N fertilizer on grain chalk occurrence.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analysis reveals the metabolic pathways of grain chalky occurrence in response to nitrogen topdressing for rice

et al. 2019

Preprint

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Background Grain chalkiness is a highly undesirable quality trait that adversely affects consumer acceptability and lowers the market value. Except for the genotypic variation among different rice cultivars, this chalkiness is readily influenced by application of chemical fertilizers, particularly nitrogen (N) at the late growth stage of rice. However, it is not fully clear on the molecular mechanism underlying the formation of grain chalkiness caused by N fertilization. Results Using OM052 with the chalkiness rate over 90% as material, experiment was conducted with two N fertilizer topdressing treatments at the panicle initiation stage, including 108 kg N ha-1 applied (N+) and without N topdressing to rice as a control (N0). N+ significantly enhanced the area of endosperm chalkiness and the degree of grain chalkiness. The amylose content of rice grains under N+ was lower than that under N0, while the opposite trend was true for N+–induced change in grain protein content. Proteomic analysis found that a total of 198 proteins differentially expressed between N+ and N0, including 9 up-regulated proteins and 189 down-regulated proteins for rice plants imposed to N+. Approximately 31.3% of these differentially expressed proteins (DEPs) involved in N metabolism (protein synthesis, folding, degradation and storage, and amino acid synthesis and catabolism), 21.7% of DEPs belonged to carbohydrate metabolism (glycolysis, tricarboxylic acid cycle, pentose phosphate pathway, fermentation and starch metabolism), and 17.7% of DEPs participated in stress/defense regarding redox homeostasis and removal of aldehydes. Conclusion Multiple metabolic pathways in the developing caryopsis were affected by N topdressing, especially the mitochondrial respiration, sucrose-to-starch metabolism and N metabolism. The insufficient supply of ATP energy as a result of the significantly lowered mitochondrial respiration induced by N+ regime inhibits the sucrose-to-starch metabolism and starch biosynthesis in developing grains, and is strongly responsible for grain chalk formation under N topdressing.

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Quantitative iTRAQ‐based proteomic analysis of rice grains to assess high night temperature stress

Cited by 71 publications

References 52 publications

COP9 signalosome: Discovery, conservation, activity, and function

COP9 signalosome: Discovery, conservation, activity, and function

Cytosine methylation in the promoter region of genes involved in cellular oxidation equilibrium pathways affects rice heat tolerance

iTRAQ-based quantitative proteomic analysis reveals the metabolic pathways of grain chalky occurrence in response to nitrogen topdressing for rice

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