Quantitative semen parameters of Atlantic cod (Gadus morhua) and their physiological relationships with sperm activity and morphology

Butts, Ian A.E.; Rideout, Rick M.; Burt, K.; Samuelson, S.; Lush, L.; Litvak, Matthew K.; Trippel, Edward A.; Hamoutene, Dounia

doi:10.1111/j.1439-0426.2010.01545.x

Cited by 18 publications

(12 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…) and Atlantic cod (Butts et al . ). In this sense, it is known that ATP alone is not a strong indicator of sperm motility by itself, and other metabolites such as ADP or CrP should be taken into account (Dzyuba et al .…”

Section: Fish Sperm Motility: a Useful Tool For Multidisciplinary Stumentioning

confidence: 97%

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Gallego

Asturiano

2018

Reviews in Aquaculture

View full text Add to dashboard Cite

Fish sperm motility is nowadays considered the best biomarker for the quality of fish spermatozoa, and sperm motion parameters from more than 300 fish species have been reported in more than 1500 scientific articles covering a wide range of topics, from molecular biology to ecology. The most studied topics have been (i) the sperm storage (involving both the use of chilled-storage protocols for shortterm periods and sperm cryopreservation techniques for long-term storage), (ii) the sperm physiology (fathom in the spermatozoa activation process and the whole propulsion machinery of the sperm cells) and (iii) the broodstock management (covering aspects such as rearing conditions, dietary requirements or hormonal induction treatments). In addition, other aquaculture and ecological topics, such as (iv) the knowledge of the breeding cycle of the species, (v) the phenomenon of the sperm competition and (vi) ecotoxicological studies for the evaluation of aquatic environments, have also been approached from the evaluation of sperm motion performance. Therefore, fish sperm motility assessment can serve as a potential tool for aquaculture and ecological purposes, covering key topics of fundamental and applied research. This review gives an overview of the major research areas in which fish sperm motility has been applied successfully.

show abstract

Section: Fish Sperm Motility: a Useful Tool For Multidisciplinary Stumentioning

confidence: 97%

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Gallego

Asturiano

2018

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…Level of decline of ATP after sperm activation differs among species. For example, ATP content significantly decreased at 10 s after sperm activation in Dicentrarchus labrax (Dreanno et al 1999a), but at 30 s after activation in bluegill sunfish (Lepomis macrochirus Rafinesque, 1819) (Burness et al 2005), G. morhua (Butts et al 2010b), and Eurasian perch (Perca fluviatilis L., 1758) (A. Hatef and S.M.H.…”

Section: Discussionmentioning

confidence: 97%

“…Once sperm are activated, motility and velocity decrease rapidly (see review by Alavi and Cosson 2006;Cosson 2010) owing to the depletion of ATP content Butts et al 2010b). This phenomenon leads to changes in beating frequency of flagellum, wavelength, wave amplitude, and number of propagating waves (Ishijima et al 1998;Cosson et al 2008;Alavi et al 2009aAlavi et al , 2009bCosson 2010), which determine the sperm velocity (Alavi et al 2009a;Cosson 2010).…”

Section: Introductionmentioning

confidence: 99%

Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)

et al. 2011

View full text Add to dashboard Cite

Spermatozoon of Atlantic halibut (Hippoglossus hippoglossus (L., 1758)) is uniflagellated, lacks an acrosome, and is differentiated into a head, midpiece, and flagellum. There are two to five mitochondria in the midpiece, as well as proximal and distal centrioles. The flagellum consisted of 9 + 2 microtubules surrounded by plasma membrane, which is extended at the proximal part of the flagellum owing to the presence of vacuoles. After sperm activation in seawater, sperm motility and velocity decreased from 98.4% ± 3.4% and 170.3 ± 8.9 mmÁs -1 at 15 s after sperm activation to 4.8% ± 4.7% and 9.2 ± 8.9 mmÁs -1 at 120 s after sperm activation, respectively. ATP content (nmolÁL -1 ATP per 10 8 spermatozoa) significantly decreased at 60 s after sperm activation (5.9 ± 1.5) compared with at 0 and 30 s after sperm activation (14.9 ± 1.5 and 14.5 ± 1.5, respectively). Beating waves propagated along the full length of the flagellum after sperm activation, whereas waves were restricted to the proximal section during the latter motility period. Wave amplitude significantly decreased at 45 s after sperm activation, but wavelength did not differ. The present study showed associations among sperm morphology, ATP content, flagellar wave parameters, and sperm velocity, which could be used in comparative spermatology.Résumé : Le spermatozoïde du flétan atlantique (Hippoglossus hippoglossus (L., 1758)) est uniflagellé, sans acrosome et différencié en tête, corps médian et flagelle. Il y a deux à cinq mitochondries dans le corps médian, ainsi que des centrioles proximaux et distaux. Le flagelle consiste en 9 + 2 microtubules entourés d'une membrane plasmatique qui se prolonge dans la partie proximale du flagelle à cause de la présence de vacuoles. Après l'activation des spermatozoïdes dans l'eau de mer, la motilité et la vitesse des spermatozoïdes diminuent respectivement de 98,4 % ± 3,4 % et de 170,3 ± 8,9 mmÁs -1 à 15 s après l'activation à 4,8 % ± 4,7 % et 9,2 ± 8,9 mmÁs -1 à 120 s après l'activation. Le contenu en ATP (nmolÁL -1 ATP par 10 8 spermatozoïdes) diminue significativement 60 s après l'activation (5,9 ± 1,5) par comparaison à 0 et 30 s après l'activation (respectivement 14,9 ± 1,5 et 14,5 ± 1,5). Les ondulations de battement se propagent sur toute la longueur du flagelle après l'activation, mais sont restreintes à la partie proximale durant la période de motilité subséquente. L'amplitude des ondulations diminue significativement à 45 s après l'activation, mais leur longueur d'onde ne change pas. Notre étude met en lumière des associations entre la morphologie des spermatozoïdes, leur contenu en ATP, les variables ondulatoires des flagelles et la vitesse des spermatozoïdes qui pourraient servir en spermatologie comparée.[Traduit par la Rédaction]

