Quantitative proteomics profiling of spermatozoa and seminal plasma reveals proteins associated with semen quality in Bos indicus bulls

Ashwitha, A; Ramesha, K. P.; Ramesh, Poornima; Kootimole, Chinmaya Narayana; Devadasan, M. Joel; Ammankallu, Shruthi; Jeyakumar, Sakthivel; Kumaresan, A.; Veerappa, Vedamurthy G.; Das, Debanand; Prasad, T. S. Keshava

doi:10.1016/j.jprot.2022.104794

Cited by 11 publications

(5 citation statements)

References 56 publications

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“…TMT-based quantitative proteomics analysis reveals that the protein expression level of SOD1 protein in the frozen group was significantly lower than that in the fresh group, and the protein expression level of NDUFS8 protein was significantly higher in frozen group [ 21 ]. Proteins such as OAZ3, GPx4, and GSTM3 whose upregulation leads to reduced motility were upregulated in the spermatozoa of high ERR bulls, the regulation of ACE, a negative regulator of sperm motility was upregulated in both the spermatozoa and seminal plasma of high ERR bulls [ 22 ]. HSPA5 could regulate sperm capacitation and slow down apoptosis by comparative proteomic identification of capacitated and non-capacitated sperm of Yanbian Yellow Cattle [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Wang,

Liu,

Cao

et al. 2023

Proteome Sci

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Background Proteins related to sperm motility and sperm morphology have an important impact on sperm function such as metabolism, motility and fertilisation etc. An understanding of the key proteins related to semen quality in Niangya yaks would help to provide support for breeding. However, the key proteins that affect semen quality in Niangya yaks remain unclear. Methods Herein, we applied tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC‒MS/MS) to analyze the expression levels of sperm proteins in groups of high- and low-quality semen from Niangya yaks. And fifteen differentially expressed proteins (DEPs) were randomly selected for expression level validation by parallel reaction monitoring (PRM). Results Of the 2,092 quantified proteins, 280 were identified as DEPs in the high-quality group versus the low-quality group. Gene Ontology (GO) analysis revealed that in terms of biological pathways, the DEPs were mainly involved in metabolic processes, cell transformation processes, and single organism metabolic processes. In terms of cell composition, the DEPs were mainly located in the cell membrane, organelle, molecular complex. In terms of molecular functions, the most abundant functions of the DEPs were catalytic activity, binding activity, transport activity, and enzyme regulation activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were mainly involved in the cytokine and cytokine receptor interaction, notch signaling pathway, lysine biosynthesis, renal function-related protein and proteasome pathway. From protein-protein interaction (PPI) analysis of DEPs involved in important pathways, 6 related proteins affecting the semen quality of Niangya yaks were identified. And the results of the PRM and TMT analysis were consistent. Conclusions The differential sperm proteomic analysis of high- and low-quality semen from Niangya yaks, revealed 6 proteins (PSMC5, PSMD8, PSMB3, HSP90AA1, UGP2 and HSPB1), were mainly concentrated in energy production and metabolism, might play important roles in semen quality, which could serve as candidates for the selection and breeding of Niangya yaks.

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Section: Discussionmentioning

confidence: 99%

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Wang,

Liu,

Cao

et al. 2023

Proteome Sci

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“…Sperm fertility is the capability of sperm to fertilize and activate oocytes to support embryonic development. Both are essential factors that can affect male fertility [ 2 ]. The reproductive selection of bull fertility by the breeding soundness examination (BSE) method shows that the production and quality of sperm produced are not optimal, although bulls have passed BSE selection, and sperm concentration and motility are even very low [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality

Baharun,

Rahmi,

Kardaya

et al. 2023

J Adv Vet Anim Res

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Objective: The present study analyzed the seminal plasma proteome and possible relationships between proteins and semen quality in azoospermic and normal Simmental bulls. Materials and Methods: Fresh semen plasma samples from the Lembang Artificial Insemination Center were used for this study, including one bull (76´ ejaculate) with very poor semen quality/ azoospermia (poor fresh semen/infertile; PFS) and three bulls with normal semen quality (normal fresh semen; NFS) for proteomic analysis using a pooled system (NFS-Stud) (60´ ejaculate). The only males obtained with very low quality or azoospermia (PFS) had sperm motility of <10% (one head). Bulls with azoospermic conditions produce fresh semen without sperm or with very little sperm concentration. A total of 109 proteins were identified in the seminal plasma of Simmental bulls analyzed using liquid chromatography-mass spectrometry. Bioinformatics analysis was used to explore total protein, expression, function, and protein mechanism in the seminal plasma of Simmental bulls. Results: The results showed that the seminal plasma proteins expressed in NFS bulls include ELSPBP1, SIL1, HSPA13, angiotensin-1 covering enzyme, and CRISP1. On the other hand, B2M, C3, CFB, venin-2, and cathepsin S contribute significantly to PFS. The NFS bull proteins play important roles in sperm capacitation, protein transport, sperm motility, spermatogenesis, immune toler¬ance, and fertilization, while the PFS proteins perform apoptotic and antigen pathway functions. Conclusion: There is an interaction between proteins in the seminal plasma of males with poor semen quality (PFS) and cases of infertility (azoospermia) that cause a decrease in sperm quality in PFS bulls.

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“…Therefore, the determination of the function, localization, and PPI of specific fertility-related proteins differentially expressed between samples from fertile and infertile men is required [18]. As a rule, the spermatozoa and seminal plasma proteomes are investigated separately for this purpose [19,20]. In turn, this leads to an increase in the cost and timing of the proteomic analysis, which limits its large-scale use in clinical applications.…”

Section: Introductionmentioning

confidence: 99%

The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening

Shkrigunov,

Zgoda,

Klimenko

et al. 2023

Biomedicines

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Problems with the male reproductive system are of both medical and social significance. As a rule, spermatozoa and seminal plasma proteomes are investigated separately to assess sperm quality. The current study aimed to compare ejaculate proteomes with spermatozoa and seminal plasma protein profiles regarding the identification of proteins related to fertility scores. A total of 1779, 715, and 2163 proteins were identified in the ejaculate, seminal plasma, and spermatozoa, respectively. Among these datasets, 472 proteins were shared. GO enrichment analysis of the common proteins enabled us to distinguish biological processes such as single fertilization (GO:0007338), spermatid development (GO:0007286), and cell motility (GO:0048870). Among the abundant terms for GO cellular components, zona pellucida receptor complex, sperm fibrous sheath, and outer dense fiber were revealed. Overall, we identified 139 testis-specific proteins. For these proteins, PPI networks that are common in ejaculate, spermatozoa, and seminal plasma were related to the following GO biological processes: cilium movement (GO:0003341), microtubule-based movement (GO:0007018), and sperm motility (GO:0097722). For ejaculate and spermatozoa, they shared 15 common testis-specific proteins with spermatogenesis (GO:0007283) and male gamete generation (GO:0048232). Therefore, we speculated that ejaculate-based proteomics could yield new insights into the peculiar reproductive physiology and spermatozoa function of men and potentially serve as an explanation for male infertility screening.

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Quantitative proteomics profiling of spermatozoa and seminal plasma reveals proteins associated with semen quality in Bos indicus bulls

Cited by 11 publications

References 56 publications

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality

The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening

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