Quantitative trait locus analysis and construction of consensus genetic map for foliar disease resistance based on two recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.)

Abstract

Late leaf spot (LLS) and rust have the greatest impact on yield losses worldwide in groundnut (Arachis hypogaea L.). With the objective of identifying tightly linked markers to these diseases, a total of 3,097 simple sequence repeats (SSRs) were screened on the parents of two recombinant inbred line (RIL) populations, namely TAG 24 × GPBD 4 (RIL-4) and TG 26 × GPBD 4 (RIL-5), and segregation data were obtained for 209 marker loci for each of the mapping populations. Linkage map analysis of the 209 loci resulted in the mapping of 188 and 181 loci in RIL-4 and RIL-5 respectively. Using 143 markers common to the two maps, a consensus map with 225 SSR loci and total map distance of 1,152.9 cM was developed. Comprehensive quantitative trait locus (QTL) analysis detected a total of 28 QTL for LLS and 15 QTL for rust. A major QTL for LLS, namely QTLLLS01 (GM1573/GM1009-pPGPseq8D09), with 10.27–62.34% phenotypic variance explained (PVE) was detected in all the six environments in the RIL-4 population. In the case of rust resistance, in addition to marker IPAHM103 identified earlier, four new markers (GM2009, GM1536, GM2301 and GM2079) showed significant association with the major QTL (82.96% PVE). Localization of 42 QTL for LLS and rust on the consensus map identified two candidate genomic regions conferring resistance to LLS and rust. One region present on linkage group AhXV contained three QTL each for LLS (up to 67.98% PVE) and rust (up to 82.96% PVE). The second candidate genomic region contained the major QTL with up to 62.34% PVE for LLS. Molecular markers associated with the major QTL for resistance to LLS and rust can be deployed in molecular breeding for developing groundnut varieties with enhanced resistance to foliar diseases.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-011-9661-z) contains supplementary material, which is available to authorized users.

Prospecting of efficient rhizobia for peanut inoculation in a Planosol under different vegetation covers

“…Peanut (Arachis hypogaea L.) is a legume of major global importance, being grown in Asia, Africa, North America and South America, where it originated (Sujay et al, 2012;Wang et al, 2012;Mokgehle et al, 2014;Pozzi et *Corresponding author. E-mail: v inicius.agro2008.1@gmail.com.…”

Genetic analysis of foliar disease resistance, yield and nutritional quality traits in groundnut

“…A significant positive association among LLS and rust scores (0.48 to 0.60) was also reported by Anderson et al (1990). Quantitative trait locus (QTL) mapping studies have confirmed this association wherein a major QTL explaining >80% phenotypic variation (PV) for rust resistance on linkage group AhXV, recently assigned to chromosome A03, also explained 68 % PV for LLS in groundnut (Sujay et al, 2012, Pandey et al, 2017 Severe disease during pod development stage results in acute pod yield losses. Infection of LLS and rust starts from 60 DAS, therefore disease scores recorded at 75 DAS are usually low.…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…For example, the first SSRbased genetic linkage map for cultivated groundnut was recently developed for the mapping population, TAG 24 × ICGV 86031 (Varshney et al, 2009b). Subsequently five more genetic maps were developed based on mapping populations segregating for drought and foliar diseases (Gautami et al, 2012a;Ravi et al, 2011;Sujay et al, 2012). A consensus map for drought tolerance related traits with 293 SSR loci (Gautami et al, 2012a) and foliar disease resistance with 225 SSR loci (Sujay et al, 2012), respectively have been developed.…”

“…Subsequently five more genetic maps were developed based on mapping populations segregating for drought and foliar diseases (Gautami et al, 2012a;Ravi et al, 2011;Sujay et al, 2012). A consensus map for drought tolerance related traits with 293 SSR loci (Gautami et al, 2012a) and foliar disease resistance with 225 SSR loci (Sujay et al, 2012), respectively have been developed. Furthermore, international collaborative efforts have resulted in the construction of consensus map with 897 SSR marker loci using genotyping data from 11 mapping populations.…”