Quantum Dot Biolabeling Coupled with Immunomagnetic Separation for Detection of <i>Escherichia c</i><i>oli</i> O157:H7

Su, Xiaoli; Li, Yanbin

doi:10.1021/ac049442+

Cited by 233 publications

(132 citation statements)

References 29 publications

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“…However, multi-photon microscopy does have the potential, especially in the near IR wavelength range, to detect the onset of biofilm formation provided one could label the biofilm cells in situ. Hypothetically, one could imagine periodically injecting an implant patient with immunoconjugated quantum dots (Su and Li, 2004;Yang and Li, 2006) that are specific to potential implant-colonizing bacteria. The circulating immuno-quantum dots would locate targeted biofilm bacteria thus allowing early stage multi-photon detection of an implant infection.…”

Section: Detecting Medical Biofilmsmentioning

confidence: 99%

Medical biofilms

Bryers

2008

Biotech & Bioengineering

666

527

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For more than two decades, Biotechnology and Bioengineering has documented research focused on natural and engineered microbial biofilms within aquatic and subterranean ecosystems, wastewater and waste-gas treatment systems, marine vessels and structures, and industrial bioprocesses. Compared to suspended culture systems, intentionally engineered biofilms are heterogeneous reaction systems that can increase reactor productivity, system stability, and provide inherent cell:product separation. Unwanted biofilms can create enormous increases in fluid frictional resistances, unacceptable reductions in heat transfer efficiency, product contamination, enhanced material deterioration, and accelerated corrosion. Missing from B&B has been an equivalent research dialogue regarding the basic molecular microbiology, immunology, and biotechnological aspects of medical biofilms. Presented here are the current problems related to medical biofilms; current concepts of biofilm formation, persistence, and interactions with the host immune system; and emerging technologies for controlling medical biofilms.

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Section: Detecting Medical Biofilmsmentioning

confidence: 99%

Medical biofilms

Bryers

2008

Biotech & Bioengineering

666

527

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“…In 2003, Kloepfer et al were the first to use QDs for bacterial labeling. 126 Since this pioneering work, the use of QDs has been more widespread and is now used for labeling, detection, and quantification of Mycobacterium bovis, Bacillus Calmette-Guérin, 149 Escherichia coli O157:H7, 150 and …”

Section: Imaging Contrast Enhancers and Oral Cancersmentioning

confidence: 99%

Nanotechnology in dentistry: prevention, diagnosis, and therapy

Neel¹,

Bozec

Pérez³

et al. 2015

IJN

102

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Nanotechnology has rapidly expanded into all areas of science; it offers significant alternative ways to solve scientific and medical questions and problems. In dentistry, nanotechnology has been exploited in the development of restorative materials with some significant success. This review discusses nanointerfaces that could compromise the longevity of dental restorations, and how nanotechnolgy has been employed to modify them for providing long-term successful restorations. It also focuses on some challenging areas in dentistry, eg, oral biofilm and cancers, and how nanotechnology overcomes these challenges. The recent advances in nanodentistry and innovations in oral health-related diagnostic, preventive, and therapeutic methods required to maintain and obtain perfect oral health, have been discussed. The recent advances in nanotechnology could hold promise in bringing a paradigm shift in dental field. Although there are numerous complex therapies being developed to treat many diseases, their clinical use requires careful consideration of the expense of synthesis and implementation.

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“…Many groups have reported the application of nanotechnologies for pathogen detection. Fluorescent nanoparticles or quantum dots that have several advantages over conventional organic dyes including high quantum yield and brightness, photostability, and resistance to chemical degradation were used to detect E. coli O157:H7 (Su and Li, 2004;Wang et al, 2012;Zhu et al, 2012), Salmonella Kuang et al, 2013), and Listeria monocytogenes (Wang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7

Kim¹,

Moon²,

Park³

et al. 2013

Journal of Biosystems Engineering

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Purpose: This study was performed to develop a lateral flow strip sensor for the detection of pathogenic Escherichia coli O157:H7 in various samples. Also, feasibility of using an image analysis method to improve the interpretation of the strip sensor was evaluated. Methods: The lateral flow strip sensor has been fabricated based on nitrocellulose lateral-flow membrane. Colloidal gold and E. coli O157:H7 antibodies were used as a tag and a receptor, respectively. Manually spotted E. coli O157:H7 antibody and anti-mouse antibody on nitrocellulose membrane were used as test and control dots, respectively. Feasibility of the lateral flow strip sensor to detect E. coli O157:H7 were evaluated with serially diluted E. coli O157:H7 cells in PBS or food samples. Test results of the lateral flow strip sensor were measured with an image analysis method. Results: The intensity of the test dot started to increase with higher concentration of the cells were introduced. The sensitivities of the sensor were both 10 4 CFU/mL Escherichia coli O157:H7 spiked in PBS and in chicken meat extract, respectively. Conclusions: The lateral flow strip sensor and image analysis method could detect E. coli O157:H7 in 20 min, which is significantly quicker than conventional plate counting method.

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Quantum Dot Biolabeling Coupled with Immunomagnetic Separation for Detection of Escherichia coli O157:H7

Cited by 233 publications

References 29 publications

Medical biofilms

Medical biofilms

Nanotechnology in dentistry: prevention, diagnosis, and therapy

Image Analysis of a Lateral Flow Strip Sensor for the Detection of Escherichia coli O157:H7

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