Quantitative microanalysis of matrix elements in biological samples by MeV proton scattering

Hult, Mikael; Themner, K.

doi:10.1016/0168-583x(90)90349-y

Cited by 4 publications

(2 citation statements)

References 6 publications

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“…The cross-sections were cut ca 10 mm from the tip with 20 #m thickness of samples. The cross-sections were placed on a circular holder covered by a plastic foil, a polyimide film (Kapton TM), with good resistance against loss of elements induced by radiation (Hult & Themner 1990). To avoid movement of elements in samples during the preparation the temperature was maintained below -5°C in the fresh samples.…”

Section: Sample Preparationmentioning

confidence: 99%

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Influence of aluminium and nitrogen on uptake and distribution of minerals in beech roots (Fagus sylvatica)

Bengtsson

1992

Vegetatio

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Beech seedlings (Fagus sylvatica L. provenance V~stra Torup) were grown in nutrient solution at low pH (4.2) and exposed to A1CI3 and different concentrations of nitrogen. The effects ofA1C13 and nitrogen on uptake of C a 2 + (45Ca) and H2PO4 (32p) in roots of intact beech were studied. Crossections of roots were analyzed with respect to element concentrations, by use of Mikro-PIXE (particle induced X-ray emission). The distribution of A1, Ca, P, Mg, K, and S was analysed. The experiments were designed to evaluate the effects of aluminium on localization of elements in plant root tissue.Aluminium reduced the concentration of Ca in plants and increased that of K. High nitrogen levels in the solution further decreased the concentration of Ca in the roots. Aluminium (1.0 mM) effectively reduced the Ca 2 ÷ (45Ca) uptake in short time experiments. Aluminium accumulated in high concentration (up to 500 #mol/g dry weight in areas of 30 x 30/~m 2) on the root surface, epidermis and outer layers of cortex. Corresponding areas had an extremely low Ca concentration (ca 5/~mol/g dry weight) which could be harmful for regulation of mineral uptake and development of roots.It is concluded that the calcium concentration in roots was reduced by aluminium. The combination with high nitrogen levels further reduced calcium changing the mineral balance which could result in deficiency conditions of calcium in the root.

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Section: Sample Preparationmentioning

confidence: 99%

“…From the emission spectra it was possible to quantify different elements. For further information about PIXEanalysis see Malmqvist (1986 (Hult & Themner 1990). The hydrogen fraction in plant material was approximated to 6~o of dry weight.…”

Section: Particle Induced X-ray Emission-analysis (Pixe)mentioning

confidence: 99%