Quantitative validation of nicotine production in tea (Camellia sinensis L.)

Ikka, Takashi; Yamashita, Hiroto; Kurita, Ikuya; Tanaka, Yasuno; Taniguchi, Fumiya; Ogino, Akifumi; Takeda, Kazuya; Horie, Nobuhiro; Hara, Hiroshi; Nanjo, Fumio; Morita, Akio

doi:10.1371/journal.pone.0195422

Cited by 8 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These rather low concentrations, without any hint of biological activity are also known for other (food plants) like tea ( Camellia sinensis ), carrot, pear, strawberry or plants from the Solanaceae family (besides the well‐known Nicotiana spp. which shows extremely high values in the g/kg range) …”

Section: Resultsmentioning

confidence: 96%

HILIC HPLC‐ESI‐MS/MS identification and quantification of the alkaloids from the genus Equisetum

Tipke

Bücker

Middelstaedt

et al. 2019

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction The plant family Equisetaceae (Equisetopsida, Monilophyta; common name: horsetails) is part of an ancient group of spore producing plants. Today, Equisetum is the only surviving genus comprising 15 species in two subgenera (Equisetum and Hippochaete). Several unique alkaloids are described to occur in this genus, so far there is very little data on the occurrence and the amount of those alkaloids for the different species. Objective To establish an extraction method and an analytical method to detect and quantify the relevant Equisetum‐type alkaloids and to create a quantitative data set on the alkaloid content of all Equisetum species worldwide. Methodology Hydrophilic interaction liquid chromatography high‐performance liquid chromatography tandem mass spectrometry (HILIC HPLC‐MS/MS) in electrospray ionisation (ESI) positive mode was used to analyse and quantify the alkaloid content of 68 Equisetum samples. Results The presence of nicotine in at least one sample of each Equisetum species could be demonstrated. The total nicotine amount rarely exceeded 250 μg/kg and 50 μg/kg for the subgenus Equisetum and Hippochaete, respectively. Besides nicotine, Equisetum‐type alkaloids (mainly palustrine and palustridiene) were only detected in three species, namely E. palustre, E. bogotense and E. giganteum. For E. giganteum, palustridiene was detected at levels around the limit od detection (LoD) (25 μg/kg), whereas in E. palustre and E. bogotense, both alkaloids (palustrine and palustridiene) were detected at much higher levels (20–800 mg/kg). Conclusions All Equisetum species occurring worldwide were successfully subjected to a detailed qualitative and quantitative alkaloid analysis using a newly developed HILIC‐HPLC‐ESIpos‐MS/MS approach. The data set can be used to distinguish different Equisetum‐chemotypes.

show abstract

Section: Resultsmentioning

confidence: 96%

HILIC HPLC‐ESI‐MS/MS identification and quantification of the alkaloids from the genus Equisetum

Tipke

Bücker

Middelstaedt

et al. 2019

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…Other possible explanations include diet and/or pollution. Low levels of nicotine are present in a number of frequently consumed vegetables, more specifically of the Solanaceae and Brassicaceae family (eggplant, bell peppers, chili peppers, potatoes, tomatoes, tomatillos, and cauliflower), as well tea leaves 27,28 . On average, each fresh fruit of this vegetables contains nicotine at 2–7 μg/kg, and dietary sources may provide a maximum intake of 2.25 μg/day.…”

Section: Discussionmentioning

confidence: 99%

“…Low levels of nicotine are present in a number of frequently consumed vegetables, more specifically of the Solanaceae and Brassicaceae family (eggplant, bell peppers, chili peppers, potatoes, tomatoes, tomatillos, and cauliflower), as well tea leaves. 27 , 28 On average, each fresh fruit of this vegetables contains nicotine at 2–7 μg/kg, and dietary sources may provide a maximum intake of 2.25 μg/day. Consumption of these plants for many years could explain the low cotinine level detected in our nonsmoker participants, who reported daily consumption of these foods.…”

Section: Discussionmentioning

confidence: 99%

Cotinine levels influence the risk of rupture of brain aneurysms

Missori

Sousa

Ambrosone

et al. 2022

Acta Neuro Scandinavica

View full text Add to dashboard Cite

The relationship between nicotine levels in smokers and brain aneurysm has never been determined. To assess the levels of cotinine in smoker patients with ruptured and unruptured brain aneurysm and the risk of aneurysm rupture we quanti ed cotinine levels in smoker patients with ruptured or unruptured brain aneurysms. We identi ed a control group of smokers and nonsmokers without brain aneurysm. Out of 182 participants cerebral aneurysms were statistically signi cantly associated with smoking (P<0.001) and female sex (P=0.006). Cotinine levels were signi cantly correlated with both the presence (P=0.009) and the rupture (P=0.002) of brain aneurysms. Compared with nonsmokers, smokers had a 5-fold higher risk of having a brain aneurysm (OR, 5.72; 95% CI, 2.96-11.07; P<0.001). The risk of rupture of brain aneurysms increased by 50% with each cotinine unit and was 4-fold higher with cotinine levels between 4 and 6 (OR, 3.75; 95% CI, 1.48-9.53; P=0.005). With increasing age, the cotinine level decreased (P <0.001, rho= -0.28), declining by 2% with each year of age. In the whole population, the probability of a ruptured aneurysm in patients with cotinine levels between 4 and 6 was higher than in those with levels between 0 and 3 (OR, 5.55; 95% CI, 1.08-28.5; P=0.040). Our results suggest that high cotinine levels in smokers with brain aneurysm, rather than size, are signi cantly associated with high rupture risk. Cotinine levels decrease with age, possibly reducing the risk of formation and rupture of a brain aneurysm.

show abstract

“…As an aqueous product extracted from C. sinensis, tea has become one of the most popular beverages due to its pleasant flavor, taste, and bioactive ingredients for health (Ikka et al, 2018;Li et al, 2015). Previous studies have reported that tea leaves contain important bioactive compounds such as polyphenols, alkaloids, caffeine, and theanine.…”

Section: Introductionmentioning

confidence: 99%

Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis)

et al. 2021

View full text Add to dashboard Cite

Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of 5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.

show abstract

Quantitative validation of nicotine production in tea (Camellia sinensis L.)

Cited by 8 publications

References 31 publications

HILIC HPLC‐ESI‐MS/MS identification and quantification of the alkaloids from the genus Equisetum

HILIC HPLC‐ESI‐MS/MS identification and quantification of the alkaloids from the genus Equisetum

Cotinine levels influence the risk of rupture of brain aneurysms

Catechin Contents, Antioxidant and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis)

Contact Info

Product

Resources

About