Quantitative prediction of a functional ingredient in apple using Raman spectroscopy and multivariate calibration analysis

Tsuyama, Shinsaku; Taketani, Akinori; Murakami, Takeharu; Sakashita, Michio; Miyajima, Saki; Ogawa, T.; Wada, Satoshi; Maeda, Hayato; Hanada, Yasutaka

doi:10.1007/s00340-021-07639-0

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…In these studies, the concentration of procyanidin was monitored using high-performance liquid chromatography, which is not a cost- or time-effective approach. Recently, apple procyanidin was monitored using Raman spectroscopy and multivariate calibration analysis [ 62 ]. Although this study detected procyanidin with non-destructed apple, procyanidin in apple juice was not measured.…”

Section: Resultsmentioning

confidence: 99%

The Flavonoid Molecule Procyanidin Reduces Phase Separation in Model Membranes

Yoda

2022

Membranes

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Procyanidin extracted from fruits, such as apples, has been shown to improve lipid metabolization. Recently, studies have revealed that procyanidin interacts with lipid molecules in membranes to enhance lipid metabolism; however, direct evidence of the interaction between procyanidin and lipid membranes has not been demonstrated. In this study, the phase behaviors and changes in the membrane fluidity of cell-sized liposomes containing apple procyanidin, procyanidin B2 (PB2), were demonstrated for the first time. Phase separation in 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/cholesterol ternary membranes significantly decreased after the addition of PB2. The prospect of applying procyanidin content measurements, using the results of this study, to commercial apple juice was also assessed. Specifically, the PB2 concentrations were 50%, 33%, and 0% for pure apple juice, 2-fold diluted apple juice, and pure water, respectively. The results of the actual juice were correlated with PB2 concentrations and phase-separated liposomes ratios, as well as with the results of experiments involving pure chemicals. In conclusion, the mechanism through which procyanidin improves lipid metabolism through the regulation of membrane fluidity was established.

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Section: Resultsmentioning

confidence: 99%

The Flavonoid Molecule Procyanidin Reduces Phase Separation in Model Membranes

Yoda

2022

Membranes

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“…It can also be observed that after dispersing 0.03 g/L concentration of CNT nanofluid in MO, bands at 1156 cm −1 , 1375 cm −1 , and 1599 cm −1 are also missing compared to MO and MO with 0.02 g/L CNT concentration. The band at 1156 cm −1 is attributed to in-phase (C-C) stretching vibrations of the polyene chain [22], while bands at 1375 cm −1 and 1599 cm −1 attributes to the (H-C-C/O) bending mode and (C=C) aromatic stretching mode, which describes the characteristic of diamond and graphite. If higher concentrations of amorphous carbon are present, those two peaks can be observed respectively [23].…”

Section: Multiple Peak Fitting Analysismentioning

confidence: 99%

Raman Spectroscopy Characterization of Mineral Oil and Palm Oil with Added Multi-Walled Carbon Nanotube for Application in Oil-Filled Transformers

et al. 2022

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This century is experiencing a generation of nanotechnologies that makes use of the remarkable properties of nanofluids in applications such as electrical systems, industrialization, and others. In this paper, mineral oil (MO) and palm oil (PO), with multi-walled carbon nanotube (CNT), have been synthesized for use in oil-filled transformer applications. This research aims to use Raman characterization to assess the feasibility of CNT nanofluids samples at 0.02 g/L and 0.03 g/L concentrations. The chemical structure bonding that exists in the Raman band between 700 cm−1 and 3100 cm−1 regions is identified and analyzed, accordingly. After baseline removal and normalization, the precision band location and intensity of oil samples are fitted with a Gaussian profile. It was discovered that the peak at ~1440 cm−1 has the highest intensity for six oil samples, which is attributed to the (C–H) methylene scissors vibration of the CH2 group. Based on the FWHM profile and integrated area under the curve of PO, it was discovered that CNT contributes to the structural stability defect of PO. Principal component analysis (PCA) is also used in this study to classify different samples based on chemical composition and identify the spectral characteristics with the highest degree of variability.

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“…These techniques for the interpretation of spectral data are largely based on the development of regression models, also known as multivariate calibration 11–15 . Previously, our group reported the quantitative prediction of procyanidins in apples using Raman spectroscopy with multivariate calibration analysis 16 . In this prior work, partial least‐squares (PLS) regression, a type of chemometric method, was combined with Raman spectroscopy to statistically extract information from spectral data with multivariate information while eliminating interference related to autofluorescence and extraneous peaks.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of eating quality of white rice using Raman spectroscopy with multivariate analysis

Yamada,

Saito,

Maeda

et al. 2023

J Raman Spectroscopy

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The present study evaluated the eating quality of uncooked white rice using Raman spectroscopy, operating with a 532 nm excitation beam, in conjunction with multivariate calibration. This analysis was performed to establish correlations between features of the spectra and the results of conventional sensory tests with the aim of establishing calibration models. The accuracy of each model was confirmed based on the associated coefficient of determination (R2), root‐mean‐square error of calibration and root mean square error of prediction values. Models based on partial least‐squares regression provided reasonable predictions (R2 > 0.8) for the eating quality of white rice. In addition, the components affecting total evaluation were evaluated using the variable importance in projection technique, and the results showed that starch, amylose, amino acids, glucans, carbohydrates, and proteins are all crucial in terms of determining the quality of white rice. This newly developed method is likely to be suitable for the evaluation of rice quality and, in principle, could also be applied to the assessment of other foods.

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Quantitative prediction of a functional ingredient in apple using Raman spectroscopy and multivariate calibration analysis

Cited by 9 publications

References 37 publications

The Flavonoid Molecule Procyanidin Reduces Phase Separation in Model Membranes

The Flavonoid Molecule Procyanidin Reduces Phase Separation in Model Membranes

Raman Spectroscopy Characterization of Mineral Oil and Palm Oil with Added Multi-Walled Carbon Nanotube for Application in Oil-Filled Transformers

Evaluation of eating quality of white rice using Raman spectroscopy with multivariate analysis

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