Quantitative Studies on Antithrombin

Wilson, Sloan J.

doi:10.1001/archinte.1942.00200160098008

Cited by 15 publications

(4 citation statements)

References 26 publications

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“…For this reason it was not considered probable that the anticoagulant was a fibrinogen antagonist. Determination of the antithrombin activity of this plasma by the method described by Wilson (7) showed that there was no increase in antithrombin compared to that of normal plasma. Addition of protamine 1 in quantities of 0.001 to 0.1 mgm.…”

Section: Resultsmentioning

confidence: 95%

Properties of an Anticoagulant Found in the Blood of a Hemophiliac

Munro

1946

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 95%

Properties of an Anticoagulant Found in the Blood of a Hemophiliac

Munro

1946

J. Clin. Invest.

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“…In all tubes, the clotting times were within the same range as the normal controls (5 to 7 minutes). Determinations of plasma antithrombin (14) in four bleeder dogs gave results of 90 to 120 per cent of normal control dogs; this is within the range of normal. Recent evidence indicates that faulty conversion of prothrombin to thrombin may be due to a deficiency of one or more cofactors which accelerate prothrombin conversion in the presence of optimal calcium and an excess of thromboplastin.…”

Section: The Clotting Anomalymentioning

confidence: 52%

“…The clotting tests were carried out at 28°C. Quantitafive determination of plasma antithrombin was made by the method of Wilson (14).…”

mentioning

confidence: 99%

Canine Hemophilia

Graham

Buckwalter

Hartley

et al. 1949

Journal of Experimental Medicine

105

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Field, Rickard, and H u t t (1, 2) have reported the occurrence of a sex-llnked hemorrhagic disease in male dogs similar to hemophilia in man. The chief symptoms were due to subcutaneous hematomas and hemarthroses. Deformities frequently occurred. Most of the pups affected with the disease died during the first 12 weeks of life. Of 17 affected males described, none were reared to maturity. The female stock, heterozygous for the disease, was turned over to this laboratory so that a controlled breeding program could be instituted, and a more extensive investigation of the clotting defect could be made. Our studies of the affected male progeny indicate that the clotting defect is identical with t h a t found in human hemophilia. Repeated transfusions with whole blood or plasma alleviate the hemorrhagic phenomena, and permit growth of affected dogs to m a t u r i t y practically free of deformities. Materials and MethodsThe male bleeder dogs used in this study were born and reared in our kennel The dams were known transmitters of the disease and of Irish setter stock. The sires were normal males selected without respect to breed.The clotting time of whole blood was determined by two methods: (a) a modified Lee and White method (3), in which two 10 X 75 ram. dry glass tubes were used for each test, and (b) the silicone method, in which needles, syringes, and tubes coated with methylchlorsilane (4) were used. The method for determining the clotting time of platelet-rich and plateletpoor citrated plasmas has been described (5). Platelet-rich plasmas were obtained by centrifugation of citrated whole blood for 5 minutes at 450 g. Platelet-poor plasmas were prepared by centrifugation for 2 hours at 13,000 g in silicone-treated glassware.Bleeding time was determined by a modification of Duke's method (6). The site of incision in one series of observations was the mucous membrane of the lip (1); in another series the inner hairless aspect of the pinna (tragus of the ear) was used. Incisions were approximately 2 ram. deep. On the lip the incisions were 2 to 5 mm. long, on the ear 5 to 7 mm.The platelets in whole blood were counted by a modification of Nygaard's method (7),

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“…Various colloids also speed up the reaction (38). It has been shown that a certain amount of thrombin disappears with fibrin formation (19,32,46). Later, in this paper, data will be presented to show that this is incidentally due to adsorption of thrombin on fibrin and is not a part of the basic mechanics of fibrinogen and thrombin interactions.…”

Section: A System Of Interactionsmentioning

confidence: 91%

Multiple Protein Interactions as Exhibited by the Blood-clotting Mechanism.

Seegers¹

1947

J. Phys. Chem.

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There is now emerging, from the researches of the past century, a tangible concept of a whole system of protein interactions involved in the blood-clotting mechanism. An undertaking of these reactions will lead to new viewpoints in protein chemistry. From the advances and contributions already made, it is apparent that the techniques of physical chemistry in conjunction with quantitative measurements of protein reactivity will be drawn upon heavily for the elucidation of the problems involved. It is the purpose of this paper to organize the existing information as briefly as possible, to show the relationships of the components of the system, and to incorporate new contributions from our labora-1 Presented at the Twentieth National Colloid Symposium, which was held at Madison, Wisconsin, May 28-29, 194C.

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Quantitative Studies on Antithrombin

Cited by 15 publications

References 26 publications

Properties of an Anticoagulant Found in the Blood of a Hemophiliac

Properties of an Anticoagulant Found in the Blood of a Hemophiliac

Canine Hemophilia

Multiple Protein Interactions as Exhibited by the Blood-clotting Mechanism.

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