Investigation of Ni hyperaccumulation by true elemental imaging

Przybyłowicz, W.J.; Pineda, C.A.; Prozesky, V.M.; Mesjasz‐Przybyłowicz, Jolanta

doi:10.1016/0168-583x(95)00445-9

Cited by 45 publications

(15 citation statements)

References 16 publications

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“…The celluar compartmentation of Zn and As in the leaf epidermis was found in Thlaspi caerulescens and Pteris vittata, respectively (Küpper et al, 1999;Lombi et al, 2002a). In nickel-hyperaccumulation plants Senecio coronatus and Alyssum lesbiacum, nickel was preferentially accumulated in the epidermis or epidermal trichomes of the leaves (Przybylowicz et al, 1995;Kramer et al, 1997). However, cadmium and zinc in mesophyll were higher than that in leaf epidermis in hyperaccumulator Arabidopsis halleri (Küpper et al, 2000).…”

Section: Cu Distribution Charactersmentioning

confidence: 88%

SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

Shi

Chen

Huang

et al. 2004

Micron

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Section: Cu Distribution Charactersmentioning

confidence: 88%

SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

Shi

Chen

Huang

et al. 2004

Micron

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“…Such studies provide the opportunity to identify sites of detoxiWcation and storage of metal complexes. Investigations into the distribution of nickel within the leaves have shown that nickel is accumulated in the epidermis (Mesjasz-Przybylowicz et al 1994;Przybylowicz et al 1995;Heath et al 1997), and more speciWcally in epidermal cells away from guard cells (Psaras et al 2000). Ni was distributed preferentially in the epidermal cells, most likely the vacuoles, of the leaves and stems of the hyperaccumulators A. lesbiacum, A. bertolonii and Thlaspi goesingense (Küpper et al 2001).…”

Section: Introductionmentioning

confidence: 98%

Cellular and subcellular compartmentation of Ni in the Eurasian serpentine plants Alyssum bracteatum, Alyssum murale (Brassicaceae) and Cleome heratensis (Capparaceae)

Asemaneh

Ghaderian

Crawford

et al. 2006

Planta

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This study investigated the cellular and subcellular compartmentation of Ni in the Eurasian serpentine species Alyssum murale, Alyssum bracteatum and Cleome heratensis and a non-serpentine population of A. murale (as a control) grown in hydroponic culture. Plant growth responses and Ni uptake clearly revealed the higher Ni tolerance of serpentine plants than the non-serpentine plants. Serpentine A. murale and A. bracteatum grew better at elevated (0.01 mM) Ni in the nutrient solution, supporting the view that the Ni hyperaccumulators have a higher requirement for Ni than normal plants. Low shoot Ni content of C. heratensis in response to the high Ni treatments indicated that this species employs an avoidance strategy for Ni tolerance. Energy-dispersive X-ray microanalysis showed that Ni was highly concentrated in the cell walls and cell lumen, most likely the vacuoles, of leaf epidermis of A. murale and A. bracteatum rather than in the mesophyll cells. EDX spectra from leaves of the non-serpentine A. murale suggested that Ni accumulated in both epidermal and mesophyll cells but not in the epidermal cell walls. Growth reduction and Ni toxicity in plants of the non-serpentine A. murale could be due to accumulation of Ni in the lumen of leaf mesophyll cells. Our data suggest that cellular and subcellular compartmentation are both possible mechanisms for Ni tolerance employed by the serpentine A. murale and A. bracteatum.

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“…Harv. (Asteraceae) (Przybylowicz et al, 1995), Senecio anomalochrous Hilliard (Mesjasz- Przybylowicz et al, 2001), Stackhousia tryonii Bailey (Stackhousiaceae) (Bhatia et al, 2003), and Sebertia acuminata Pierre ex Baill. (Sapotaceae) (Perrier et al, 2004), has recently begun.…”

Section: Introductionmentioning

confidence: 99%

Nickel distribution in the hyperaccumulatorAlyssum serpyllifoliumDesf. spp. from the Iberian Peninsula

Fuente

Rodríguez

Díez-Garretas

et al. 2007

Plant Biosystems - An International Journal Dealing with all As

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The relative concentration and distribution of nickel (Ni) in vegetative tissues (leaves, stems and trichomes) and reproductive organs (seeds) was studied using energy-dispersive X-ray microanalysis (EDXS) and scanning electron microscopy (SEM) in two previously studied Ni-hyperaccumulator subspecies of Alyssum serpyllifolium Desf. growing naturally in ultramafic soils of the Iberian Peninsula: A. serpyllifolium ssp. lusitanicum Dudley & P. Silva and A. serpyllifolium ssp. malacitanum Rivas Goday ex G. Ló pez. Both taxa showed that Ni accumulates preferentially in the leaves, exhibiting a homogeneous distribution on both epidermis surfaces. The highest Ni concentrations were found inside the epidermal cells and at the base of trichome stalks. Ni accumulation in seeds was lower than in the vegetative organs. The location of Ni in these plants allows us to postulate that its accumulation is a protection mechanism against external stress.

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Investigation of Ni hyperaccumulation by true elemental imaging

Cited by 45 publications

References 16 publications

SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens

Cellular and subcellular compartmentation of Ni in the Eurasian serpentine plants Alyssum bracteatum, Alyssum murale (Brassicaceae) and Cleome heratensis (Capparaceae)

Nickel distribution in the hyperaccumulatorAlyssum serpyllifoliumDesf. spp. from the Iberian Peninsula

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