Quantitative Measurement of Long Range Nuclear Overhauser Enhancement

Modified GOESY in the Analysis of Disaccharide Conformation

“…This selective inversion during the mixing time, suggested by Olejniczak et al (42), can in theory be carried out a number of times in order to ensure complete cancellation. In practice, however, a previous report suggests that imperfect inversion of the magnetization as well as relaxation during the selective pulses limits the number of inversions which may be performed while still obtaining adequate sensitivity (27).…”

“…The second approach is similar to the 2D QUIET-NOESY experiment (25,26) as well as the 1D experiment reported by Harris et al (27) for the suppression of spin diffusion.…”

12 NMR Spectroscopic methods

“…A method for measuring nuclear Overhauser enhancements free of spin diffusion effects has been developed. 24 Spin diffusion effects tend to distort the size of the measured NOE, and is most severe for molecules in the slow tumbling regime (ot c b 1). The new pulse sequence that accomplishes this combines excitation sculpting with the use of two semiselective inversion pulses to suppress spin diffusion.…”