Quantitative plasmalipoprotein electrophoresis. Correction for the difference in dye uptake by the lipoprotein fractions

Dyerberg, Jørn; Hjörne, N.

doi:10.1016/0009-8981(70)90133-6

Cited by 24 publications

(10 citation statements)

References 11 publications

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“…The separated lipoproteins are stained with a lipid-specific Sudan black stain. This quantitative method is in good accordance with the reference ultracentrifugation results and has been found to be reliable for clinical use by previous studies (9,10).…”

Section: Lipids Lipoprotein and Ogtt Measurementssupporting

confidence: 71%

“…This discrepancy indicates that the direct LDL-C measurement or the direct VLDL-C measurement was flawed. The use of the homogeneous LDL-C and HDL-C measurement as a reference method is appropriate, given the known inaccuracies of these methods (9,10). The performance of directly measured VLDL-C by electrophoresis is reliable, however, the technology-demand and the cost of this method is relative high (10,28).…”

Section: Discussionmentioning

confidence: 99%

“…The use of the homogeneous LDL-C and HDL-C measurement as a reference method is appropriate, given the known inaccuracies of these methods (9,10). The performance of directly measured VLDL-C by electrophoresis is reliable, however, the technology-demand and the cost of this method is relative high (10,28). In the present study, we also discovered a more suitable constant Y value of 5.7 for ethnic Chinese people by a simplified equation for those who cannot adopt the modified equation we provided (instead of the conventional Friedewald constant of 5).…”

Section: Discussionmentioning

confidence: 99%

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Validation of the Estimation of Low-density Lipoprotein Cholesterol by the Modified Friedewald Equation in Ethnic Chinese Adults Living in Taiwan

Hsu

Chen

et al. 2015

Intern. Med.

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Objective The Friedewald equation has been used as the standard formula for the estimation of very lowdensity lipoprotein cholesterol (VLDL-C) to calculate the serum levels of low-density lipoprotein cholesterol (LDL-C). However, the usefulness of this formula in non-Caucasians has been challenged in recent years. The aim of this study was to assess the validity of the conventional and modified Friedewald equation in an ethnic Chinese adult population. Methods We prospectively recruited 938 subjects from the Lipid Clinic of the National Taiwan

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Section: Lipids Lipoprotein and Ogtt Measurementssupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Validation of the Estimation of Low-density Lipoprotein Cholesterol by the Modified Friedewald Equation in Ethnic Chinese Adults Living in Taiwan

Hsu

Chen

et al. 2015

Intern. Med.

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“…It has bcen reported that different lipids, either in pure form from commercial sources (Schjcidc et al, 1963) or extracted from human plasma (Dyerberg and Hjorne, 1970), have different affinities for Sudan black B. Cholesteryl esters have the highest affÉnity, followed, in order, by triglycerides, phospholipids, and fatty acids; free cholesterol binds minimally to the stain. Furthermore, unsaturated lipids have a higher affinity for the stain than saturated lipids (Schjcidc et al, 1963).…”

Section: Affinity Of Lipids To Sudan Black Bmentioning

confidence: 98%

Method for quantitating cholesterol in subfractions of serum lipoproteins separated by gradient gel electrophoresis

et al. 1988

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Extensive heterogeneity in particle size distribution of serum lipoproteins of baboons was resolved by a procedure that combined Sudan black B prestaining, polyacrylamide gradient gel electrophoresis (GGE), and quantitative densitometry. Each densitometric scan represented a continuous distribution of the relative amount of cholesterol in a serum sample, as a function of the lipoprotein particle size. For analytical purposes, each scan was divided into 12 fractions, representing 12 particle size ranges. The relationship between the estimated cholesterol concentrations in the summed GGE/densitometric fractions corresponding to very low-density lipoproteins (VLDL) + low-density lipoproteins (LDL) and those corresponding to high-density lipoproteins (HDL) and concentrations measured by the heparin-Mn2+ precipitation/enzymatic procedure was linear over a broad range. However, a systematic overestimation of HDL cholesterol concentration and an underestimation of VLDL + LDL cholesterol concentration was apparent. Therefore, correction factors were developed for adjusting the estimates of VLDL + LDL and HDL cholesterol concentrations obtained by the GGE/densitometric method. This analytical method is rapid, repeatable, economical, and useful for genetic and dietary research in which cholesterol concentrations in multiple particle size ranges of lipoproteins must be measured in large numbers of samples. It also is adaptable to immunoblotting procedures for detecting the distribution of specific apolipoproteins among the size-resolved lipoproteins.

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“…The need for a simple quantitative method for plasma lipoprotein determination was stressed in previous papers describing a method based on agarose gel electrophoresis with mathematical correction for the difference in dye uptake by the different lipoproteins (10,11).…”

mentioning

confidence: 99%

Plasma Lipid and Lipoprotein Levels in a Danish Population

Dyerberg

Hjörne

1972

Journal of Internal Medicine

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A study of the plasma lipid and lipoprotein concentrations has been carried out in 373 persons (179 women and 194 men) including 56 obese persons (24 women and 32 men) and 26 women treated with sex hormones, mainly for contraceptive purposes. The technique for quantitative lipoprotein electrophoresis described by the authors in previous papers was used. The technique was compared with other methods and found reliable. Increasing lipid and lipoprotein level with age was demonstrated; but while this increase continued in women, the increase in men ceased at about the age of 45. The levels of triglycerides and pre‐β‐lipoproteins were found to be higher in men than in women, while phospholipids and α‐lipoproteins were higher in women. Cholesterol and β‐lipoproteins were higher in women aged 11–20 years and over 50 years than in men in the corresponding age groups. Higher levels of triglycerides and pre‐β‐lipoproteins were found in obese than in non‐obese persons regardless of sex. An observation not described before was a lower α‐lipoprotein concentration in obese than in non‐obese women. No significant influence of contraceptive sex hormone intake on the lipid and lipoprotein levels could be demonstrated.

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Quantitative plasmalipoprotein electrophoresis. Correction for the difference in dye uptake by the lipoprotein fractions

Cited by 24 publications

References 11 publications

Validation of the Estimation of Low-density Lipoprotein Cholesterol by the Modified Friedewald Equation in Ethnic Chinese Adults Living in Taiwan

Validation of the Estimation of Low-density Lipoprotein Cholesterol by the Modified Friedewald Equation in Ethnic Chinese Adults Living in Taiwan

Method for quantitating cholesterol in subfractions of serum lipoproteins separated by gradient gel electrophoresis

Plasma Lipid and Lipoprotein Levels in a Danish Population

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