Quantity of the Tetracycline Resistance Gene <i>tet</i>(M) Differs Substantially between Meat at Slaughterhouses and at Retail

Hölzel, Christina; Huther, Sabine Katharina; Schwaiger, Karin; Kämpf, Peter; Bauer, Johann

doi:10.1111/j.1750-3841.2011.02239.x

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…This is an important matter considering the high prevalence of these genes in gram‐negative bacteria and their abilities to be transferred to human bacteria (Aarestrup 2005). This idea of monitoring a gene pool instead of single bacteria was already highlighted by Hölzel and others (2011); however, they focused their research on the quantification of the tetM gene, which is more frequent in gram‐positive bacteria.…”

Section: Resultsmentioning

confidence: 99%

Direct Quantification and Distribution of Tetracycline‐Resistant Genes in Meat Samples by Real‐Time Polymerase Chain Reaction

Guarddon¹,

Miranda²,

Vázquez³

et al. 2012

Journal of Food Science

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: The evolution of antimicrobial‐resistant bacteria has become a threat to food safety and methods to control them are necessary. Counts of tetracycline‐resistant (TR) bacteria by microbiological methods were compared with those obtained by quantitative PCR (qPCR) in 80 meat samples. TR Enterobacteriaceae counts were similar between the count plate method and qPCR (P= 0.24), whereas TR aerobic mesophilic bacteria counts were significantly higher by the microbiological method (P < 0.001). The distribution of tetA and tetB genes was investigated in different types of meat. tetA was detected in chicken meat (40%), turkey meat (100%), pork (20%), and beef (40%) samples, whereas tetB was detected in chicken meat (45%), turkey meat (70%), pork (30%), and beef (35%) samples. The presence of tetracycline residues was also investigated by a receptor assay. This study offers an alternative and rapid method for monitoring the presence of TR bacteria in meat and furthers the understanding of the distribution of tetA and tetB genes.

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Section: Resultsmentioning

confidence: 99%

Direct Quantification and Distribution of Tetracycline‐Resistant Genes in Meat Samples by Real‐Time Polymerase Chain Reaction

Guarddon¹,

Miranda²,

Vázquez³

et al. 2012

Journal of Food Science

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“…Tn916-Tn1545 family) and can therefore easily be transferred from one bacterial species to another (Doherty et al, 2000). Accordingly, tet(M) has been detected in numerous food matrices, including dairy (Flórez et al, 2014) and meat products (Hölzel et al, 2011). Interestingly, in the present study, two samples (MW 1.1 and MW 1.2) did not carry any other AR gene than tet(M).…”

Section: Quantitative Detection Of Antibiotic Resistance Genes In Insect Samplesmentioning

confidence: 43%

“…Compared to other food matrices, the resistance gene quantity carried by edible insects varies within the same range. For example, the tet(M) gene was reported to be present almost up to 7 and 8 log copies/cm 2 chicken and pork meat, respectively (Hölzel et al, 2011). Likewise, for cheeses, the tet(S) gene was observed ranging from 4.5 up to 8 log copies/g (Manuzon et al, 2007).…”

Section: Quantitative Detection Of Antibiotic Resistance Genes In Insect Samplesmentioning

confidence: 99%

Real-time PCR detection and quantification of selected transferable antibiotic resistance genes in fresh edible insects from Belgium and the Netherlands

Vandeweyer¹,

Milanović

Garofalo

et al. 2019

International Journal of Food Microbiology

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The occurrence of antibiotic resistance genes in foodstuffs involves a human health risk. Fresh edible insects present an emerging source of human food but they were not yet assessed in a quantitative way for antibiotic resistances as a matter of food safety. In this study, a real-time quantitative PCR assessment was optimised to detect and quantify relevant transferable antibiotic resistance genes [tet(O, K, M, S) and erm(B)] in edible insects. Subsequently, the technology was applied on 30 fresh insect samples, including two mealworm species and two cricket species from different production batches and rearing companies in Belgium and the Netherlands. The sampling periods and the post-harvest treatments applied were also taken into account. Results showed that mealworms contained, on average, higher numbers of tet(K), tet(M), and tet(S) genes than crickets, but tet(O) was almost uniquely present in crickets. The erm(B) gene was only detected in one mealworm sample and the tet(K) gene showed higher abundances in samples originating from the Netherlands than in samples from Belgium. A large difference in antibiotic resistance profile was revealed between mealworms and crickets, but not between different mealworm species or cricket species. Species-specific microbiomes and insect feed may have contributed to this distinction. Interestingly, important correlations between the presence of some tet genes and the microbiota previously encountered in the investigated edible insects were uncovered. While a geographical distribution was observed for the tet(K) gene, post-harvest treatments and sampling period were not shown to have a significant influence on the occurrence of the antibiotic resistance genes considered. In conclusion, insects may carry considerable amounts of antibiotic resistance genes, but the health risk in terms of antibiotic resistances is comparable to other food matrices.

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Prevalence of antibiotic-resistant enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany

Schwaiger

Huther

Hölzel

et al. 2012

International Journal of Food Microbiology

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Quantity of the Tetracycline Resistance Gene tet(M) Differs Substantially between Meat at Slaughterhouses and at Retail

Cited by 4 publications

References 53 publications

Direct Quantification and Distribution of Tetracycline‐Resistant Genes in Meat Samples by Real‐Time Polymerase Chain Reaction

Direct Quantification and Distribution of Tetracycline‐Resistant Genes in Meat Samples by Real‐Time Polymerase Chain Reaction

Real-time PCR detection and quantification of selected transferable antibiotic resistance genes in fresh edible insects from Belgium and the Netherlands

Prevalence of antibiotic-resistant enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany

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