Quantitative mass spectrometry as a tool for nutritional proteomics

Moresco, James J.; Dong, Meng

doi:10.1093/ajcn/88.3.597

Cited by 13 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent notable reviews report on the increasing utility of proteomics to various aspects of nutrition research . In particular, nontargeted, global proteomics could be used to identify novel targets of response to a nutritional intervention at the tissue or serum/plasma level, thus forming the basis to a ‘hypothesis‐generating’ strategy .…”

Section: Introductionmentioning

confidence: 99%

Sex‐specific vitamin D effects on blood coagulation among overweight adults

Al-Daghri

Alokail

Manousopoulou

et al. 2016

Eur J Clin Investigation

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Sex‐specific vitamin D effects on blood coagulation among overweight adults

Al-Daghri

Alokail

Manousopoulou

et al. 2016

Eur J Clin Investigation

View full text Add to dashboard Cite

show abstract

“…4–8 Quantitation of these discrete modifications can be used to determine relative expression levels in living systems. Mass spectrometry (MS) is commonly employed to identify the components of pharmacological or biological mixtures, 9–14 and is used to identify thousands of peptides originating from protein digests of whole cells, biological fluids, tissues and organisms. 15–29 …”

Section: Introductionmentioning

confidence: 99%

Direct Quantitation of Peptide Mixtures without Standards Using Clusters Formed by Electrospray Ionization Mass Spectrometry

2009

View full text Add to dashboard Cite

In electrospray ionization mass spectrometry, ion abundances depend on a number of different factors, including analyte surface activity, competition between analytes for charge, analyte concentration, as well as instrumental factors, including mass-dependent ion transmission and detection. Here, a novel method for obtaining quantitative information about solution-phase concentrations of peptide mixtures is described and demonstrated for five different peptide mixtures with relative concentrations ranging from 0.05% to 50%. In this method, the abundances of large clusters containing anywhere from zero to thirteen impurity molecules are measured and directly related to the relative solution-phase concentration of the peptides. For clusters containing ~15 or more peptides, the composition of the clusters approaches the statistical value indicating that these clusters are formed nonspecifically and that any differences in ion detection or ionization efficiency are negligible at these large cluster sizes. This method is accurate to within ~20% or better, even when the relative ion intensities of the protonated monomers can differ by over an order of magnitude compared to their solution-phase concentrations. Although less accurate than other quantitation methods that employ internal standards, this method does have the key advantages of speed, simplicity, and the ability to quantitate components in solution even when the identity of the components are unknown.

show abstract

“…In the case of a Nutrigenomic study, the proteome provides a "picture" of the impact of specific bioactive nutrients and diets in a certain organism, tissue or cell in a particular moment. Thus, the importance of Proteomics as a tool to understand the effect of the diet on health has already been recognised by several authors [18,[85][86][87][88][89][90], however, up to now the application of Proteomics in Nutrigenomics is still rather limited, being the number of review papers higher than research papers [91].…”

Section: Advances In Proteomicsmentioning

confidence: 99%

Advances in Nutrigenomics research: Novel and future analytical approaches to investigate the biological activity of natural compounds and food functions

García‐Cañas

Simó

León

et al. 2010

Journal of Pharmaceutical and Biomedical Analysis

104

View full text Add to dashboard Cite

In recent years, nutrition research has moved from classical epidemiology and physiology to molecular biology and genetics. Following this trend, Nutrigenomics has emerged as a novel and multidisciplinary research field in nutritional science that aims to elucidate how diet can influence human health. It is already well known that bioactive food compounds can interact with genes affecting transcription factors, protein expression and metabolite production. The study of these complex interactions requires the development of advanced analytical approaches combined with bioinformatics. Thus, to carry out these studies Transcriptomics, Proteomics and Metabolomics approaches are employed together with an adequate integration of the information that they provide. In this article, an overview of the current methodologies and a thorough revision of the advances in analytical technologies and their possibilities for future developments and applications in the field of Nutrigenomics is provided.

show abstract

Quantitative mass spectrometry as a tool for nutritional proteomics

Cited by 13 publications

References 49 publications

Sex‐specific vitamin D effects on blood coagulation among overweight adults

Sex‐specific vitamin D effects on blood coagulation among overweight adults

Direct Quantitation of Peptide Mixtures without Standards Using Clusters Formed by Electrospray Ionization Mass Spectrometry

Advances in Nutrigenomics research: Novel and future analytical approaches to investigate the biological activity of natural compounds and food functions

Contact Info

Product

Resources

About