Quantitative study of protein coronas on gold nanoparticles with different surface modifications

Cui, Menghua; Liu, Renxiao; Deng, Zhaoyi; Ge, Guanglu; Liu, Ying; Xie, Liming

doi:10.1007/s12274-013-0400-0

Cited by 90 publications

(97 citation statements)

References 26 publications

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“…The results showed that there is negligible protein adsorption on the surface of PEG-Au NPs. Fibrinogen can induce NP aggregation when it interacts with other non-PEG-coated Au NPs, while citrate or thioglycolic acid-coated Au NPs are capable of adsorbing TRF and BSA to form a 6–8 nm thickness corona [81]. The molecular weight of PEG ligands on Au NPs also influenced the bound proteins.…”

Section: Au Np–protein Interactionsmentioning

confidence: 99%

“…TEM and DLS are widely used to measure the thickness of the protein corona on NPs in dried samples and in an aqueous solution, respectively. Au NPs with different modifications can interact with BSA and the hydrodynamic diameter or protein thickness increases determined by DLS and TEM [81]. Moreover, UV–vis–NR spectra can detect significant shifts in the peak position of the SPR of Au NPs before and after protein adsorption.…”

Section: Au Np–protein Interactionsmentioning

confidence: 99%

“…A previous study demonstrated that citrate-stabilized 10 nm Au NPs can induce the activation of the NF- κ B signaling pathway in the murine B-lymphocyte cell line (CH12.LX). This pathway is a way for Au NPs to mediate the inflammatory responses of lymphocytes [81]. We used mesoporous silica and Au NRs to construct a core–shell structure as a promising and multiple theranostic nanocarrier [10, 114].…”

Section: Interaction Of Au Nps With Cellsmentioning

confidence: 99%

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Interaction of gold nanoparticles with proteins and cells

Wang

et al. 2015

Science and Technology of Advanced Materials

169

117

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Gold nanoparticles (Au NPs) possess many advantages such as facile synthesis, controllable size and shape, good biocompatibility, and unique optical properties. Au NPs have been widely used in biomedical fields, such as hyperthermia, biocatalysis, imaging, and drug delivery. The broad application range may result in hazards to the environment and human health. Therefore, it is important to predict safety and evaluate therapeutic efficiency of Au NPs. It is necessary to establish proper approaches for the study of toxicity and biomedical effects. In this review, we first focus on the recent progress in biological effects of Au NPs at the molecular and cellular levels, and then introduce key techniques to study the interaction between Au NPs and proteins. Knowledge of the biomedical effects of Au NPs is significant for the rational design of functional nanomaterials and will help predict their safety and potential applications.

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Section: Au Np–protein Interactionsmentioning

confidence: 99%

Section: Au Np–protein Interactionsmentioning

confidence: 99%

Section: Interaction Of Au Nps With Cellsmentioning

confidence: 99%

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Interaction of gold nanoparticles with proteins and cells

Wang

et al. 2015

Science and Technology of Advanced Materials

169

117

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“…13,35,36,45 Bare nanoparticles are unable to survive within a biological system since they are immediately covered by a layer of proteins from the fluid which forms the hard protein corona and which continues to grow until they attain a stable state, which is often a long-term process as observed in the case of SiQD 1050. 13,35,36,45 Bare nanoparticles are unable to survive within a biological system since they are immediately covered by a layer of proteins from the fluid which forms the hard protein corona and which continues to grow until they attain a stable state, which is often a long-term process as observed in the case of SiQD 1050.…”

Section: Page 17 Of 23 Rsc Advancesmentioning

confidence: 99%

The impact of doped silicon quantum dots on human osteoblasts

et al. 2016

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Silicon (Si) nanostructures allow for the expansion of the application spectrum of this important semiconductor material with respect to the fields of optoelectronics and photonics. At the same time, the significant potential of Si quantum dots (SiQDs) has been revealed in terms of their potential application in the areas of biology and medicine due to their biocompatibility, low toxicity and natural biodegradability unlike currently used semiconductor quantum dots. As far as this study is concerned, SiQDs co-doped with boron and phosphorus were used for the in vitro evaluation of their cytotoxicity in human osteoblasts. Two chemically identical types of SiQD differing in terms of their size and photoluminescence (PL) were studied. They both display long-lasting dispersion in methanol and even in aqueous media as well as PL which is not sensitive either to changes in the environment or surface modifications. Our experiments revealed significant differences between the two types of SiQD tested with concern to their behavior in a cell culture environment depending on increasing concentration (25 -125 µg/ml) and cultivation conditions (the presence or absence of proteins from the fetal bovine serum -component of the cultivation medium). Detailed description of their optical parameters and the evaluation of zeta potential enhance the understanding of the complexities of the in vitro results obtained.

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“…CD spectroscopy uses changes in the chiral properties of a protein to predict changes in its secondary structure. Measured interactions between plasma proteins and NPs can be quantified using several kinetic models and equations (38)(39)(40). All the methods described in Table 2 are quite accessible and straightforward to evaluate nano-bio interface in pure water or a simple buffer system.…”

Section: Figure 3 Active Interactions At the Nano-bio Interface [Inspmentioning

confidence: 99%

How protein coronas determine the fate of engineered nanoparticles in biological environment

Capjak

Goreta

Jurašin

et al. 2017

Archives of Industrial Hygiene and Toxicology

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Nanomedicine is a booming medical field that utilises nanoparticles (NPs) for the development of medicines, medical devices, and diagnostic tools. The behaviour of NPs in vivo may be quite complex due to their interactions with biological molecules. These interactions in biological fluids result in NPs being enveloped by dynamic protein coronas, which serve as an interface between NPs and their environment (blood, cell, tissue). How will the corona interact with this environment will depend on the biological, chemical, and physical properties of NPs, the properties of the proteins that make the corona, as well as the biological environment. This review summarises the main characteristics of protein corona and describes its dynamic nature. It also presents the most common analytical methods to study the corona, including examples of protein corona composition for the most common NPs used in biomedicine. This knowledge is necessary to design NPs that will create a corona with a desired efficiency and safety in clinical use.

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Quantitative study of protein coronas on gold nanoparticles with different surface modifications

Cited by 90 publications

References 26 publications

Interaction of gold nanoparticles with proteins and cells

Interaction of gold nanoparticles with proteins and cells

The impact of doped silicon quantum dots on human osteoblasts

How protein coronas determine the fate of engineered nanoparticles in biological environment

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