Quantitative Label-free Determination of Thrombin Using a Chemically-modified M13 Virus-electrode Interface

Shin, Jae Hwan; Yang, Hyo Jeong; Kim, Ji Eun; Yang, Jin Chul; Park, Jin‐Young; Park, Jong Pil

doi:10.1007/s12257-022-0361-9

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Currently, cutting-edge sensor platforms, including surfaced-enhanced Raman scattering (SERS), electrochemical (EC) sensor systems, and fluorescence sensor systems, have emerged as powerful and promising tools. − These methods have shown their efficacy in achieving sensitive and convenient detection of influenza virus by employing various bioreceptors such as antibodies, aptamers, and peptides as recognition elements. Among the bioreceptors, peptides have emerged as a particularly promising choice by playing an important role in detecting numerous biological and chemical molecules, including viruses, food allergy, disease biomarkers, and small molecules, in recent years. − Furthermore, peptides can be chemically synthesized with ease, allowing modifications that enhance stability, binding affinity, and specificity to overcome the disadvantages of antibodies such as complex production and fragility in harsh environments.…”

Section: Introductionmentioning

confidence: 99%

Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus

Kim,

Jeong,

Hwang

et al. 2023

ACS Appl. Mater. Interfaces

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Influenza viruses are known to cause pandemic flu outbreaks through both inter-human and animal-to-human transmissions. Therefore, the rapid and accurate detection of such pathogenic viruses is crucial for effective pandemic control. Here, we introduce a novel sensor based on affinity peptide-immobilized hydrogel microspheres for the selective detection of influenza A virus (IAV) H3N2. To enhance the binding affinity performance, we identified novel affinity peptides using phage display and further optimized their design. The functional hydrogel microspheres were constructed using the drop microfluidic technique, employing a structure composed of natural (chitosan) and synthetic (poly(ethylene glycol) diacrylate and PEG 6 kDa) polymers with the activation of azadibenzocyclooctyne for the subsequent click chemistry reaction. The binding peptide-immobilized hydrogel microsphere (BP-Hyd) was characterized by field emission scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy and exhibited selective detection capability for the IAV H3N2. To capture the detected IAV H3N2, a Cy3-labeled IAV hemagglutinin antibody was utilized. By incorporating the affinity peptide with hydrogel microspheres, we achieved quantitative and selective detection of IAV H3N2 with a detection limit of 1.887 PFU mL–1. Furthermore, the developed suspension sensor exhibited excellent reproducibility and showed reusability potential. Our results revealed that the BP-Hyd-based fluorescence sensor platform could be feasibly employed to detect other pathogens because the virus-binding peptides can be easily replaced with other peptides through phage display, enabling selective and sensitive binding to different targets.

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