Quantitative proteomic analysis ofRhodococcus ruberresponsive to organic solvents

Abstract

Rhodococcus ruber with organic tolerance has potential applications in biotransformation and bioremediation. To explore the possible organic tolerance mechanism, the response of R. ruber SD3 to toluene and phenol was investigated using a quantitative proteomics approach with isobaric tag for relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry. A total of 362 and 488 differentially expressed proteins were identified in the toluene treatment group and the phenol treatment group as compared to the control group, respectively. Functional annotation and metabolic pathway enrichment showed that transporter, degradation pathway and two-component system were closely related to organic solvent tolerance of R. ruber SD3. The quantitative real-time polymerase chain reaction experiment indicated the mRNA levels of stress proteins with an increased expression of 3.23 times upon toluene stress as compared to the control. The expression of 4-nitrophenol 2-monooxygenase in the phenol treatment group was $123 times higher than the counterpart in the control group. The study revealed the possible tolerance mechanism of R. ruber SD3 to organic solvents stress and provided some potential targets for the engineering of R. ruber SD3 to improve its organic solvent tolerance.

Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency

“…All the previous proteomic investigations, which were performed on Rhodococcus , only highlighted its catabolic behavior towards different organic compounds viz . Fluoranthene 14 , toluene and phenol 15 , propane 16 , triacylglycerol 17 , 4,4′-Dithiodibutyric Acid 18 , benzoate 19 and polyaromatic compounds 20 . In this perspective, the present study for the first time unravels its proteomic response towards the abiotic stress and identified a protein biomarker for low-temperature nitrogen deficient conditions.…”

Multi-omics analysis on seasonal variations of the biofilm microbial community in a full-scale pre-denitrification biofilter

“…The enrichment of the quorum sensing pathway implies its role in the adaptation of bio lm microbiome to seasonal variations. The two-component system is a fundamental stimulus-response coupling mechanism that enables microorganisms to sense and respond to changes in environmental conditions (Kuang et al, 2018). Following seasonal variations, some proteins involved in the two-component system pathway were differentially regulated.…”