Quantitative studies on cell differentiation during morphogenesis of the cellular slime mold Dictyostelium discoideum

Hayashi, Moriaki; Takeuchi, Ikuo

doi:10.1016/0012-1606(76)90153-6

Cited by 81 publications

(24 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sum of these two populations of stained cells, about 23-26%, is in good agreement with independent counts of stained cells in total slug tissue (25.6 + 3.1%; 11 slugs). This value is also in good agreement with the estimate of Hayashi and Takeuchi (1976) of about 24% nonprespore cells in slugs stained with a fluorescently labeled antibody to prespore cells. These values are somewhat higher than a more recent estimate of 19% nonprespore cells (Hayashi and Takeuchi, 1981).…”

Section: Proportions Of Anterior Anterior-like and Posterior Cellssupporting

confidence: 89%

See 1 more Smart Citation

Fate and regulation of anterior-like cells in Dictyostelium slugs

Sternfeld

David

1982

Developmental Biology

181

103

View full text Add to dashboard Cite

Section: Proportions Of Anterior Anterior-like and Posterior Cellssupporting

confidence: 89%

“…Comparisons of the proportion of anterior and posterior tissue have been based both on volume estimations (Bonner, 1957) and cell counting (Hayashi and Takeuchi, 1976). Here, to determine the proportion of anterior-like cells, it was necessary to count the cells.…”

Section: Proportions Of Anterior Anterior-like and Posterior Cellsmentioning

confidence: 99%

Fate and regulation of anterior-like cells in Dictyostelium slugs

Sternfeld

David

1982

Developmental Biology

181

103

View full text Add to dashboard Cite

“…For determination of the ratio of prespores to total cells, disaggregated cells were fixed, air-dried on coverglasses and stained with fluorescein isothiocyanate conjugated antibody produced against spores of Dictyostelium mucoroides, as described by HAYASHI and TAKEUCHI (10). For examination of the dedifferentiation of prespore cells (21), cells disaggregated from cell masses were washed, resuspended in BSS and placed on coverglasses.…”

Section: °Cmentioning

confidence: 99%

A Mutant of Dictyostelium Discoideum Capable of Differentiating Without Morphogenesis

Ishida

1980

Dev Growth Differ

View full text Add to dashboard Cite

A mutant which is capable of differentiating into spores and stalk cells without forming a cell aggregate was isolated from the cellular slime mould, Dictyosteliurn discoideum. The mutant stopped developing at various stages, before formation of mature fruits, and the cells differentiated into s p o r a and stalk cells at whichever stage the develop-nent stopped. Unaggregated cells also differentiated into spores or stalk cells, depending on the culture conditions; differentiation into spores predominated in nutrient rich medium, while differentiation into stalk cells predominated in nutrient poor medium. The ratio of spores to stalk cells or of prespores to total cells in cell masses depended on the terminal structures formed; the ratio was unusually high or unusually low in a structure which stopped developing before papilla formation, while the ratio was normal in a structure forimed after that stage. When isolated from a cell mass, prespore cells of the mutant did not dedifferentiate or resumed vegetative growth, indicating that they had lost plasticity of differentiation. The conditioned medium in which the mutant cells had grown was effective in inducing diikzntistion of wild type slug cells into spore-like or 'stalk-like cells.The development of the cellular slime mould Dictyostelium discoideum is initiated by aggregation of individual amebas into a hemispherical cell mass. A papilla develops on the top of the aggregate and then elongates and transforms into a slug shaped cell mass. After a period of migration, the slug constructs a fruiting body consisting of a cellular stalk supporting a mass of spores. During formation of the fruiting body, the anterior prestalk cells of the slug differentiate into stalk cells, while the posterior prespore cells differentiate into spores (14). The prespore cells are identified by the presence of a prespore antigen which is specifically stained with fluorescein conjugated antispore serum (17, 19).Free-living cells which have not aggregated do not differentiate into either spores or stalk cells under normal conditions. When cells disaggregated from a slug are replated without being allowed to reaggregate, they are inhibited from accumulating some stage specific enzymes (13). Similarly, isolated prespore cells lose the prespore antigen and dedifferentiate to become capable of vegetative growth (21). Furthermore, when a slug of a "cell contact temperature-sensitive" mutant was exposed to non-permissive conditions, the cells dedifferentiated in spite of the fact that they were still packed in the cell mass (1 1).BONNER ( Cell contact is required for cell differentiation in this organism.The following observations, however, seem inconsistent with these facts.

show abstract

“…The stage at which differentiation ofspore and stalk cell types begins is poorly defined but it may be as early as late aggregation, before the multicellular structure is complete (9,10). Cells progressing along these developmental pathways are clearly evident in the slug, a polarized structure having a single tip at its anterior end.…”

mentioning

confidence: 99%

Thermosensitive development and tip regulation in a mutant of Dictyostelium discoideum.

Barclay¹,

Henderson²

1982

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A thermosensitive developmental mutant of Dictyostelium discoideum identifies a gene product that is nonessential for cell multiplication but is continuously required during aggregation and the period when multicellular mounds are formed. After mounds form a tip, which has the properties ofan embryonic organizer, this gene product may be nonessential. Surgical removal of the tip from a polarized developing multicellular structure (the slug) leads to emergence of a new tip at the permissive temperature but not at the restrictive temperature. The mutant continues to develop abnormally when mixed with wild-type cells; therefore, a cell-limited rather than an exchangeable factor is altered. Assays show that the mutant has a thermosensitive defect in chemotaxis toward cAMP. The mutation reduces the number of cell surface cAMP receptors expressed at the restrictive temperature without affecting their dissociation constants or their apparent thermostability. The expression of two developmentally regulated enzymes, N-acetylglucosaminidase and cAMP phosphodiesterase, is unaffected by the mutation.

show abstract

Quantitative studies on cell differentiation during morphogenesis of the cellular slime mold Dictyostelium discoideum

Cited by 81 publications

References 14 publications

Fate and regulation of anterior-like cells in Dictyostelium slugs

Fate and regulation of anterior-like cells in Dictyostelium slugs

A Mutant of Dictyostelium Discoideum Capable of Differentiating Without Morphogenesis

Thermosensitive development and tip regulation in a mutant of Dictyostelium discoideum.

Contact Info

Product

Resources

About