Quantitative surface plasmon spectroscopy: Determination of the infrared optical constants of living cells

Zilbershtein, A. Kh.; Golosovsky, M.; Lirtsman, Vladislav; Aroeti, Benjamin; Davidov, D.

doi:10.1016/j.vibspec.2012.01.019

Cited by 17 publications

(30 citation statements)

References 45 publications

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“…20 The procedure involves calibration measurement with known analyte (pure water) that yields the optical constants of the Au film. At the next step we used the same Fresnel equations to find the optical constants (n d , κ d ) of the unknown analyte.…”

Section: Analysis Of the Infrared Spectramentioning

confidence: 99%

“…At the next step we used the same Fresnel equations to find the optical constants (n d , κ d ) of the unknown analyte. 20 …”

Section: Analysis Of the Infrared Spectramentioning

confidence: 99%

“…2(a) and 2(b). 20 The real part of cell refractive index [ Fig. 2(a)] exceeds that of the culture medium (mostly water) due to presence of up to 30% organic substances.…”

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

“…The scattering losses dominate at the shorter wavelengths (λ < 2 μm) and the absorption losses dominate in the mid-infrared range (λ > 2 μm). 20 For nonhomogeneous media such as cells, the effective refractive index sensed by the SP wave is a weighted average in z-direction,…”

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

“…Using our knowledge of the cell optical constants 20 we could make a clever choice of the wavelength range for our living cell studies. Indeed, the infrared wavelength range enables extension of the SP sampling depth up to few microns [ Fig.…”

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

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Surface plasmon-based infrared spectroscopy for cell biosensing

Yashunsky

2012

J. Biomed. Opt

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Abstract. Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utilize the unique properties of the confined infrared waves (i.e., surface plasmon and guided modes) traveling inside the cell layer. The propagation of these waves strongly depends on cell morphology and connectivity. By tracking the resonant wavelength and attenuation of the surface plasmon and guided modes we measure the kinetics of various cellular processes such as (i) cell attachment and spreading on different substrata, (ii) modulation of the outer cell membrane with chlorpromazine, and (iii) formation of intercellular junctions associated with progressive cell polarization. Our method enables monitoring of submicron variations in cell layer morphology in real-time, and in the label-free manner.

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Section: Analysis Of the Infrared Spectramentioning

confidence: 99%

“…At the next step we used the same Fresnel equations to find the optical constants (n d , κ d ) of the unknown analyte. 20 …”

Section: Analysis Of the Infrared Spectramentioning

confidence: 99%

“…2(a) and 2(b). 20 The real part of cell refractive index [ Fig. 2(a)] exceeds that of the culture medium (mostly water) due to presence of up to 30% organic substances.…”

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

Section: Studying Living Cells With Surface Plasmonmentioning

confidence: 99%

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Surface plasmon-based infrared spectroscopy for cell biosensing

Yashunsky

2012

J. Biomed. Opt

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Prism dispersion effects in near‐guided‐wave surface plasmon resonance sensors

Shalabney

Abdulhalim

2012

Annalen der Physik

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Refractive index dispersion causes the light line to curve. As a result it is shown that when the prism is dispersive, an additional dip in the spectral response of Surface Plasmon Resonance (SPR) sensors is observed in the Kretschmann-Raether (KR) configuration. Since the new dip evolves in the infrared (IR) region, it exhibits a high sensitivity to the analyte refractive index (RI) changes and the mode penetrates deeper into the analyte. Adding a thin dielectric layer with high refractive index on top of the metallic layer enables to control the dip location and strength. The two dips shift in opposite directions as the analyte RI changes and therefore when the spectral difference is considered as the measurand, higher RI sensitivity is obtained. The dispersion relation of two thin films bounded by two semi-infinite media is derived when the prism dispersion is considered.

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Micromachined silicon attenuated total reflectance infrared spectroscopy: An emerging detection method in micro/nanofluidics

Karabudak

2013

Electrophoresis

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Microfluidics is an emerging field with various applications. In situ analysis of liquids inside microfluidic channels is an important research subject for any of these applications. On the other hand, attenuated total reflectance infrared spectroscopy (ATR-IR) is a strong surface detection method. Recently, there have been various attempts to use ATR-IR for detection of liquids inside microfluidic channels. Micromachined silicon ATR-IR (μSi-ATR-IR) is an emerging analytical tool for micro/nanofluidics. This paper reviews μSi-ATR-IR micro/nanofluidics detection devices and discusses the application fields, capabilities, advantages, and disadvantages of the method.

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Quantitative surface plasmon spectroscopy: Determination of the infrared optical constants of living cells

Cited by 17 publications

References 45 publications

Surface plasmon-based infrared spectroscopy for cell biosensing

Surface plasmon-based infrared spectroscopy for cell biosensing

Prism dispersion effects in near‐guided‐wave surface plasmon resonance sensors

Micromachined silicon attenuated total reflectance infrared spectroscopy: An emerging detection method in micro/nanofluidics

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