Quantitative single dilution liquid phase blocking ELISA for sero-monitoring of foot-and-mouth disease in India

Sharma, Gaurav Kumar; Mahajan, Sumit; Matura, Rakesh; Subramaniam, Saravanan; Mohapatra, Jajati K.; Pattnaik, Bramhadev

doi:10.1016/j.biologicals.2015.03.003

Cited by 8 publications

(14 citation statements)

References 12 publications

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“…These strains were used in the vaccine formulation. Antibody titres were quantified by SdLPBE as described previously (Sharma et al., ). In brief, test serum samples were diluted to 1 : 32 and mixed individually with equal volume of pre‐titrated inactivated whole virus antigen of the three serotypes making the final dilution of serum to 1 : 64.…”

Section: Methodsmentioning

confidence: 99%

“…Proficiency in estimating the antibody titres by various state level diagnostic laboratories was determined as described previously (Sharma et al., ). About 10% of the total samples tested at AICRP laboratories were selected at random and re‐tested at the central laboratory, Mukteswar.…”

Section: Methodsmentioning

confidence: 99%

“…Herd immunity offers protection to the vaccinated animals (direct benefits), while indirectly provides the benefits to the unvaccinated ones by reducing the virus transmission rate (Fine et al., ). Since August 2010, cattle and buffalo (bovines) population of 221 districts of the country are regularly vaccinated at 6 months interval under government funded National FMD control programme (FMDCP) (Pattnaik et al., ; Sharma et al., ), while in rest of the districts, vaccination is performed once in a year, although the remaining districts will be included under the FMDCP in the coming years (Pattnaik et al., ). Although length and size of FMD outbreaks have been significantly reduced with occasional isolated incidences due to systematic vaccinations in these areas, an abrupt increase in FMD incidences was recorded in the year 2013 (Subramaniam et al., ; Sharma et al., ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Herd Immunity Against Foot-and-Mouth Disease Under Different Vaccination Practices in India

Sharma

Mahajan

Matura

et al. 2016

Transbound Emerg Dis

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A systematic vaccination programme is ongoing in India to control the three prevailing serotypes (A, O, Asia1) of foot-and-mouth disease (FMD) virus. Under the programme, more than 120 million bovine (term bovine applicable to both cattle and buffalo in this study) population of 221 of the 666 districts in the country are being bi-annually vaccinated with trivalent vaccine since 2010. Although clinical disease has reduced in these districts because of the systematic vaccinations, an abrupt increase in the number of FMD cases was recorded in 2013. Hence, a longitudinal field study was conducted in the year 2014 to estimate the serological herd immunity level in bovines, the impact of systematic vaccinations and field efficacy of the vaccines used. Serum samples (n = 115 963) collected from 295 districts of the 18 states of the country were analysed to estimate antibody titres against structural proteins of the three serotypes. The efficacy of the vaccine was demonstrated in the control group (group-D) where animals of the group were identified by ear tags for the purpose of repeated sampling after vaccination. Progressive building of the herd immunity in the field after systematic vaccination was demonstrated. The mean antibody titre against the serotypes O, A and Asia1 was estimated as log 1.93 (95% CI 1.92-1.93), 2.02 (2.02-2.02) and 2.02 (2.02-2.02), respectively, in the states covered under the control programme. However, in other states herd immunity was significantly low [mean titre log 1.68 (95% CI 1.67-1.69), 1.77 (1.76-1.78) and 1.85 (1.84-1.86) against the three serotypes]. Inverse relationship between the herd immunity and FMD incidences was observed the states following different vaccination practices. The study helped in demarcation of FMD risk zones in the country with low herd immunity. Estimation of herd immunity kinetics in the field helped in refining the vaccination schedule under the control programme.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Herd Immunity Against Foot-and-Mouth Disease Under Different Vaccination Practices in India

Sharma

Mahajan

Matura

et al. 2016

Transbound Emerg Dis

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“…To overcome this problem, a single-dilution LPB-ELISA was previously developed to measure the FMDV-specific antibody titer in serum. This method is based on a linear regression curve generated with reference standards to extrapolate the antibody titers of the sera tested (72,73).…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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“…Secondly, how to accurately differentiate FMDV infected from immunized animals is an important challenge. Normally, the serological methods for FMDV antibody level evaluation are all based on SPs and whole virus, including virus neutralization test, 10 liquid phase blocking enzyme linked immunosorbent assay (LPB-ELISA), 11 solid-phase competition ELISA (SPCE), 12 while ELISAs based on NSPs are normally used to discriminate infected animals from vaccinated animals. [13][14][15][16] Recently, the immunochromatographic strip was be successfully used in various elds due to its specicity, sensitivity, rapidity, low cost and adaptability for eld detection and high sample throughput.…”

Section: Introductionmentioning

confidence: 99%

A gold nanoparticle strip for simultaneously evaluating FMDV immunized antibody level and discriminating FMDV vaccinated animals from infected animals

et al. 2019

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Quantitative single dilution liquid phase blocking ELISA for sero-monitoring of foot-and-mouth disease in India

Cited by 8 publications

References 12 publications

Herd Immunity Against Foot-and-Mouth Disease Under Different Vaccination Practices in India

Herd Immunity Against Foot-and-Mouth Disease Under Different Vaccination Practices in India

Advances in the Diagnosis of Foot-and-Mouth Disease

A gold nanoparticle strip for simultaneously evaluating FMDV immunized antibody level and discriminating FMDV vaccinated animals from infected animals

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