show abstract

“…However, if there is limited time available to fertilize an egg, then selection should favor faster-swimming spermatozoa. This will most likely be at the expense of longevity because there are limited amounts of energy reserves available for spermatozoa motility (Perchec et al 1995, Burness et al 2005, Butts et al 2010. Ultimately, these gamete strategies provide an evolutionary challenge for fertilization for both males and females, as evidenced by counter-strategies employed.…”

Section: Introductionmentioning

confidence: 99%

Fertilization strategies for winter flounder: effects of spermatozoa density and the duration of gamete receptivity

Butts¹,

Roustaian²,

Litvak³

2012

Aquat. Biol.

View full text Add to dashboard Cite

Winter flounder is one of the most commonly used models for studying fish biology in North America; however little is known about their reproductive ecology, especially during the spawning event. The objectives of this research were to determine the optimal number of spermato zoa required to fertilize eggs and to explore how long spermatozoa (30 to 240 s post-activation) and eggs (30 to 7680 s post-activation) are receptive to fertilization after exposure to seawater. We conducted experiments using gametes from wild-caught fish and measured fertilization success by examining eggs at 5 to 6 d post-fertilization. On average 34 038 sperm cells per egg were required to fertilize 81.3% of the eggs. Duration after spermatozoa activation had an effect on the proportion of eggs fertilized (F 3, 6.69 = 338.38; p < 0.0001; mixed-model ANOVA). At 30 s post-spermatozoa activation, 98% of the eggs were fertilized. After 60 s, a significant decrease in fertilization success was detected. Duration after egg exposure to seawater had an effect on the proportion of eggs fertilized (F 8,16 = 19.89; p < 0.0001;. For all trials (30 to 1920 s), the percentage of eggs fertilized ranged from 61 to 90%. A significant decrease to 11% occurred at 3840 s after egg exposure. This area of research has particular importance for our understanding of reproductive strategies, evolutionary challenges, reproductive potential and recruitment. In addition, examining sperm-egg interactions provides information important to management of living and frozen-thawed gene banks. KEY WORDS: Marine fish · Reproduction · Sperm · Egg · Fisheries · Aquaculture · Segmented regressionResale or republication not permitted without written consent of the publisher Aquat Biol 16: 115-124, 2012 gian coastal origin starts in August and continues for about 6 months and the number of pre-spawning spermatogonia present in the testes essentially determines the numbers produced each season (Almeida et al. 2008). In addition, since most fishes are external fertilizers and the medium in which the spermatozoa is released can be turbulent, i.e. oceans and rivers (Trippel 2003, Faria et al. 2006), there may not be an 'unlimited' number of spermatozoa available to fertilize eggs released by females in these environments.Spermatozoa longevity and egg receptivity -the length of time an egg is receptive to be fertilized by a sperm cell -also impact the success of a fertilization event (Kjørsvik & Lønning 1983, Trippel & Morgan 1994, Butts et al. 2009). For instance, if female mate choice is important, then eggs may be receptive for a short period of time to reduce the chance of fertilization by non-desirable males. In contrast, if eggs are receptive for longer periods of time then the probability of fertilization by multiple males should in crease; this may be important in situations when the probability of spermatozoa to egg encounters are low (Trippel 2003). If spermatozoa are viable for longer periods of time, then the potential of contacting and fertilizing an egg ...

show abstract

Quantitative semen parameters of Atlantic cod (Gadus morhua) and their physiological relationships with sperm activity and morphology

Cited by 18 publications

References 46 publications

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Fish sperm motility assessment as a tool for aquaculture research: a historical approach

Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)

Fertilization strategies for winter flounder: effects of spermatozoa density and the duration of gamete receptivity

Contact Info

Product

Resources

About