Quantitative trait locus mapping of gravitaxis behaviour in<i>Drosophila melanogaster</i>

Desroches, Christie E.; Busto, Macarena; Riedl, Craig A.L.; Mackay, Trudy F. C.; Sokolowski, Marla B.

doi:10.1017/s0016672310000194

Cited by 6 publications

(5 citation statements)

References 42 publications

(55 reference statements)

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“…When startled, adult flies exhibit a negative geotactic behavior, in which flies crawl up, away from the gravitational pull. In Drosophila , mechanosensory bristles and a subset of ch neurons located in the Johnston's organ of the adult antennae regulate sensory perception of Earth's gravitational field (Desroches et al, ). Since Caper dysfunction results in defects in various mechanosensory neurons, we used a well‐established gravitaxis assay, which quantifies climbing speeds after the flies are startled, to determine if Caper dysfunction also results in behavioral defects.…”

Section: Resultsmentioning

confidence: 99%

The RNA‐binding protein caper is required for sensory neuron development in Drosophila melanogaster

Olesnicky

Bono

Bell

et al. 2017

Developmental Dynamics

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Background Alternative splicing mediated by RNA-binding proteins (RBPs) is emerging as a fundamental mechanism for the regulation of gene expression. Alternative splicing has been shown to be a widespread phenomenon that facilitates the diversification of gene products in a tissue specific manner. Although defects in alternative splicing are rooted in many neurological disorders, only a small fraction of splicing factors have been investigated in detail. Results We find that the splicing factor Caper is required for the development of multiple different mechanosensory neuron subtypes at multiple life stages in Drosophila melanogaster. Disruption of Caper function causes defects in dendrite morphogenesis of larval dendrite arborization neurons, neuronal positioning of embryonic proprioceptors, as well as the development and maintenance of adult mechanosensory bristles. Additionally, we find that Caper dysfunction results in aberrant locomotor behavior in adult flies. Transcriptome-wide analyses further support a role for Caper in alternative isoform regulation of genes that function in neurogenesis. Conclusions Our results provide the first evidence for a fundamental and broad requirement for the highly conserved splicing factor Caper in the development and maintenance of the nervous system and provide a framework for future studies on the detailed mechanism of Caper mediated RNA regulation.

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Section: Resultsmentioning

confidence: 99%

The RNA‐binding protein caper is required for sensory neuron development in Drosophila melanogaster

Olesnicky

Bono

Bell

et al. 2017

Developmental Dynamics

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“…Although mammalian models are useful in these studies, the short generation time of Drosophila (around 10 days) could streamline the experimental selection, over several generations, of Drosophila strains that have increased or decreased lifespan and motor activity ( Rose and Graves, 1989 ; Mackay, 2002 ; Desroches et al, 2010 ; Wilson et al, 2013 ), which could enable the identification of polymorphisms associated with age-related muscle dysfunction. Moreover, the analysis of large, genetically homogeneous populations could shed light on the epigenetic and stochastic changes responsible for individual variation in developing age-related muscle dysfunction ( Grover et al, 2009 ).…”

Section: Genetic Propensity To Sarcopeniamentioning

confidence: 99%

Mechanisms of skeletal muscle aging: insights from Drosophila and mammalian models

Demontis

Piccirillo

Goldberg

et al. 2013

Disease Models &Amp; Mechanisms

218

219

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A characteristic feature of aged humans and other mammals is the debilitating, progressive loss of skeletal muscle function and mass that is known as sarcopenia. Age-related muscle dysfunction occurs to an even greater extent during the relatively short lifespan of the fruit fly Drosophila melanogaster. Studies in model organisms indicate that sarcopenia is driven by a combination of muscle tissue extrinsic and intrinsic factors, and that it fundamentally differs from the rapid atrophy of muscles observed following disuse and fasting. Extrinsic changes in innervation, stem cell function and endocrine regulation of muscle homeostasis contribute to muscle aging. In addition, organelle dysfunction and compromised protein homeostasis are among the primary intrinsic causes. Some of these age-related changes can in turn contribute to the induction of compensatory stress responses that have a protective role during muscle aging. In this Review, we outline how studies in Drosophila and mammalian model organisms can each provide distinct advantages to facilitate the understanding of this complex multifactorial condition and how they can be used to identify suitable therapies.

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“…The response to gravity has previously been measured by use of a vertical countercurrent apparatus [17], [46] and a vertical maze [14], [16], [47]–[50]. In addition, flies have been observed as they climbed up a vertical tube [15], [21], [24], [51], [52]; our method (Figure 18) is basically similar, yet “simple” and quantitative.…”

Section: Discussionmentioning

confidence: 84%

Simple Ways to Measure Behavioral Responses of Drosophila to Stimuli and Use of These Methods to Characterize a Novel Mutant

2012

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The behavioral responses of adult Drosophila fruit flies to a variety of sensory stimuli – light, volatile and non-volatile chemicals, temperature, humidity, gravity, and sound - have been measured by others previously. Some of those assays are rather complex; a review of them is presented in the Discussion. Our objective here has been to find out how to measure the behavior of adult Drosophila fruit flies by methods that are inexpensive and easy to carry out. These new assays have now been used here to characterize a novel mutant that fails to be attracted or repelled by a variety of sensory stimuli even though it is motile.

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Quantitative trait locus mapping of gravitaxis behaviour inDrosophila melanogaster

Cited by 6 publications

References 42 publications

The RNA‐binding protein caper is required for sensory neuron development in Drosophila melanogaster

The RNA‐binding protein caper is required for sensory neuron development in Drosophila melanogaster

Mechanisms of skeletal muscle aging: insights from Drosophila and mammalian models

Simple Ways to Measure Behavioral Responses of Drosophila to Stimuli and Use of These Methods to Characterize a Novel Mutant

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